Atrazine (ATZ) is a herbicide used in agricultural production and has been detected in surface water due to its widespread use worldwide. This may pose a threat to the health of aquatic animals. To explore the ATZ−induced hepatic metabolism disorder, male zebrafish were exposed to 300 and 1000 μg/L ATZ for 21 days, respectively. The results revealed that ATZ exposure significantly reduced hepatic triglyceride (TG) levels, while significantly (p < 0.05) increased pyruvate (PYR) and total cholesterol (TC) levels. In addition, the liver sample from the 1000 μg/L ATZ−treated group was used for GC/MS metabolomic analysis. The principal component analysis (PCA) model showed significant separation of the 1000 μg/L ATZ group from the control group, indicating that ATZ exposure altered hepatic metabolism in male adult zebrafish. A total of 29 significantly (p < 0.05) different metabolites were observed and identified in the ATZ−treated group. Moreover, the most disturbed pathways by ATZ were the arginine and proline metabolic pathways, followed by the glutathione metabolic pathway. Three and two metabolites were significantly altered in the arginine and proline metabolic pathways and glutathione metabolic pathway, respectively. Based on these results, we suggested that ATZ was capable of altering liver metabolism in zebrafish and that its ecological risk to aquatic organisms cannot be ignored.

Carbendazim is usually used to control the Sclerotinia sclerotiorum of rapes during the flowering period. This paper presents a study on transfer assessment of carbendazim residues from rape flowers to apicultural products. In the field trials, the rapes were sprayed with carbendazim on standard dosage. Bees produced apicultural products (bee pollen, honey, and royal jelly) from sprayed rapes. Apicultural products were collected on a regular basis. Carbendazim residues were extracted from bee pollen, honey, and royal jelly, respectively. HPLC/ESI-MS/MS method was developed and partially validated to identify and quantify carbendazim residues. The limits of quantification in pollen, honey, and royal jelly were 0.01 mg/kg. Mathematical curve fitting was carried out on the basis of transfer assessment of carbendazim residues from rape flowers to apicultural products. The respective carbendazim residues were 1.10±0.03 mg/kg in pollen on 18th day, 0.032±0.001 mg/kg in honey on 24th day, and 0.077±0.002 mg/kg in royal jelly on 22nd day. Transfer assessment and mathematical curve fitting of carbendazim residues from rape flowers to apicultural products show carbendazim diminished over spraying time. The gap of carbendazim residues between pollen and honey is decreased with time. The carbendazim residues in pollen are 10 times higher than that of honey and jelly.