Compounded medications (CMs) have become increasingly relevant in veterinary pharmacotherapy, particularly due to the limited availability of licensed veterinary medicinal products (VMPs). Despite the importance of CMs, data on their use all over the world, as well as in Serbian veterinary practice, remain limited. The aim of this study is to understand Serbian veterinarians’ attitudes towards the use of CMs and human medicinal products in small animal practice, with a focus on challenges and needs they experience related to this type of therapy. A questionnaire-based survey was conducted from January to May 2024 among licensed veterinarians, covering sociodemographics, antibiotic prescribing practices, and attitudes toward CMs. Most respondents (85.4%) reported difficulties in accessing appropriate medications, with 45.8% frequently prescribing human medications for reasons of availability and cost. While 58.3% were familiar with CMs, many emphasized their benefits in tailoring doses and formulations. A majority (65.6%) believed CMs could improve treatment outcomes, yet 87.5% expressed the need for further education. This study shows that Serbian veterinarians frequently prescribe human medications due to limited and costly veterinary medicinal products. While they see the benefits of CMs for personalized treatments, there is a clear need for improved training and clearer regulations to enhance treatment outcomes and ensure safer veterinary care.

Nanotechnology, based on nanoparticles, has become an emerging interdisciplinary tool in reproductive biotechnology, offering innovative opportunities to improve fertilization efficiency and reproductive performance in farm animals. The purpose of this review is to provide an updated synthesis of current research on nanoparticle-based approaches that enhance in vitro fertilization outcomes and other assisted reproductive technologies. The focus is on the biological mechanisms, potential benefits, and limitations of nanoparticle use in animal reproduction. Nanoparticles—including gold, silver, zinc oxide, selenium, and magnetic iron oxide—exhibit distinctive physicochemical properties that enable targeted interactions with gametes and reproductive cells. When used in semen extenders or culture media, nanoparticles improve sperm motility, acrosome and membrane integrity, and reduce oxidative stress and apoptosis. These effects contribute to enhanced fertilization rates and higher embryo developmental competence. In addition, nanoparticles can function as carriers for hormones, antioxidants, and growth factors, stabilizing reagents essential for oocyte maturation, sperm capacitation, and early embryo culture. The review also discusses nanopurification (selectively isolating and removing particles) and nanosorting (separating or organizing nanoscale objects) techniques that allow for non-invasive selection of viable gametes, and fluorescence- and magnet-assisted sorting systems that increase precision in sperm sexing. The mechanical aspects of nanoparticle–cell interactions are analyzed, emphasizing the influence of particle size, dose, and surface modification on both biological efficacy and cytotoxicity. Safety, toxicological concerns, and regulatory frameworks—including International Organization for Standardization (ISO) standards and European Commission recommendations—are critically reviewed to highlight the need for harmonized biocompatibility criteria. Although nanoparticle use in animal reproduction remains largely experimental, accumulated evidence demonstrates its potential to improve reproductive efficiency and reduce economic losses. Integrating nanoparticle-based systems with existing reproduction platforms may represent a transformative step toward sustainable and precision-driven livestock breeding.

The welfare of pet rabbits is frequently compromised despite increasing recognition of their complex behavioural and physiological needs. Although owner knowledge is considered an important determinant of animal care, the relationship between perceived knowledge and actual husbandry practices remains poorly understood. This study aimed to (i) describe husbandry practices and owner-reported knowledge among pet rabbit owners in Croatia and (ii) assess whether higher perceived knowledge is associated with greater alignment with recommended husbandry practices. Data were collected using an online questionnaire distributed to rabbit owners (n = 529). The study assessed owner-reported husbandry practices and perceived knowledge but did not include direct assessments of rabbit health or welfare. Although most respondents reported high levels of knowledge regarding rabbit care (85.6%), this was not consistently reflected in implemented husbandry practices. Higher perceived knowledge was significantly associated (p < 0.05) with environmental enrichment, housing type, and cleaning frequency, but these associations were generally weak to moderate. In contrast, perceived knowledge showed little or no association with several key welfare-related practices, including social housing and access to free movement, while only limited associations were observed for feeding practices. These findings reveal a substantial knowledge–practice gap, suggesting that confidence in rabbit care knowledge does not necessarily translate into appropriate husbandry. Therefore, educational approaches focused solely on information provision may be insufficient to ensure the implementation of recommended husbandry practices.

The aim of this study was to improve surgical caponisation and identify stress factors associated with different pain management methods and post-operative periods. A total of 72 Prelux-G cockerels were caponised at six weeks of age. Eight experimental groups were established based on caponisation status and pain management. Four groups were caponised using different pain management protocols (none, anaesthesia only, analgesia only, or both), while four non-caponised groups served as controls, including one group that received anaesthesia and analgesia without surgery. The remaining three groups served as non-caponised controls with varying levels of handling. The researchers monitored body weight, feed intake, respiratory and heart rate, vocalisations, response to pain, and depth of anaesthesia. The results confirmed that caponisation is painful, as reflected in increased physiological responses and vocalisations. The use of non-steroidal anti-inflammatory drugs alone did not provide effective pain relief. Additionally, the surgical procedure had no significant effect on weight gain or feed intake during the seven days following surgery. Stress levels, measured by blood serum corticosterone concentration, also increased during the procedure. The study concludes that anaesthesia significantly reduces pain and facilitates surgery, which is essential for improving animal welfare.