Using morphological and cultural characteristics for identification, 36 Fusarium isolates were recovered from diseased roots, stems, and seeds of soybean from several localities throughout Vojvodina Province, Serbia. Based on molecular characterization, 12 Fusarium species were identified: F. acuminatum, F. avenaceum, F. commune, F. equiseti, F. graminearum, F. incarnatum, F. oxysporum, F. proliferatum, F. solani, F. sporotrichioides, F. subglutinans, and F. tricinctum. The elongation factor 1-α–based phylogeny grouped the isolates into 12 well-supported clades, but polymorphisms among sequences in some clades suggested the use of the species complex concept: (i) F. incarnatum-equiseti species complex (FIESC)—F. incarnatum and F. equiseti; (ii) F. oxysporum species complex (FOSC)—F. oxysporum; (iii) F. solani species complex (FSSC)—F. solani; and (iv) F. acuminatum/F. avenaceum/F. tricinctum species complex (FAATSC)—F. acuminatum, F. avenaceum, and F. tricinctum. Pathogenicity tests showed that the most aggressive species causing soybean seed rot were F. sporotrichioides, F. graminearum, FIESC, and F. avenaceum. Furthermore, F. subglutinans, FSSC, and F. proliferatum showed a high percentage of pathogenicity on soybean seeds (80 to 100%), whereas variability in pathogenicity occurred within isolates of F. tricinctum. FOSC, F. commune, and F. acuminatum had the lowest pathogenicity. To our knowledge, this is the first study of the characterization of Fusarium species on soybean in Serbia. This study provides valuable information about the composition of Fusarium species and pathogenicity that will be used in further research on soybean resistance to Fusarium-based diseases.

Weed control in corn is a major challenge due to increasing problems with highly dominant weed species and herbicide resistance evolution. Common lambsquarters and johnsongrass constitute up to 80-90% of the weed population in many spring crops such as soybeans, sunflower or corn in Serbia. Currently, acetolactate synthase (ALS)-inhibiting herbicides such as the systemic selective sulfonylurea nicosulfuron are most commonly used for chemical weed control of those species. A better understanding of the impact of nozzle type and adjuvants use on nicosulfuron efficacy can help to improve control of common lambsquarters and johnsongrass and minimize herbicide resistance development. Field trials were conducted in Serbia from 2020-2022 to evaluate the impact of two adjuvants (a non-ionic surfactant and a mineral fertilizer ammonium-sulphate) and two nozzle types (drift-reducing nozzles and flat-fan nozzles) on common lambsquarters and johnsongrass control using nicosulfuron. Satisfactory biomass reduction of common lambsquarters (83 to 87%) and johnsongrass (83 to 97%) was achieved after nicosulfuron application. Adding a non-ionic surfactant increased the biomass reduction for common lambsquarters (94 to 98%) and johnsongrass (90 to 100%) independently of the nozzle type used. Selection of nozzle type did not show consistent effects on common lambsquarters and johnsongrass control. Nicosulfuron efficacy was increased with NIS adjuvant for both nozzle types compared to nicosulfuron solo for both species, and XR nozzles on average resulted in a higher efficacy for common lambsquarters compared to TTI. Adding the mineral fertilizer ammonium-sulphate adjuvant resulted in lower biomass reduction for both nozzle types and weed species (65-78% and 61-91% for common lambsquarters and johnsongrass, respectively). Corn grain yield was predominantly influenced by annual meteorological conditions and adjuvant type added to nicosulfuron. This research suggests that addition of the non-ionic adjuvant is an essential factor for successful control of common lambsquarters and johnsongrass in corn and enables use of drift-reducing nozzles.

Geospatial sensing and data-driven technologies (GSDDTs) are playing an increasingly important role in transforming (agro)forestry practices across the Western Balkans 6 region (WB6). This review critically examines the current state of GSDDT application in six WB countries (also known as the WB6 group)—Albania, Bosnia and Herzegovina, Kosovo*, Montenegro, North Macedonia, and Serbia—with a focus on their contributions to sustainable (agro)forest management. The analysis explores the use of unmanned aerial vehicles (UAVs), light detection and ranging (LiDAR), geographic information systems (GIS), and satellite imagery in (agro)forest monitoring, biodiversity assessment, landscape restoration, and the promotion of circular economy models. Drawing on 25 identified case studies across WB6—for example, ALFIS, Forest Beyond Borders, ForestConnect, Kuklica Geosite Survey, CREDIT Vibes, and Project O₂ (including drone-assisted reforestation in Kosovo*)—this review highlights both technological advancements and systemic limitations. Key barriers to effective GSDDT deployment across WB6 in the (agro)forestry sector and its cross-border cooperation initiatives include fragmented legal frameworks, limited technical expertise, weak institutional coordination, and reliance on short-term donor funding. In addition to mapping current practices, this paper offers a comparative overview of UAV regulations across the WB6 region and identifies six major challenges influencing the adoption and scaling of GSDDTs. To address these, it proposes targeted policy interventions, such as establishing national LiDAR inventories, harmonizing UAV legislation, developing national GSDDT strategies, and creating dedicated GSDDT units within forestry agencies. This review also underscores how GSDDTs contribute to compliance with seven European Union (EU) acquis chapters, how they support eight Sustainable Development Goals (SDGs) and their sixteen targets, and how they advance several EU Green Agenda objectives. Strengthening institutional capacities, promoting legal alignment, and enabling cross-border data interoperability are essential for integrating GSDDTs into national (agro)forest policies and research agendas. This review underscores GSDDTs’ untapped potential in forest genetic monitoring and landscape restoration, advocating for their institutional integration as catalysts for evidence-based policy and ecological resilience in WB6 (agro)forestry systems.

