Immune activation, which is accompanied by the production of proinflammatory cytokines, is a strong predictor of disease progression in HIV infection. Inflammation is critical in neuronal damage linked to HIV-associated neurocognitive disorders. We examined the relationship between plasma cytokine levels and deficits in neurocognitive function. Multiplex profiling by Luminex^® technology was used to quantify 27 cytokines/chemokines from 139 plasma samples of people living with HIV (PLWH). The relationship of plasma cytokine markers, clinical parameters, and cognitive impairment, was assessed using Spearman correlations. Partial least squares regression and variable importance in projection scores were used for further evaluation of the association. Forty-nine (35.3%) participants exhibited neurocognitive impairment based on a global deficit score (GDS) of at least 0.5 and 90 (64.7%) were classified as nonimpaired. Twenty-three (16.5%) initiated on combination antiretroviral therapy for 4 weeks before cognitive assessment and 116 (83.5%) were not on treatment. We identified five proinflammatory cytokines that were significant predictors of GDS namely, IP-10 (β = 0.058; p = .007), RANTES (β = 0.049; p = .005), IL-2 (β = 0.047, p = .006), Eotaxin (β = 0.042, p = .003), and IL-7 (β = 0.039, p = .003). IP-10 and RANTES were the strongest predictors of GDS. Both cytokines correlated with plasma viral load and lymphocyte proviral load and were inversely correlated with CD4⁺ T cell counts. IP-10 and RANTES formed a separate cluster with highest proximity. Study findings describe novel associations among IP-10, RANTES, cognitive status, plasma viral load, and cell-associated viral load.

There is growing evidence of urban poverty in and around cities in sub-Saharan Africa in the form of food and nutrition insecurity. Although many studies have been done across sub-Saharan Africa on urban agriculture, food and nutrition insecurity, little is known about the association of urban agriculture to household food and nutrition insecurity along the urban–rural continuum, especially in West African cities. Therefore, a survey was carried out between August and September 2014 in and around Ouagadougou (Burkina Faso), using a transect approach to guide the data collection. The purpose of this study was to understand the dynamics of urban, periurban and rural agriculture and its association with household food and nutrition insecurity. A total of 240 households participated in the survey. From these households, data were collected on 179 women of reproductive age (15–49 yr) and 133 children under the age of 5 yr to compute Women's Dietary Diversity Scores (WDDS) and other anthropometric indices. The results of this study provide a general picture of crop production which is inclined to subsistence and income generation. Households in rural and periurban areas were more engaged in crop and livestock production compared with their urban area counterparts. Households in periurban areas had the highest relative proportion (54%) of food insecurity (household food insecurity access scale (HFIAS) > 11), compared with urban areas (39%) and rural areas (45%). At the same time the periurban households had the highest relative proportion of stunting and wasting prevalence compared with urban and rural households. Households in the rural areas had the highest dietary diversity (WDDS ≥ 6), compared with periurban and urban households. Households keeping livestock significantly experienced less wasting (weight-for-height index (WHZ)) (coef = −0.15;P= 0.008) by a factor of 0.15, and overweight (body mass index (BMI)-for-age (BAZ)) (coef = −0.12;P= 0.015) by a factor of 0.12 compared with households not doing livestock keeping. Households involved in crop production significantly experienced more food insecurity, HFIAS (coef. = 2.55;P= 0.042) by a factor of 2.55 compared with households without crop production. The complexity of periurban areas coupled with the scourge of food and nutrition insecurity will require more periurban agriculture and food policy consideration.

We aimed to perform a risk assessment in a rural setting, where drinking water is obtained from both protected and unprotected deep or shallow wells, boreholes and springs. Water is consumed untreated and this poses a risk of acquiring waterborne infections that may cause diarrhea. The study included 113 study participants who volunteered in Chiweshe rural community (Musarara village) in Mashonaland Central Province in Zimbabwe. There were 34 (30%) males and 79 (70%) females with ages ranging from 2 to 89 years. HIV counseling was carried out at the communal meeting and testing was done at home visits. Stool and drinking water samples were collected from 104 subjects. Routine laboratory methods were used to examine for parasitic infections. Only 29 (25.7%) of participants were confirmed HIV positive using 2 rapid serology tests; eighty-four (74.3%) were negative. Diarrheic stool samples were observed in 17 (16.3%) participants and of these 5 (29.4%) were HIV seropositive. Several parasites were isolated from stool samples: G. duodenalis 6 (5.7%), E. histolytica/dispar 19 (18.2%), C. parvum, 8 (7.6%) and C. cayetanensis 23 (22.1%). Eleven out of 30 (36.6%) water bodies had protozoan parasites: G. duodenalis 2 (6.6%), E. histolytica 4 (13.3%), C. parvum 1 (3.3%), C. cayetanensis 3 (10%), E. coli 1 (3.3%). The water sources were being used without treatment and were shown to pose a risk for acquiring diarrheagenic protozoan parasites.