Rational cropping maintains high soil fertility and a healthy ecosystem. Soil microorganism is the controller of soil fertility. Meanwhile, soil microbial communities also respond to different cropping patterns. The mechanisms by which biotic and abiotic factors were affected by different cropping sequences remain unclear in the major grain-producing regions of northeastern China. To evaluate the effects of different cropping sequences under conventional fertilization practices on soil properties, microbial communities, and crop yield, six types of plant cropping systems were performed, including soybean monoculture, wheat-soybean rotation, wheat-maize-soybean rotation, soybean-maize-maize rotation, maize-soybean-soybean rotation and maize monoculture. Our results showed that compared with the single cropping system, soybean and maize crop rotation in different combinations or sequences can increase soil total organic carbon and nutrients, and promote soybean and maize yield, especially using soybean-maize-maize and maize-soybean-soybean planting system. The 16S rRNA and internal transcribed spacer (ITS) amplicon sequencing showed that different cropping systems had different effects on bacterial and fungal communities. The bacterial and fungal communities of soybean monoculture were less diverse when compared to the other crop rotation planting system. Among the different cropping sequences, the number of observed bacterial species was greater in soybean-maize-maize planting setup and fungal species in maize-soybean-soybean planting setup. Some dominant and functional bacterial and fungal taxa in the rotation soils were observed. Network-based analysis suggests that bacterial phyla Acidobacteria and Actinobacteria while fungal phylum Ascomycota showed a positive correlation with other microbial communities. The phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) result showed the presence of various metabolic pathways. Besides, the soybean-maize-maize significantly increased the proportion of some beneficial microorganisms in the soil and reduced the soil-borne animal and plant pathogens. These results warrant further investigation into the mechanisms driving responses of beneficial microbial communities and their capacity on improving soil fertility during legume cropping. The present study extends our understanding of how different crop rotations effect soil parameters, microbial diversity, and metabolic functions, and reveals the importance of crop rotation sequences. These findings could be used to guide decision-making from the microbial perspective for annual crop planting and soil management approaches.

We investigated the beneficial role of different concentrations of exogenous oxalic acid (OA) or citric acid (CA) for improving Pb tolerance and mitigating Pb-induced physiological toxicity in Changbai larch (Larix olgensis A. Henry) seedlings in northeast China. The seedlings were exposed to 100 mg·kg⁻¹ Pb in soil alone or in combination with OA or CA irrigation for 10, 20, or 30 days. Pb-induced damage in L. olgensis was evident from elevated lipid peroxidation that significantly inhibited plant growth. Malondialdehyde (MDA) contents also increased in the presence of elevated Pb; however, superoxide dismutase (SOD) and peroxidase (POD) activities, as well as proline and pigment contents, all decreased. The damage increased in controls over the application periods. Pb contents in fine roots and leaves generally decreased with low-concentration organic acids (<1.0 mmol·L⁻¹), but often increased at 5.0 and 10.0 mmol·L⁻¹. Alternatively, when Pb-stressed plants were exposed to an organic acid (especially 5.0 or 10.0 mmol·L⁻¹ for 10 days), the damage, as indicated by the physiological parameters, was reversed, and plant growth was promoted; CA was more effective in inducing these changes than OA. Therefore, exogenous organic acids have the potential to alleviate Pb-induced oxidative injuries, and can improve the tolerance of L. olgensis seedlings to Pb stress. Under lower OA and CA concentrations, the detoxification mechanism appears to be an external resistance mechanism; however, under higher concentrations (5.0–10.0 mmol·L⁻¹) internal resistance mechanisms appear dominant. It is also possible that the two mechanisms work in tandem.

We investigated the impacts of graphene application at different concentrations on the growth and physiological characteristics of Changbai larch (Larix olgensis A. Henry) seedlings and the chemical properties and enzyme activities of Haplic Cambisols under these seedlings. The aim is to evaluate the environmental effects of graphene on the afforestation species and the zonal forest soils of Northeast China. Seedlings receiving 0 (CK), 25, 50, 100, 250, or 500 mg L⁻¹ graphene were incubated for 30, 40, or 50 days. Low concentrations (25–50 mg L⁻¹) of graphene increased the dry masses of root, stem, and leaf; however, high concentrations (100–500 mg L⁻¹) inhibited them. Compared with those under 0 mg L⁻¹ graphene, the root length, surface area, volume, and average diameter all increased during the early stages of incubation (i.e., 30 and 40 days) under low concentration of graphene (100 mg L⁻¹); at 50 days, they were significantly inhibited. At 30 days, graphene decreased superoxide dismutase (SOD) and peroxidase (POD) activities, as well as pigment, soluble protein, and proline contents, and the decline increased with increasing graphene concentration; at 40 and 50 days, the above parameters increased initially and then decreased, reaching a maximum at 50 mg L⁻¹. The changes in relative conductivity and malondialdehyde (MDA), superoxide anion and hydrogen peroxide contents were the opposite of those in the physiological indexes mentioned above. Therefore, graphene caused different degrees of oxidative stress in L. olgensis seedlings. At 30 days, graphene generally increased the organic matter, hydrolytic nitrogen, and available phosphorus and potassium contents of Haplic Cambisols, but these parameters decreased at 40 and 50 days. Graphene generally decreased acid phosphatase, urease, dehydrogenase, and catalase activities. Therefore, when graphene reaches a certain content level in this soil, it may also affect nitrogen and phosphorus cycling.