Rhizosphere hypoxia, caused by soil compaction and waterlogging, is a major constraint on agricultural productivity. It severely impairs crop growth and yield by inhibiting root aerobic respiration, disrupting energy metabolism, and altering the rhizosphere microecology. Micro-nano bubbles (MNBs) show significant potential for alleviating rhizosphere hypoxia due to their unique physicochemical properties, including large specific surface area, high oxygen dissolution efficiency, prolonged retention time, and negative surface charge. This paper systematically reviews the key characteristics of MNBs, particularly their enhanced mass transfer capacity and system stability, and outlines mainstream preparation methods such as cavitation, electrolysis, and membrane dispersion. And the multiple alleviation mechanisms of MNBs—including continuous oxygen release, improvement of soil pore structure, and regulation of rhizosphere microbial communities—are clarified. The combination of MNBs aeration and subsurface drip irrigation can increase soil aeration by 5%. When applied in soilless cultivation and conventional irrigation systems, MNBs enhance crop yield and nutrient use efficiency. For example, tomato yield can be increased by 12–44%. Furthermore, the integration of MNBs with water–fertilizer integration technology enables the synchronized supply of oxygen and nutrients, thereby optimizing the rhizosphere environment efficiently. This paper sorts out the empirical effects of MNBs in soilless cultivation and conventional irrigation, and provides directions for solving problems such as “insufficient oxygen supply to deep roots” and “reactive oxygen species (ROS) stress in sensitive crops”. Despite these significant advantages, the industrialization of MNBs still needs to overcome challenges including high equipment costs and insufficient precision in parameter control, so as to promote large-scale agricultural application and provide an innovative strategy for the management of rhizosphere hypoxia.

Radiation-based treatments have emerged as important environmental and postharvest regulatory tools for improving the quality of edible mushrooms. Visible light, ultraviolet (UV) radiation, gamma irradiation, and pulsed-light treatments influence mushroom growth, morphogenesis, nutrient accumulation, antioxidant capacity, and storage performance through distinct physiological and molecular mechanisms. However, current findings remain fragmented, and a comprehensive synthesis of their regulatory effects and underlying mechanisms is lacking. This systematic review was conducted following the PRISMA 2020 framework. A structured literature search was performed in the Web of Science, PubMed, and CNKI databases. After screening and eligibility assessment, 111 studies were included in the qualitative synthesis. The available evidence indicates that radiation-based treatments exert stage-dependent and species-specific effects on edible mushrooms. Visible light primarily regulates morphogenesis through photoreceptor-mediated signaling pathways, whereas UV radiation promotes vitamin D₂ biosynthesis and antioxidant accumulation through photochemical and reactive oxygen species (ROS)-related mechanisms. Gamma irradiation and pulsed-light treatments are mainly applied during postharvest handling to suppress microbial contamination, delay browning and senescence, and extend shelf life. Based on the available evidence, a unified mechanistic framework linking signal perception, ROS regulation, transcriptional reprogramming, metabolic responses, and quality formation is proposed. Despite these advances, substantial challenges remain, including limited mechanistic understanding, insufficient integration of multi-omics evidence, lack of standardized treatment protocols, and difficulties in industrial-scale implementation. Future research should focus on multi-radiation synergistic strategies, precision environmental regulation, and intelligent cultivation systems. Overall, this review provides a comprehensive synthesis of current evidence regarding radiation-mediated quality regulation in edible mushrooms and offers a theoretical basis for optimizing mushroom production and developing sustainable postharvest preservation technologies.

