Silphium integrifolium Michx. (Asteraceae family), commonly known as rosinweed, is a tall (up to 200 cm), long-lived perennial prairie species native to eastern North America. It is studied in various research centers for its potential as an oilseed, forage, honey and technical crop. The goal of this study was to evaluate the silage quality of S. integrifolium grown under the climatic conditions of Moldova and its value as forage for ruminants and substrate for biomethane production. An introduced ecotype of S. integrifolium was cultivated in the experimental plots of the “Alexandru Ciubotaru” National Botanical Garden (Institute). Green biomass was harvested at the early flowering stage from three-year-old plants. Whole plants were chopped and ensiled in sealed containers. After 45 days, silage samples were analyzed for sensory, fermentation and nutrient characteristics according to standard laboratory procedures and the Moldovan Standard SM 108. Biochemical composition was determined using near-infrared spectroscopy (PERTEN DA 7200) at the Institute for Grassland, Braşov. Analyzed parameters included: crude protein, crude fiber, crude ash, total soluble sugars, acid detergent fiber, neutral detergent fiber and acid detergent lignin. Hemicellulose, cellulose, digestible dry matter, digestible energy, metabolizable energy, net energy for lactation and relative feedusing the methods described by Badger et al. (1979), and the biochemical methane potential – according to Dandikas et al. (2015). Results showed that the silage had a pH of 4.54, and contained 8.1 g/kg acetic acid, 32.4 g/kg lactic acid and 0.03 g/kg butyric acid. The silage dry matter nutrients 12.6 % CP, 10.6 % ash, 35.5 % CF, 37.9 % ADF, 58.3 % NDF, 4.6 % ADL, 33.0 % Cel, 20.4 % HC and 2.3 % TSS, with 594 g/kg DDM, 11.76 MJ/kg DE, 9.66 MJ/kg ME, and 5.79 MJ/kg NEl. The C/N ratio of substrate was 24.6, and the biochemical methane potential reached 297 L/kg DM or 332 L/kg ODM. Silphium integrifolium silage exhibits favorable biochemical properties, making it a promising alternative fermented feed for ruminants and viable substrate for biomethane production.