Against the backdrop of advancing ecological civilization and increasing public interest in reconnecting with nature, this study examines fireflies—iconic insects cherished for their natural charm—as valuable landscape resources. This study was conducted in Dalingshan Forest Park, Dongguan (Pearl River Delta), using the Forest Science Trail as a sampling site. Surveys combining line transect and point count methods were employed to analyze firefly species composition, adult activity patterns, and flight characteristics. Key findings include: (1) Four species were identified—Asymmetricata circumdata, Pygoluciola qingyu, Aquatica analis, and Luciola satoi—three of which were observed along the trail; (2) Adults appeared sporadically after 19:00, with peak activity occurring between 19:30 and 20:00, showing minor interspecific variation; (3) Although flight height varied slightly among species, most activities concentrated within 0–1.5 m, corresponding to herbaceous and shrub layers; (4) Distinct flight patterns were observed: A. circumdata displayed prolonged intermittent flights, while P. qingyu and L. satoi exhibited shorter perching-based flights. These results provide a scientific basis for firefly habitat conservation, biodiversity promotion, and the sustainable integration of firefly landscapes into nature education and ecotourism.