Osmotic stress severely limits the growth and development of tomato (Solanum lycopersicum L.) by reducing cellular water potential, disrupting redox homeostasis, and impairing physiological functions. Micro–nano bubble (MNB) treatment can increase dissolved oxygen in the root-zone solution and improve the root-zone environment, which may benefit root metabolic activity and stress adaptation. However, the underlying molecular mechanisms are still not elucidated. To explore the underlying molecular mechanisms of how MNB-mediated root oxygenation alleviates osmotic stress in tomato, we have integrated the physiological and biochemical alterations, variable-pressure scanning electron microscopy (VP-SEM), and transcriptomic analysis (RNA-seq) under osmotic stress. The results revealed that MNBs significantly reduced PEG-induced wilting and decreased reactive oxygen species (ROS) accumulation and relative electrical conductivity (REC). Indeed, MNBs also markedly upregulated the expression of root aquaporins PIP2.7 and PIP2.4, suppressed the expression of NCED1 in leaves, and increased levels of endogenous growth-promoting hormones, including IAA and GA₃, under osmotic stress. VP-SEM observations showed that MNB-treated plants exhibited a relatively more open stomatal appearance compared with PEG-treated plants. Together, these findings suggest that MNBs mitigate PEG-induced osmotic stress in tomato, potentially by improving the root-zone aeration environment and coordinating water transport-related gene expression, antioxidant defense, and hormonal balance. These results provide a promising physical approach and theoretical basis for improving tomato stress tolerance under osmotic stress.

Plants are increasingly exposed to recurrent, combined, and fluctuating environmental stresses, making it essential to understand how transient stress signals are converted into durable adaptive states. Stress memory and cross-tolerance represent two interconnected strategies that enable plants to respond more rapidly or effectively to subsequent stresses, yet the regulatory mechanisms linking short-term responses with long-term adaptive potential remain incompletely understood. Acetylation has emerged as a dynamic regulatory interface in this process owing to its reversibility, rapid responsiveness, broad substrate range, and close coupling with cellular metabolic status. In this review, we summarize recent progress in acetylation-mediated plant stress adaptation, focusing on transcriptional bookmarking at the chromatin level, non-histone acetylation of signaling and metabolic proteins, acetyl-CoA-dependent and NAD ⁺ -dependent metabolic coupling, and regulation in different subcellular compartments, including chloroplasts, mitochondria, and the cytoplasm. We further discuss the conservation, divergence, and evidence hierarchy of key HAT/HDAC regulatory nodes in model plants and crops, highlighting that their functional outputs depend on target identity, stress context, and crop background rather than on their enzymatic identity alone. Finally, we evaluate the potential and limitations of acetylation-based crop strategies for improving crop stress resilience, including priority target selection, small-molecule regulation, epigenome editing, synthetic regulatory modules, and molecular design breeding. Overall, acetylation should not be viewed simply as a transcriptional “on/off” switch, but as a multilayered regulatory hub linking environmental signals, metabolic states, chromatin plasticity, and adaptive phenotypes. Future advances will depend on causal validation of non-histone substrates, time-resolved acetylation maps, multi-stress network dissection, and validation in crop systems under complex field conditions.

Heat stress (HS), increasingly intensified by climate change, severely restricts tomato growth and productivity. Although core heat shock factor-mediated transcriptional networks have been extensively characterized, how lipid metabolic reprogramming is transcriptionally coordinated during thermotolerance remains unclear. Using SlHDZ19 overexpression and mutant lines together with transcriptomic, biochemical, promoter-binding, and gene-silencing analyses, we show that the homeobox-leucine zipper transcription factor SlHDZ19 promotes tomato (Solanum lycopersicum) thermotolerance by activating a PLA2-dependent lipid-metabolic transcriptional program in leaves. SlHDZ19 overexpression generally improved heat-stress performance, while SlHDZ19 mutant lines exhibited heightened sensitivity, including more severe wilting, higher electrolyte leakage, and reduced proline accumulation and CAT activity under heat stress. Transcriptomic analysis revealed that SlHDZ19 is required for the full induction of canonical heat-responsive genes and that the linoleic acid metabolism pathway was repeatedly implicated in SlHDZ19-dependent transcriptional changes. SlHDZ19 binds to and activates the promoters of SlPLA_2α, which encodes a phospholipase A2 involved in releasing linoleic acid from membrane lipids, and three lipoxygenase genes (SlLox7, SlLox8, and SlLoxC), accompanied by elevated overall PLA2 and LOX activities in SlHDZ19-overexpressing plants. Moreover, genetic silencing of SlPLA_2α in both wild-type and SlHDZ19-overexpressing backgrounds supported its functional requirement downstream of SlHDZ19 in thermotolerance. Collectively, our findings support a thermotolerance module in which SlHDZ19 transcriptionally regulates PLA2- and LOX-associated steps of linoleic acid metabolism, potentially linking lipid-associated signaling and membrane remodeling with heat stress adaptation in tomato.