The corn earworm, Helicoverpa zea (Boddie), is a common lepidopteran pest of corn (Zea mays L.) in the United States. This article provides an overview of the life history, ecology, plant injury, and management of H. zea in corn. Leaf injury by H. zea feeding can occur in vegetative stage corn, though this type of injury is rare. The most common type of injury is caused by larval feeding in ears. Because kernel injury is typically limited to the tip of the ear, H. zea is generally not considered an economic pest. The use of transgenic corn hybrids expressing insecticidal Cry proteins from Bacillus thuringiensis (Bt) Berliner can reduce injury from H. zea, though complete control is not achieved with most Bt traits. Resistance has been reported to several Bt toxins. Because H. zea is a major economic pest in cotton (Gossypium hirsutum L.) and because H. zea moths that developed on corn can migrate to cotton, selection pressure on H. zea in Bt corn has major implications for the pest status and injury to Bt cotton. Although the impact of H. zea on yield of field corn is generally negligible, the selection pressure exerted by Bt corn has led to management issues in cotton.

The corn earworm, Helicoverpa zea (Boddie), is managed in corn and cotton in the United States primarily using transgenic cultivars that produce insecticidal proteins from the bacterium Bacillus thuringiensis (Bt). However, increasing reports of resistance to one or more Bt proteins threaten the continued efficacy of Bt traits. To better understand the development of resistance of H. zea to Bt corn and cotton in the southeastern United States, we monitored for resistance to Cry1A.105 and Cry2Ab2 among 22 field populations of H. zea collected in non-Bt and Bt corn expressing Cry1A.105 + Cry2Ab2 during 2017 and 2018. Colonies were established in the laboratory and progeny were screened in diet-overlay bioassays to purified Cry1A.105 and Cry2Ab2 proteins. Compared with two susceptible laboratory colonies, all 14 field colonies tested with Cry1A.105 were highly resistant, with resistance ratios (RRs) ranging from 13.5 to >4,000. For Cry2Ab2, 19 colonies were tested and RRs ranged from 0.26 to 33.7. Field populations were significantly more susceptible to Cry2Ab2 than Cry1A.105. We documented variability in F0 and F1 pupal weight and developmental rates of natural populations of H. zea, but observed no significant correlation with susceptibility to either Cry1A.105 or Cry2Ab2. Our results expand on the recent reports of H. zea resistance to Cry1A and Cry2A proteins and will aid in the design and deployment of future pyramided crops in the United States.

As part of an insect resistance management plan to preserve Bt transgenic technology, annual monitoring of target pests is mandated to detect susceptibility changes to Bt toxins. Currently Helicoverpa zea (Boddie) monitoring involves investigating unexpected injury in Bt crop fields and collecting larvae from non-Bt host plants for laboratory diet bioassays to determine mortality responses to diagnostic concentrations of Bt toxins. To date, this monitoring approach has not detected any significant change from the known range of baseline susceptibility to Bt toxins, yet practical field-evolved resistance in H. zea populations and numerous occurrences of unexpected injury occur in Bt crops. In this study, we implemented a network of 73 sentinel sweet corn trials, spanning 16 U.S. states and 4 Canadian provinces, for monitoring changes in H. zea susceptibility to Cry and Vip3A toxins by measuring differences in ear damage and larval infestations between isogenic pairs of non-Bt and Bt hybrids over three years. This approach can monitor susceptibility changes and regional differences in other ear-feeding lepidopteran pests. Temporal changes in the field efficacy of each toxin were evidenced by comparing our current results with earlier published studies, including baseline data for each Bt trait when first commercialized. Changes in amount of ear damage showed significant increases in H. zea resistance to Cry toxins and possibly lower susceptibility to Vip3a. Our findings demonstrate that the sentinel plot approach as an in-field screen can effectively monitor phenotypic resistance and document field-evolved resistance in target pest populations, improving resistance monitoring for Bt crops.

Sugarcane aphid, Melanaphis sacchari Zehtner (Hemiptera: Aphididae), outbreaks on grain sorghum were first detected in the United States in 2013. The spread of sugarcane aphid across the sorghum-producing regions of North America necessitated increased insecticide use to mitigate economic loss. A field experiment to develop economic thresholds for sugarcane aphid was conducted 15 times across seven locations across the southern United States during 3 yr (2014-2016). Grain sorghum hybrids were evaluated by measuring yield in response to a range of aphid infestations. Yield-aphid population density relationships were described by linear function, which facilitated calculating economic injury levels and economic thresholds. The slopes of the yield-aphid density regressions were significant, negative, and relatively stable across locations, years, and agronomic conditions. The relationships aggregated into two groups, populations that exhibited relatively slow and fast population growth, and common economic injury levels were determined using control costs and market values of grain. Average economic injury levels of 37 and 102 aphids per leaf were most applicable to the two groupings of sorghum/aphid relationships and aphid population growth. Using field-based sugarcane aphid population doubling time estimated from weekly observations of aphid densities, economic thresholds were calculated, ranging from 19 to 132 aphids per leaf across the 15 locations-years. Without site-specific knowledge of a slow-growing aphid population and given cost and market price variability of the system, a 40 aphid per leaf threshold is most prudent to use across the range of hybrid, environmental, and market conditions experienced in this study.

Transgenic corn, Zea mays L., hybrids expressing insecticidal Cry proteins from Bacillus thuringiensis (Bt) and insecticide applications to suppress

A 2-yr study was conducted in South Carolina wheat fields to describe spatial and temporal dynamics of stink bugs sampled with sweep nets. In 2008, t