Cd is the third major contaminant of greatest hazard to the environment after mercury and lead and is considered as the only metal that poses health risks to both humans and animals at plant tissue concentrations that are generally not phytotoxic.

Different nitrogen (N) sources have been reported to significantly affect the activities and expressions of N metabolism enzymes and mineral elements concentrations in crop plants. However, molybdenum-induced effects in winter wheat cultivars have still not been investigated under different N sources. Here, a hydroponic study was carried out to investigate these effects on two winter wheat cultivars (‘97003’ and ‘97014’) as Mo-efficient and Mo-inefficient, respectively, under different N sources (NO₃⁻, NH₄NO₃, and NH₄⁺). The results revealed that the activities of nitrate reductase (NR) and nitrite reductase (NiR) followed the order of NH₄NO₃ > NO₃⁻ > NH₄⁺ sources, while glutamine synthetase (GS) and glutamate synthase (GOGAT) followed the order of NH₄⁺ > NH₄NO₃ > NO₃⁻ in both the wheat cultivars. However, Mo-induced effects in the activities and expressions of N metabolism enzymes under different N sources followed the order of NH₄NO₃ > NO₃⁻ > NH₄⁺ sources, indicating that Mo has more complementary effects towards nitrate nutrition than the sole ammonium source in winter wheat. Interestingly, under −Mo-deprived conditions, cultivar ‘97003’ recorded more pronounced alterations in Mo-dependent parameters than ‘97014’ cultivar. Moreover, Mo application increased the proteins, amino acids, ammonium, and nitrite contents while concomitantly decreasing the nitrate contents in the same order of NH₄NO₃ > NO₃⁻ > NH₄⁺ sources that coincides with the Mo-induced N enzymes activities and expressions. The findings of the present study indicated that Mo plays a key role in regulating the N metabolism enzymes and assimilatory products under all the three N sources; however, the extent of complementation exists in the order of NH₄NO₃ > NO₃⁻ > NH₄⁺ sources in winter wheat. In addition, it was revealed that mineral elements profiles were mainly affected by different N sources, while Mo application generally had no significant effects on the mineral elements contents in the winter wheat leaves under different N sources.