Healing of articular cartilage is a major clinical challenge as it also lacks a direct vasculature and nerves, and carries a limited number of resident chondrocytes that do not proliferate easily. Damaged articular cartilages are usually replaced by fibrocartilages, which are mechanically and structurally weaker and less resilient. Regenerative medicine involving stem cells is considered to have a definitive potential to overcome the limitations associated with the currently available surgical methods of cartilage repair. Among various stem cell types, mesenchymal stem cells (MSCs) are preferred for clinical applications. These cells can be readily derived from various sources and have the ability to trans-differentiate into various tissue-specific cells, including those of the cartilage by the process of chondrogenesis. Compared to embryonic or induced pluripotent stem cells (iPSCs), no ethical or teratogenic issues are associated with MSCs. These stem cells are being extensively evaluated for the treatment of joint affections and the results appear promising. Unlike human medicine, in veterinary medicine, the literature on stem cell research for cartilage regeneration is limited. This review, therefore, aims to comprehensively discuss the available literature and pinpoint the achievements and limitations associated with the use of MSCs for articular cartilage repair in animal species.

Effects of Midazolam or Midazolam-Fentanyl on Sedation and Analgesia Produced by Intramuscular Dexmedetomidine in Dogs

Articles | Veterinární medicína | Agricultural Journals

Summary: Objectives: The objectives of the study were to determine the mechanical properties of a customized buffalo interlocking nail (BIN), intact buffalo tibia, and ostectomized tibia stabilized with BIN in different configurations, as well as to assess the convenience of interlocking nailing in buffalo tibia.Methods: The BIN (316L stainless steel, 12 mm diameter, 250 mm long, nine-hole solid nails with 10° proximal bend) alone was loaded in compression and three-point bending (n = 4 each); intact tibiae and ostectomized tibiae (of buffaloes aged 5–8 years, weighing 300–350 kg) stabilized with BIN using 4.9 mm standard or modified locking bolts (4 or 8) in different configurations were subjected to axial compression, cranio-caudal three-point bending and torsion (n = 4 each) using a universal testing machine. Mechanical parameters were determined from load-displacement curves and compared using Kruskal-Wallis test (p <0.05).Results: Intact tibiae were significantly stronger than BIN and bone-BIN constructs in all testing modes. The strength of fixation constructs with eight locking bolts was significantly more than with four bolts. Overall strength of fixation with modified locking bolts was better than standard bolts. Based on technical ease and biomechanical properties, cranio-caudal insertion of bolts into the bone was found better than medio-lateral insertion.Clinical significance: The eight bolt BINbone constructs could be useful to treat tibial fractures in large ruminants, especially buffaloes.