Aboveground biomass (AGB) is a key indicator of forest structure and carbon sequestration, yet its dynamics under concurrent anthropogenic disturbances remain poorly understood. This study investigates the spatiotemporal dynamics and driving mechanisms of AGB in the Jianfengling tropical lowland rainforest (JFLTLR) within Hainan Tropical Rainforest National Park (NRHTR) from 2015 to 2023. Six machine learning models—Extreme Gradient Boosting (XGBoost), Gradient Boosting Machine (GBM), Support Vector Machine (SVM), k-Nearest Neighbors (KNN), Decision Tree (DT), and Random Forest (RF)—were evaluated, with RF achieving the highest accuracy (R² = 0.83). Therefore, RF was employed to generate high-resolution annual AGB maps based on Sentinel-1/2 data fusion, field surveys, socio-economic indicators, and topographic variables. Human pressure was quantified using the Human Influence Index (HII). Threshold analysis revealed a critical breakpoint at ΔHII ≈ 0.1712: below this level, AGB remained relatively stable, whereas beyond it, biomass declined sharply (≈−2.65 mg·ha⁻¹ per 0.01 ΔHII). Partial least squares structural equation modeling (PLS-SEM) identified plantation forests as the dominant negative driver, while GDP (−0.91) and road (−1.04) exerted strong indirect effects through HII, peaking in 2019 before weakening under ecological restoration policies. Spatially, biomass remained resilient within central core zones but declined in peripheral regions associated with road expansion. Temporally, AGB exhibited a trajectory of decline, partial recovery, and renewed loss, resulting in a net reduction of ≈ 0.0393 × 10⁶ mg. These findings underscore the urgent need for a “core stabilization–peripheral containment” strategy integrating disturbance early-warning systems, transportation planning that minimizes impacts on high-AGB corridors, and the strengthening of ecological corridors to maintain carbon-sink capacity and guide differentiated rainforest conservation.