Introduction: obesity is a major public health issue, linked to chronic stress and metabolic dysregulation. While biological mechanisms of sedentary behavior in obesity are well known, epigenetic factors, particularly those involving the NR3C1 gene, remain unclear, especially in genes related to the regulation of chronic stress and metabolism, such as NR3C1. Objective: this study aimed to investigate the methylation profile of the NR3C1 gene in obese individuals with physical inactivity. Methods: this cross-sectional study included 119 adult volunteers with obesity (BMI ≥30) who accessed public primary health care services. Individuals using glucocorticoids were excluded. Socioeconomic, health, and lifestyle data were collected using structured questionnaires, and depressive symptoms were assessed using the Beck Depression Inventory (BDI-II). Peripheral blood samples were collected in the morning for cortisol quantification and molecular analysis of the NR3C1 gene via pyrosequencing. Multivariate Poisson regression was applied to identify factors associated with physical inactivity. Results: poisson multivariate regression analysis with robust variance showed that physical inactivity was associated with NR3C1 gene hypermethylation at CpGs 44, 45, and 46 in obese individuals and showed that physical inactivity was associated with low cortisol in the obese population. Conclusion: this study suggests an association between a sedentary lifestyle and changes in NR3C1 gene methylation in obese individuals. The association between physical inactivity and low cortisol levels strengthens the hypothesis that a sedentary lifestyle may involve epigenetic action in the dysregulation of the HPA axis of stress adaptation in obese individuals.

Esse estudo buscou verificar a ação da própolis vermelha como revestimento comestível na conservação da maçã da cultivar “Fuji”, utilizando temperaturas de 7°C (refrigerada) e 30° (ambiente) e nas concentrações de própolis a 3% e 5%, para a pesquisa, foi utilizado essa fruta por seu valor nutricional, rica pectina, tanino e flavonóides, que são fitonutrientes, que agem como antioxidantes e previnem diversas doenças. A solução filmogênica aplicada foi extrato de própolis vermelha, que possui substâncias que retardam o amadurecimento da cultivar, assim contribuindo para o aumento da sua vida útil. As amostras foram imergidas nas concentrações, em seguida postas em seus devidos ambientes, de onde eram retiradas a cada 5 dias, durante 30 dias, foram realizadas analises microbiológicas, que constatom a ausência de Salmonela ssp e Staphylococcus spp, e observou-se o retardo do desenvolvimento de fungos filamentosos, coliformes 35° e coliformes 45° até o 15° dia para a concentração a 3%, e 25 dias para a 5%, confirmando a eficiência do revestimento contra o desenvolvimento de Microorganismo, Em relação a físico-química, as amostras apresentaram uma interação no ratio, contribuindo para o estado de maturação do fruto.

As palmas forrageiras do gênero Opuntia (Cactacea) são originadas do México e consideradas as principais fontes de forragem para rebanhos na Região Nordeste do Brasil. O aprimoramento genético aumenta consideravelmente a produção da palma, ampliando sua adaptação, surgindo novos cultivares, corroborando, assim, com os avanços nas pesquisas da palma forrageira nas regiões semiáridas. A alta produtividade, o melhoramento genético da palma também enfrenta o desafio de resistirem à cochonilha do carmim Dactylopius coccus Costa, 1835 (Hemiptera: Dactylopiidae). As principais pragas que atacam as palmas podem ser efetivamente controladas geneticamente. O propósito de avaliar os métodos de melhoramento genético, seus resultados e adaptada ao semiárido, essa pesquisa bibliográfica teve como principal objetivo mostrar, com base na literatura pertinente, o potencial de adaptação destas plantas às regiões semiáridas viabilizando um estudo técnico de principais espécies de palma forrageira com potencialidades de resistência à cochonilha do carmim.

Viral infections represent a critical threat to cultivated plant species. In papaya cultivation, two viral diseases—papaya mosaic (caused by papaya ringspot virus type P—PRSV-P) and papaya sticky disease (caused by a virus complex of papaya meleira virus—PMeV, and papaya meleira virus—PMeV2)—are prevalent and capable of devastating entire plantations, incurring substantial economic losses. Current diagnostic practices rely on visual identification of symptoms and elimination of infected plants (roguing). Monitoring photosynthetic efficiency in orchards prone to PRSV-P and PMeV2 coinfection may allow early intervention, mitigating productivity losses and reducing fruit quality. This study aimed to evaluate chlorophyll a fluorescence as a biomarker for photosynthetic impairment and symptom severity in papaya infected with PRSV-P and/or PMeV2 and to explore the feasibility of early detection of the infection by these dual pathogens, as an exploratory study under field conditions. Chlorophyll a fluorescence revealed details about the physiology of plants coinfected with the complex of PMeV2 and PRSV-P: the electron motive force within PSII decreases in infected plants and in those without visual symptoms of infection, being proportional to the age and developmental stage of the plants. A slowdown in the multiple electron transfer turnover of PSII and a decrease in the efficiency of the redox reactions of photosystem I were observed in plants with or without visual detection of infection. The evidence generated suggests that the chlorophyll a fluorescence technique can be used to monitor the pathophysiological state of plants under biotic stress.

Propolis is a natural resin made by bees of the genus Apis sp., And because of its antioxidant and antimicrobial activity, it has been used frequently in alternative medicine in several places of the world. These biological characteristics are related to their chemical composition, precisely to the phenolic compounds that vary in relation to their structure and concentration depending on the region of production, availability of plant sources for resin collection and time of year. Several experiments have been conducted in order to define the compounds that characterize these activities. The aim of this study was to elaborate and identify the chemical composition of the hydroalcoholic extracts of red, green and black propolis by High Performance Liquid Chromatography. The profiles presented by High Efficiency Liquid Chromatography (HPLC) of the extracts of the green, red and black propolis samples were very similar, but there was a great variation in terms of quantitative profile, besides playing an essential role in the antioxidant activity. Through the chromatograms, it is verified that as a function of the concentration of propolis, different phenolic compounds are extracted from the propolis, resulting in extracts with different biological properties. These data contribute to the knowledge of the biological activity of the hydroalcoholic extract of green propolis, favoring the search for new therapeutic agents.

O artigo "Dinâmicas Sociais e Produtivas no Contexto Rural: A Importância das Mulheres Rurais na Paraíba e a Agroecologia" investiga as dinâmicas sociais e produtivas no meio rural, com ênfase na contribuição das mulheres rurais da Paraíba. A pesquisa é motivada pela necessidade de compreender as desigualdades de gênero presentes no sistema capitalista, onde a divisão sexual do trabalho marginaliza as mulheres. O estudo objetiva analisar o papel das mulheres na agricultura familiar e na transição energética para energias renováveis. Para a metodologia do trabalho foi realizada uma observação participante durante a 15ª Marcha pela Vida das Mulheres e pela Agroecologia, em Areial - PB, que destacou a importância da Caatinga no enfrentamento das mudanças climáticas. Os resultados indicam que, apesar de enfrentarem uma tripla jornada de trabalho, as mulheres rurais desempenham um papel crucial na sustentabilidade agrícola e na resistência aos modelos centralizados de geração de energia. A análise revela a necessidade de políticas que promovam a igualdade de gênero e o reconhecimento do trabalho das mulheres no meio rural.