The Tusi manors in the Yunnan–Tibet region exemplify historical timber-framed architecture that seamlessly blends official and vernacular styles. It integrates the characteristics of ‘distinct regionality’ and ‘convergent ethnic identity’, embodies rich construction wisdom and regional ethnic culture, and serves as ‘living’ material evidence for studying regional architectural craftsmanship. Through field surveys and on-site surveying, primary data were collected to obtain architectural samples and foundational documentation of Tusi manors in the Yunnan–Tibet region. By authenticating their prototypes and integrating regional construction practices, this study analyzed the architectural characteristics of these manors, including settlement mechanisms, site selection principles, courtyard layouts, architectural typologies, spatial configurations, and functional systems from an architectural perspective. Building on this foundation, the study incorporates natural environment and socio-cultural contexts to conduct a multidimensional analysis of these characteristics. It aims to improve awareness of the cultural heritage of regional timber-framed buildings and provide a reference basis for the conservation and inheritance of these historical buildings.