The endemic Labeobarbus species in Lake Tana are severely affected by anthropogenic pressures. The implementation of fisheries management is, therefore, vital for their sustainable exploitation. This study aimed at investigating the catch distribution and size at 50% maturity (FL_50%) of the Labeobarbus species. Samples were collected monthly from May 2016 to April 2017 at four sites. The relative abundance, catch per unit effort (CPUE), and size distribution of these species was computed, and logistic regression was used to calculate FL_50%. Of the 15 species observed, five species constituted 88% of the total catch. The monthly catch of the Labeobarbus spp. declined by more than 85% since 1993 and by 76% since 2001. Moreover, the CPUE of Labeobarbus has markedly decreased from 63 kg/trip in 1991–1993 to 2 kg/trip in 2016–2017. Additionally, large size specimens (≥30 cm fork length) were rarely recorded, and FL_50% of the dominant species decreased. This suggests that the unique species flock may be threatened by extinction. Given the size distribution of the species, the current social context, and the need for a continuous supply of fish for low-income communities, a mesh-size limitation represents a more sustainable and acceptable management measure than a closed season. This paper illustrates the tension between sustainable development goal (SDGs) 1—No Poverty, 2—Zero Hunger, and 8—Decent Work and Economic Growth in Bahir Dar City on the one hand, and SDG’s 11—Sustainable Cities and Communities, 12—Responsible Consumption and Production, and 14—Life Below Water on the other hand. A key for the local, sustainable development of the fisheries is to find a balance between the fishing activities and the carrying capacity of the Lake Tana. Overfishing and illegal fishing are some of the major threats in this respect.

The analysis of fish age data is vital for the successful conservation of fish. Attempts to develop optimal management strategies for effective conservation of the endemic Labeobarbus species are strongly affected by the lack of accurate age estimates. Although methodological studies are key to acquiring a good insight into the age of fishes, up to now, there have not been any studies comparing different methods for these species. Thus, this study aimed at determining the best method for the endemic Labeobarbus species. Samples were collected from May 2016 to April 2017. Asteriscus otoliths from 150 specimens each of L. intermedius, L. tsanensis, L. platydorsus, and L. megastoma were examined. Six methods were evaluated; however, only three methods resulted in readable images. The procedure in which whole otoliths were first submerged in water, and subsequently placed in glycerol to take the image (MO1), was generally best. Except for L. megastoma, this method produced the clearest image as both the coefficient of variation and average percentage error between readers were lowest. Furthermore, except for L. megastoma, MO1 had high otolith readability and no systematic bias. Therefore, we suggest that MO1 should be used as the standard otolith preparation technique for the first three species, while for L. megastoma, other preparation techniques should be evaluated. This study provides a reference for researchers from Africa, particularly Ethiopia, to develop a suitable otolith preparation method for the different tropical fish species.