Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis characterized by improved water use efficiency mediated by major nocturnal CO₂ fixation. Due to its inherent metabolic plasticity CAM represents a successful physiological strategy for plant adaptation to abiotic stress. The present study reports on the impact of drought stress and different light intensities (PPFD 50 and 200 μmol m^–2 s^–1) on the photosynthetic performance of the obligate CAM orchid Phalaenopsis “Edessa” by integrating diel gas exchange patterns with assessments of the light reactions by analyzing fast chlorophyll a fluorescence induction. Parameters such as PI_abs (performance index), different energy fluxes per active reaction centre (RC) reflecting the electron flow from photosystem II to photosystem I and the energetic communication between PSII complexes defined as connectivity were considered for the first time in a CAM plant. A higher PS II connectivity for plants grown under low light (p ∼ 0.51) compared to plants grown under high light (p ∼ 0.31) brought about similar specific energy fluxes of light absorbance, dissipation and processing through the electron transport chain, irrespective of the light treatment. With a 25% higher maximum quantum yield and comparable biomass formation, low light grown plants indeed proved to process light energy more efficiently compared to high light grown plants. The performance index was identified as a very reliable and sensitive parameter to indicate the onset and progress of drought stress. Under restricted CO₂ availability (due to closed stomata) leaves showed higher energy dissipation and partial inactivation of PSII reaction centres to reduce the energy input to the electron transport chain and as such aid in avoiding overexcitation and photodamage. Especially during CAM idling there is a discrepancy between continuous input of light energy but severely reduced availability of both water and CO₂, which represents the ultimate electron acceptor. Taken together, our results show a unique flexibility of CAM plants to optimize the light reactions under different environmental conditions in a dual way by either attenuating or increasing energy flux.

Temporal compartmentation of carboxylation processes is a defining feature of crassulacean acid metabolism and involves circadian control of key metabolic and transport steps that regulate the supply and demand for carbon over a 24h cycle. Recent insights on the molecular workings of the circadian clock and its connection with environmental inputs raise new questions on the importance of light quality and, by analogy, certain photoreceptors for synchronizing the metabolic components of CAM. The present work tested the hypothesis that optimal coupling of stomatal conductance, net CO2 uptake, and the reciprocal turnover of carbohydrates and organic acids over the diel CAM cycle requires both blue and red light input signals. Contrasting monochromatic wavelengths of blue, green, and red light (i.e. 475, 530, 630nm) with low fluence rates (10 μmol m(-2) s(-1)) were administered for 16 hours each diel cycle for a total treatment time of 48 hours to the obligate CAM bromeliad, Aechmea 'Maya'. Of the light treatments imposed, low-fluence blue light was a key determinant in regulating stomatal responses, organic acid mobilization from the vacuole, and daytime decarboxylation. However, the reciprocal relationship between starch and organic acid turnover that is typical for CAM was uncoupled under low-fluence blue light. Under low-fluence red or green light, the diel turnover of storage carbohydrates was orchestrated in line with the requirements of CAM, but a consistent delay in acid consumption at dawn compared with plants under white or low-fluence blue light was noted. Consistent with the acknowledged influences of both red and blue light as input signals for the circadian clock, the data stress the importance of both red and blue-light signalling pathways for synchronizing the metabolic and physiological components of CAM over the day/night cycle.

Abiotic stress is among the most critical factors limiting crop productivity worldwide and its importance is further exacerbated by climate change. In recent years, microalgal biostimulants have gained attention for their potential to enhance plant resilience towards abiotic stress. However, significant hurdles still persist, particularly regarding the unknown modes of action of microalgal biostimulants, which is a concern for stringent regulatory requirements and product reliability. The aim of this review is to improve the potential of microalgal biostimulants for abiotic stress mitigation in plants by addressing different key parameters shaping the efficacy of microalgal biostimulants, encompassing cultivation approaches, extraction techniques, and application methods. Furthermore, it also highlights how microalgal biostimulants modulate plant morphology, physiology and biochemistry under drought, salinity, and heat stress—three predominant stressors anticipated to intensify under climate change. Notably, these biostimulants consistently enhance drought stress tolerance by improving biomass accumulation, nutrient uptake, and water use efficiency through enhanced photosynthesis and stomatal regulation. These effects are largely driven by the accumulation of osmoprotectants and antioxidant compounds. In contrast, salt stress mitigation is highly species-dependent, with some microalgae enhancing stress tolerance through osmoprotectant and antioxidant accumulation, while others reduce these compounds, potentially lowering stress perception via unknown mechanisms. Despite the significance of the abiotic stress, heat stress mitigation by microalgal biostimulants remains an underexplored research area. Additionally, indirect applications of microalgae—ranging from biotechnological innovations to desalination—underscore the broader potential of these organisms in agricultural resilience. Collectively, this review identifies three key gaps in the existing literature—the diversity gap, the practical gap, and the research gap—while outlining promising avenues for future research in microalgal biostimulant development.

Ethylene is a volatile plant hormone that regulates many developmental processes and responses towards (a)biotic stress. Studies have shown that high levels of ethylene repress vegetative growth in many important crops, including tomato (Solanum lycopersicum), possibly by inhibiting photosynthesis. We investigated the temporal effects of ethylene on young tomato plants using an automated ethylene gassing system to monitor the physiological, biochemical, and molecular responses through time-course RNA-seq of a photosynthetically active source leaf. We found that ethylene evokes a dose-dependent inhibition of photosynthesis, which can be characterized by three temporally distinct phases. The earliest ethylene responses that marked the first phase and occurred a few hours after the start of the treatment were leaf epinasty and a decline in stomatal conductance, which led to lower light perception and CO₂ uptake, respectively, resulting in a rapid decline of soluble sugar levels (glucose, fructose). The second phase of the ethylene effect was marked by low carbohydrate availability, which modulated plant energy metabolism to adapt by using alternative substrates (lipids and proteins) to fuel the TCA cycle. Long-term continuous exposure to ethylene led to the third phase, characterized by starch and chlorophyll breakdown, which further inhibited photosynthesis, leading to premature leaf senescence. To reveal early (3 h) ethylene-dependent regulators of photosynthesis, we performed a ChIP-seq experiment using anti-ETHYLENE INSENSITIVE 3-like 1 (EIL1) antibodies and found several candidate transcriptional regulators. Collectively, our study revealed a temporal sequence of events that led to the inhibition of photosynthesis by ethylene and identified potential transcriptional regulators responsible for this regulation.