Subgroups but not for competitive athletes. This study was designed to compare the ventilatory threshold (VT) with the last positive (+/+), equivocal (+/−), and negative (−/−) stages of the TT for highly trained cyclists. Twelve men (26.5 ± 4.6 years, 71.9 ± 7.6 kg) consented and completed the study, as approved by the university institutional review board. A maximal graded exercise test was used to identify VT, maximal aerobic capacity ( JOURNAL/jscr/04.03/00124278-201504000-00005/math_5MM1/v/2021-02-09T094120Z/r/image-tiff max: 65.9 ± 6.9 ml·kg−1·min−1), and maximal heart rate (HRmax: 187.3 ± 11.3 b·min−1). On a separate visit, the TT was administered using the same protocol. Participants were asked if they could speak comfortably after a standard passage recitation. Response options were: “Yes” (+/+), “I'm not sure” (+/−), or “No” (−/−). Variables at VT were compared with the last (+/+), (+/−), and (−/−) stages of TT through t-test with Bonferroni's adjustment (0.05/3). Differences (p ≤ 0.017) were found between variables at VT, as compared with (+/+) TT ( JOURNAL/jscr/04.03/00124278-201504000-00005/math_5MM2/v/2021-02-09T094120Z/r/image-tiff : 32.9 ± 7.7 ml·kg−1·min−1, % JOURNAL/jscr/04.03/00124278-201504000-00005/math_5MM3/v/2021-02-09T094120Z/r/image-tiff : 49.9 ± 9.9, heart rate [HR]: 128.7 ± 18.7 b·min−1, %HRmax: 68.6 ± 7.9, rating of perceived exertion [RPE]: 11.1 ± 1.1) and (+/−) TT ( JOURNAL/jscr/04.03/00124278-201504000-00005/math_5MM4/v/2021-02-09T094120Z/r/image-tiff : 44.4 ± 7.5 ml·kg−1·min−1, % JOURNAL/jscr/04.03/00124278-201504000-00005/math_5MM5/v/2021-02-09T094120Z/r/image-tiff : 67.2 ± 7.5). There were no differences between RPE- and HR-based variables at VT, as compared with (+/−) TT (RPE: 13.6 ± 0.63, HR: 147.1 ± 17.2 b·min−1, %HRmax: 78.5 ± 7.4) or (−/−) TT ( JOURNAL/jscr/04.03/00124278-201504000-00005/math_5MM6/v/2021-02-09T094120Z/r/image-tiff : 48.8 ± 7.8 ml·kg−1·min−1, % JOURNAL/jscr/04.03/00124278-201504000-00005/math_5MM7/v/2021-02-09T094120Z/r/image-tiff : 73.9 ± 7.1, HR: 155.6 ± 13.6 b·min−1, %HRmax: 83.1 ± 5.3, RPE: 14.8 ± 0.90). We found that when the athlete could no longer speak comfortably, he was exercising at or near his VT; we concluded that (−/−) TT estimated VT and can therefore provide a practical method to gauge exercise intensity for highly trained competitive cyclists similar to those in our study....