Arthropods play a crucial role in ecological processes and agricultural productivity. Soil physicochemical properties, indicators of soil health, are closely linked to arthropod communities. Gravel-sand mulching, commonly employed in arid farming, initially enhances water retention and temperature regulation but may contribute to land degradation with prolonged use. This study investigated how varying tillage durations affected soil properties and arthropod diversity under gravel-sand mulching. The analysis employed multiple comparison methods, covariance analysis (ANCOVA), non-metric multidimensional scaling (NMDS), and redundancy analysis (RDA). The results indicated that while soil fertility was better preserved in cultivated fields compared to in the desert grassland, arthropod diversity significantly decreased with longer cultivation periods. A total of 1099 arthropods from 79 species were sampled, by Barber trap. The highest diversity was observed in native grassland (NG), with 305 arthropods from 39 species, while tillage 21 years (GPS-21Y) exhibited the lowest diversity, with only 103 arthropods from 6 species. Dominant species included the carnivores Labidura japonica and Cataglyphis aenes. The analysis revealed low similarity in arthropod communities between GPS-21Y and other fields and high similarity in soil physicochemical properties between NG and the transition zone (STZ). RDA showed available potassium (APP) was negatively correlated with arthropod species diversity and concentration, total Nitrogen (TN) was positively correlated with arthropod species diversity but negatively correlated with species concentration, total phosphorus (TP) was negatively correlated with arthropod species diversity and concentration. This study provides insights into the relationship between maintaining soil fertility and supporting arthropod diversity in grassland agriculture. While soil fertility and arthropod diversity were correlated, continuous cropping practices negatively impacted arthropod diversity, offering valuable information for pest management and sustainable agricultural practices.

Megalurothrips usitatus (Bagnall) is one of the most important pests harming alfalfa (Medicago sativa L.) in Ningxia. In the field, Orius strigicollis (Poppius) is abundant. It has a high search efficiency and consumption rate of prey on a wide range of thrips and is often used as a biological control agent for these insects. To understand the predatory function and biological control potential of O. strigicollis on M. usitatus, the predatory ability of adults of O. strigicollis on second-instar larvae and adults of M. usitatus was measured under indoor conditions, and the Holling II and Hassell–Varley models were fitted to calculate the predatory function response, search efficiency, intraspecific interference, and predation preference of O. strigicollis on second-instar larvae and adults of M. usitatus. Our results showed that the predatory ability of O. strigicollis on second-instar larvae of M. usitatus was 78.62, and the theoretical maximum daily predation was 76.92. The predatory ability of O. strigicollis on adults of M. usitatus was 52.79, and the theoretical maximum daily predation was 52.62. The daily predation of O. strigicollis was positively correlated with prey density and negatively correlated with search efficiency. The rate of predatory action on prey decreased with an increasing O. strigicollis density, while the intensity of apportioned competition increased with an increasing O. strigicollis density. The results of the prey preference experiment showed that the preference of O. strigicollis for second-instar larvae of M. usitatus was significantly higher than for adults of M. usitatus. In conclusion, the indoor experiment data can provide an effective reference for the use of O. strigicollis to control M. usitatus infestation, providing an important biological control agent for controlling M. sativa thrips.

The desert steppe in Ningxia is the largest natural steppe type in the region, characterized by a fragile ecological environment and low carrying capacity. Gravel–sand mulching, a local agricultural practice, involves using a sand and gravel covering layer to maintain soil warmth and moisture. However, this method has led to ecological problems, such as habitat loss and biodiversity loss. Tenebrionidae, one of the most widely distributed beetle families, is commonly used to indicate habitat degradation and is a significant steppe pest in desert steppe areas. This study, conducted in the gravel–sand mulching areas of Shapotou District, Zhongwei City, Ningxia, classified the habitat loss from low to high in these areas into five grades (I-V) and examined the effects of habitat loss on Tenebrionidae. We collected 6565 Tenebrionidae, with Blaps femoralis, Anatolica nureti, and Pterocoma vittata being the dominant species. The findings reveal that habitat loss grade I had the highest abundance, diversity index, and evenness index of Tenebrionidae, significantly higher than those of grades II–IV. Habitat loss had a significant negative effect on Tenebrionidae abundance, a significant positive effect on the richness index, no significant effect on the vegetation diversity index, a significant positive effect on soil available potassium (APP), and a significant negative effect on soil total phosphorus (TP). Redundancy analysis indicated a positive correlation between Tenebrionidae abundance and the vegetation diversity index; a negative correlation between Tenebrionidae richness, the diversity index, and vegetation indices; a positive correlation between Tenebrionidae abundance and soil TP; and a negative correlation between the Tenebrionidae diversity index and soil TP and soil APP. These findings will contribute to biodiversity conservation and ecological restoration and provide a theoretical basis for steppe management, sustainable agricultural development, and pest monitoring in desert steppe environments.