Organic agriculture offers many benefits through the increased nutritional quality of produced crops, agro-ecosystem preservation, and climate change mitigation. The development of an efficient nutrient management strategy in low-input systems, such as organic agriculture, which supports soil fertility and essential nutrients absorption by crops, is continually exploring. Thus, a study with maize–spelt–soybean rotation during a 5-year period in organic production was established to evaluate the variability in soil organic matter (SOM) and the status of available elements: N, P, K, Ca, Mg, Fe, Cu, Mn, Zn, and Si from the soil, as well as grain yield (GY) and the content of protein, P, K, Ca, Mg, Fe, Cu, Mn, Zn, and Si concentration in the grain of spelt, maize, and soybean. Significant variations in mineral elements in the soil, GY, and grain composition were detected. Spelt achieved the highest average GY, while soybean grain was the richest in a majority of examined nutrients. The soil Ca content was important for GY, while the protein level in grain was generally tied to the Mn level in the soil. It was recognized that soil–crop crosstalk is an important strategy for macro- and micro-nutrients management in the soil and grain of organically produced spelt, maize, and soybean. While a reduction in the GY and protein concentration in grain was present over time, it was established that a low-input system under dry-farming conditions supports nutrient availability and accumulation in grain, under semi-arid agro-ecological conditions of central Serbia.

This study aimed to investigate the Diaporthe species associated with Phomopsis stem canker of sunflower (Helianthus annuus L.) in Serbia. The significant increase in sunflower and soybean (Glycine max [L.] Merr.) cultivation may have created the bridge favorable conditions for the distribution of Diaporthe species in this region. The present study identified five Diaporthe species on sunflower: D. gulyae, D. helianthi, D. pseudolongicolla, D. stewartii, and the newly identified D. riccionae based on morphological, molecular, and pathogenic characteristics. The research emphasizes the importance of effective inoculation methods and evaluates the aggressiveness of isolates. Sunflower plants were inoculated using the stem wound method, while seeds of sunflower and soybean were inoculated using the standard seed method. Most of the tested isolates demonstrated high aggressiveness, resulting in more than 80% premature wilting of sunflower plants. Additionally, this research examined the aggressiveness of Diaporthe species on sunflower seeds, highlighting D. stewartii and D. pseudolongicolla as common pathogens of both sunflower and soybean. The most aggressive species on seeds was D. stewartii, causing seed decay of up to 100% in sunflower and 97% in soybean. The findings suggest the development of resilient sunflower genotypes through breeding programs and the implementation of strategies to manage cross-contamination risks between sunflower and soybean crops. Furthermore, this study provides insights into the interactions between Diaporthe species and the seeds of sunflower and soybean. Future research will enhance our understanding of the impact of Diaporthe species on sunflower and soybean.

Soils formed on limestones occupy large area in Serbia, but there is not a lot of information about their properties. This paper presents physical and chemical properties of the Brownized Calcomelanosols and Moderately-deep Calcocambisols of the Ozren Mountain. Brownized Calcomelanosols under natural grassy vegetation were investigated at the Vlasina pass, whereas Calcocambisols under mixed forests were investigated near the site "Ozrenske livade". Soil profiles were excavated up to the parent material, and disturbed and undisturbed soil samples were collected from soil genetic horizons. Following soil characteristics were determined: particle size distribution, soil structure and water resistance of structural aggregates, bulk density, total porosity, air capacity, water-holding capacity, water conductivity, pH, content of humus, available phosphorus (P2O5) and potassium (K2O), hydrolytic acidity, cation exchange capacity (CEC), sum of exchangeable base cations, and base saturation. Humus-accumulative horizon (Amo) of the Brownized Calcomelanosols has silty clay texture, whereas the cambic (B)rz horizon of both soils has a clay texture. The Amo horizon of Calcomelanosols is characterized by fine to medium granular structure (0.5-5 mm) of very high water resistance. The total porosity, air capacity and water permeability of both soils is high. Their water-holding capacity is high. Amo horizon of Calcomelanosols is characterized by a high humus content (>10%). Soil reaction in water of Amo horizon is neutral, whereas in (B)rz horizons both soils are moderately acid. Both soils have high CEC (>58 cmol kg-1 ) and base saturatation (>95%). Brownized Calcomelanosol is moderately to highly supplied with available K2O, whereas Calcocambisol is moderately supplied; while both soils are very poor in available P2O5. Since this study presents quite favorable physical and chemical properties of the investigated soils due to the conservation of natural forests and grasslands, the results can help in a deeper understanding of soil ecology and the preservation of natural plant cover.