Increasing yield and resistance to pathogens are important objectives in plant breeding. However, difficulties in breeding are encountered due to the antagonistic relationship between crop yield production and immunity pathways (Ning et al., 2017). Planting density and plant architecture are key factors determining crop yields in a given area. The plant traits of tiller and lamina joint angles have long attracted the attention of breeders due their significant contributions to plant architecture by enhancing photosynthetic efficiency and facilitating enhanced planting density (Sakamoto et al., 2006; Wang and Li, 2008).

Ammonium transporter (AMT) proteins have been reported in many plants, but no comprehensive analysis was performed in wheat. In this study, we identified 23 AMT members (hereafter TaAMTs) using a protein homology search in wheat genome. Tissue specific expression analysis showed that TaAMT1;1a, TaAMT1;1b, and TaAMT1;3a were relatively more highly expressed in comparison with other TaAMTs. TaAMT1;1a, TaAMT1;1b, and TaAMT1;3a-GFP were localized in the plasma membrane in tobacco leaves, and TaAMT1;1a, TaAMT1;1b, and TaAMT1;3a successfully complemented a yeast 31019b strain in which ammonium uptake was deficient. In addition, the expression of TaAMT1;1b in an Arabidopsis AMT quadruple mutant (qko) successfully restored NH4+ uptake ability. Resupply of NH4+ rapidly increased cellular NH4+ contents and suppressed expression of TaAMT1;3a, but not of TaAMT;1;1a and TaAMT1;1b expressions. Expression of TaAMT1;1a, TaAMT1;1b, and TaAMT1;3a was not changed in leaves after NH4+ resupply. In contrast, N deprivation induced TaAMT1;1a, TaAMT1;1b, and TaAMT1;3a gene expressions in the roots and leaves. Expression analysis in the leaves of the stem rust-susceptible wheat line “Little Club” and the rust-tolerant strain “Mini 2761” revealed that TaAMT1;1a, TaAMT1;1b, and TaAMT1;3a were specifically induced in the former but not in the latter. Rust-susceptible wheat plants grown under N free conditions exhibited a lower disease index than plants grown with NH4+ as the sole nitrogen source in the medium after infection with Puccinia graminis f. sp. tritici, suggesting that NH4+ and its transport may facilitate the infection of wheat stem rust disease. Our findings may be important for understanding the potential function TaAMTs in wheat plants.

Rhizoctonia solani causes sheath blight disease in rice; however, the defense mechanism of rice plants against R. solani remains elusive. To analyze the roles of brassinosteroid (BR) and ethylene signaling on rice defense to R. solani, wild-type (WT) rice and several mutants and overexpressing (OX) lines were inoculated with R. solani. Mutants d61-1 and d2 were less susceptible to sheath blight disease, bri1-D was more susceptible, and ravl1 and d61-1/EIL1 Ri5 were similarly susceptible compared with WT. The double mutant ravl1/d61-1 was phenotypically similar to the ravl1 mutant. Transcriptome analysis, chromatin immunoprecipitation assay, electrophoretic mobility shift assay, and transient assays indicted that RAVL1 might directly activate Ethylene insensitive 3-like 1 (EIL1), a master regulator of ethylene signaling. Mutants ers1 and d61-1/RAVL1 OX were resistant to sheath blight disease, whereas EIL1 RNAi mutants and RAVL1 OX were more susceptible than WT. BRI1 and D2 expression in EIL1 Ri5/RAVL1 OX and EIL1 expression in d61-1/RAVL1 OX indicated that RAVL1 activates BRI1/D2 and EIL1, respectively, independent of BR and ethylene signaling. Our analyses provide information on how BR and ethylene signaling regulate sheath blight disease and on the regulatory function of RAVL1 in rice sheath blight disease.

Wheat powdery mildew, caused by the biotrophic fungus Blumeria graminis f. sp. tritici (Bgt), is a serious disease of wheat worldwide that can cause significant yield losses. Growing resistant cultivars is the most cost-effective and eco-soundly strategy to manage the disease. Therefore, a high breeding priority is to identify genes that can be readily used either singly or in combination for effective resistance to powdery mildew and also in combination with genes for resistance to other diseases. Yunnan Province, with complex and diverse ecological environments and climates, is one of the main wheat growing regions in China. This region provides initial inoculum for starting epidemics of wheat powdery mildew in the region and other regions and thus, plays a key role in the regional and large-scale epidemics of the disease throughout China. The objectives of this study were to evaluate seedling resistance of 69 main wheat cultivars to powdery mildew and to determine the presence of resistance genes Pm3, Pm8, Pm13, Pm16, and Pm21in these cultivars using gene specific DNA markers. Evaluation of 69 wheat cultivars with six Bgt isolates showed that only four cultivars were resistant to all tested isolates, indicating that the overall level of powdery mildew resistance of Yunnan wheat cultivars is inadequate. The molecular marker results showed that 27 cultivars likely have at least one of these genes. Six cultivars were found likely to have Pm3,18 likely to have Pm8,5 likely to have Pm16,and 3 likely to have Pm21. No cultivar was found to carry Pm13. The information on the presence of the Pmresistance genes in Yunnan wheat cultivars can be used in future wheat disease breeding programs. In particular, cultivars carrying Pm21, which is effective against all Bgtraces in China, should be pyramided with other effective genes to developing new cultivars with durable resistance to powdery mildew.

Brassinosteroids (BRs) are a group of bioactive steroid molecules that act as plant hormones. Brassinosteroid signaling is monitored by the plasma membrane (PM)‐bound receptor BRASSINOSTEROID INSENSITIVE1 (BRI1), a leucine‐rich repeat (LRR) receptor‐like kinase (RLK) (Belkhadir & Jaillais, 2015). Binding of BRs to the extracellular domain of BRI1 initiates an intracellular signal cascade response which is rapidly transmitted to the nucleus ultimately resulting in transcription of BR‐responsive genes (Jaillais & Vert, 2016). Brassinosteroid responsive genes ultimately help to control various aspects of plant growth and development, including root development, cell division, vascular patterning, organ boundary development, stress response and pathogen defense (Nakashita et al., 2003; Yamamoto et al., 2007; Gonzalez‐Garcia et al., 2011; Gendron et al., 2012; Bekh‐Ochir et al., 2013).