To investigate the effects of seasonal grazing on ground-dwelling insect communities in desert steppe, this study conducted a controlled experiment in the desert steppe of Yanchi County, Ningxia, during 2022–2023. Five grazing regimes were established: spring-summer grazing (Sp+Su), spring-autumn grazing (Sp+Au), summer-autumn grazing (Su+Au), year-round continuous grazing (Annual), and no grazing (Control, CK). Insects were collected using pitfall traps and categorized into herbivorous and predatory functional groups. Combined with monitoring of vegetation community structure, we analyzed the regulatory mechanisms of grazing on insect diversity. The results showed that different grazing regimes had significantly divergent effects on herbivorous and predatory insects. Herbivorous insect diversity was significantly highest under the Annual grazing regime, while Sp+Au grazing effectively controlled herbivorous insect occurrence, resulting in the lowest abundance. Predatory insects exhibited the highest abundance but the lowest diversity under Su+Au grazing, whereas the CK regime increased their species richness. Beta diversity analysis indicated that total replacement diversity (Repl) was dominant, suggesting that grazing primarily influenced community structure by altering species composition rather than changing species number. Non-metric multidimensional scaling (NMDS) results revealed clustering characteristics of insect community structures under different grazing regimes. Redundancy analysis (RDA) and generalized additive models (GAMs) identified vegetation height and predatory insect abundance as key factors driving changes in herbivorous insects. Vegetation density and biomass exhibited nonlinear regulatory effects on herbivorous insects. Based on these findings, we recommend adopting either a hybrid strategy of “year-round continuous grazing combined with seasonal rest” or specifically the “spring + autumn” (Sp+Au) grazing regime. These approaches aim to synergistically achieve the goals of pest control and biodiversity conservation in desert steppe ecosystems.

This study explored the beta diversity patterns of tenebrionid beetles and their driving factors across four grassland types in Ningxia. A total of 32 monitoring sites were surveyed, capturing 5328 individuals from 47 tenebrionid species. Permutation multivariate dispersion (PERMDISP) and non-metric multidimensional scaling (NMDS) revealed significant differences in community composition among the four grassland types (Stress = 0.091). Beta diversity decomposition indicated that species replacement primarily drives beta diversity among four temperate grassland types. Canonical Correspondence Analysis (CCA) showed that mean temperature, field water holding capacity, total soil phosphorus, biomass of Asteraceae, biomass of Leguminosae, and frequency of Leguminosae significantly explained variations in dominant species abundance (p < 0.05). Mantel tests revealed that beta diversity was positively correlated with differences in mean temperature, field water holding capacity, total soil phosphorus, and Asteraceae biomass (p < 0.01), but negatively correlated with Leguminosae frequency (p < 0.05). Overall, daily mean temperature was the primary driver of diversity, with synergistic effects from climate, elevation, soil, and vegetation. This study provides a comprehensive analysis of environmental factors influencing tenebrionid diversity, offering insights for grassland biodiversity conservation.

The laboratory toxicity and physiological effects of three biopesticides—the entomopathogenic fungus Beauveria bassiana, the biocontrol bacterium Bacillus safensis, and the botanical insecticide Artemisia ordosica essential oil—were evaluated against Megalurothrips usitatus, a major pest of alfalfa. All three biopesticides caused concentration-dependent mortality in M. usitatus. Among them, B. bassiana showed the highest corrected mortality (88.42%) and the shortest median lethal time (LT₅₀ = 4.51 days), indicating strong insecticidal activity. The median lethal concentration (LC₅₀) values at 7 days post-treatment were 4.48 × 10⁵ CFU/mL for B. bassiana, 1.67 × 10⁵ CFU/mL for B. safensis, and 2.907 mg/L for A. ordosica essential oil. Moreover, exposure to high concentrations of each agent significantly altered the activities of the protective enzyme peroxidase (POD) and the detoxifying enzyme glutathione S-transferase (GST). Our study supports the use of these biopesticides for thrips management and provides a scientific basis for the eco-friendly and sustainable control of alfalfa pests.