The aim of the current study was to evaluate the possibility of application of bioelectrical impedance analysis (BIA) in order to estimate pork quality. The BIA measurements were tested on 18 living animals for the prediction of the meat quality. The absolute resultant electrical resistance (Rz) and reactance (Xc) of the body was measured with a set of disposable surface electrodes at the frequency of 50 kHz and the current intensity of 400 µA. The characteristics of meat quality, pH measured 1 h and 24 h after slaughter, meat color parameters represented in CIE L*a*b* system, glycolytic potential, intramuscular fat, and natural drip loss, were assessed on the samples of the Longissimus dorsi (LD) muscle. The slaughter value of pigs was characterized on the basis of hot carcass weight (HCW) and percent of meat in carcass. The results showed a significant Pearson correlation between bioelectrical impedance parameter Rz and pH1 (r = 0.48*, p < 0.05). A significant Spearman correlation was showed between color b* value and the Rz/Xc/HCW ratio (r = −0.62*, p < 0.05) and Xc (r = −0.51*, p < 0.05), as well as between the Rz/Xc ratio with pH1 (r = 0.48*, p < 0.05). The multivariate statistical method (principal component analysis and cluster analysis) showed that bioimpedance measurements combined with meat quality traits make it possible to distinguish groups with different quality parameters. However, the relationships between them are complex and still require analysis.
The FTO gene rs9939609 has been positively associated with obesity in humans, but its role in dietary intake is still unclear. The aim of this study was to investigate the relationship between nutritional status and dietary intake in adults and the role of the FTO gene polymorphism in this context. In a group of 112 adults (74% women, mean age 40.1 ± 13.24 years), body composition was analysed using the bioimpedance method and body weight status was assessed using BMI criteria. Diet was assessed by three-day food records and a food frequency questionnaire. The polymorphisms of the FTO rs9939609: AA, AT, and TT were analysed by fast real-time PCR in DNA isolated from buccal swabs. The data were statistically analyzed by using analysis of variance, non-parametric tests, and multivariate analyses (principal component analysis, correspondence analysis). The frequency of the mutant A allele in the study group was 0.46. The frequency analysis of individual genotypes (TT=0.26; AT=0.56; AA=0.18) revealed that the genotype distribution in the study group was consistent with the Hardy-Weinberg law. The rs9939609 FTO gene polymorphism was associated with frequency of excessive body weight. There was no difference in the values obtained from anthropometric measurements and body composition analysis in the group. The results showed that the studied polymorphism FTO gene explained only 7.82% of the variability in features characterising the nutritional status. The analysis of obesity prevalence within families revealed a significant impact from relatives on the female line.