Background: There is a continuous research in the area of biomimetic coatings on the titanium (Ti) implant surfaces for improved survival and long-term successful outcomes in the field of dentistry and orthopedics. In-vitro approaches are ideal systems for studying cell-material interactions without complexity and interference observed in in-vivo models.Purpose: The present study was undertaken to evaluate the osteoblast characteristics and function on Ti substrates coated with the novel composite coating of ceramic apatite-wollastonite (AW) and polymer chitosan.Materials and methods: Ti substrate coated with composite AW-Chitosan was synthesized, using electrophoretic deposition. MG-63 cells were seeded onto the coated substrates and cellular morphology and growth was assessed using Scanning Electron Microscopy (SEM) and Laser Scanning Microscopy (LSM). Osteocalcin expression of the seeded cells was assessed by FITC tagging and LSM analysis. Alizarin Red S staining and Confocal LSM (CSLM) analysis was used to study the in-vitro mineralization on the titanium samples.Results: The AW-Chitosan coating on Ti samples by electrophoretic deposition exerted significant positive influence on cell proliferation, growth and mineralization as compared to uncoated titanium samples. Scanning electron microscopy and laser confocal microscopy experiments revealed that the coating was non-toxic to cells, enhanced adhesion and proliferation of MG-63 cells. Increased functional activity was observed by increased production of bone-specific protein osteocalcin and mineralized calcium through day 7 and 14.Conclusions: The present study underscores that optimal inorganic-organic phase nanocomposite crack-free coating created on Ti by simple, cost-effective electrophoretic deposition technique may have osteoconductive potential and may have wide application in the field of implantology.

Background: A fracture of denture base in situ often occurs through a fatigue mechanism, which over a period of time leads to the formation of small cracks, resulting in fracture. Aim and Objective: To evaluate the flexural strength of repaired heat-polymerized acrylic resin, with different percentage of aluminum oxide (Al₂O₃) added to the repair resin and effect of two different surface treatments on the flexural strength of repaired heat-polymerized acrylic resin and also to evaluate quantification of filler particles using scanning electron microscopy. Materials and Methodology: Fifty specimens of heat-polymerized acrylic resin were prepared according to the American Dental Association specification no. 12 (65 mm × 10 mm × 2.5 mm). Al₂O₃<50 nm particle size was silanized using metal alloy primer before incorporation in polymer. Two different percentages of Al₂O₃nanoparticles, that is, 1% and 1.5% were added to autopolymerizing acrylic resin which was used as repairing material. Results: The study showed that repair resin incorporated with 1.5% Al₂O₃in the group surface treated with silicon carbide paper improved the flexural strength of denture base resin. A proper filler distribution and deep penetration within the polymer matrix were observed by scanning electron microscope in the same group.