Wood in service is sequestering carbon, but it is principally prone to deterioration where different fungi metabolize wood, and carbon dioxide is released back to the atmosphere. A key prerequisite for fungal degradation of wood is the presence of moisture. Conversely, keeping wood dry is the most effective way to protect wood from wood degradation and for long-term binding of carbon. Wood is porous and hygroscopic; it can take up water in liquid and gaseous form, and water is released from wood through evaporation following a given water vapour pressure gradient. During the last decades, the perception of wood-water relationships changed significantly and so did the view on moisture-affected properties of wood. Among the latter is its susceptibility to fungal decay. This paper reviews findings related to wood-water relationships and their role for fungal wood decomposition. These are complex interrelationships not yet fully understood, and current knowledge gaps are therefore identified. Studies with chemically and thermally modified wood are included as examples of fungal wood substrates with altered moisture properties. Quantification and localization of capillary and cell wall water – especially in the over-hygroscopic range – is considered crucial for determining minimum moisture thresholds (MMThr) of wood-decay fungi. The limitations of the various methods and experimental set-ups to investigate wood-water relationships and their role for fungal decay are manifold. Hence, combining techniques from wood science, mycology, biotechnology and advanced analytics is expected to provide new insights and eventually a breakthrough in understanding the intricate balance between fungal decay and wood-water relations.Key points: • Susceptibility to wood-decay fungi is closely linked to their physiological needs.• Content, state and distribution of moisture in wood are keys for fungal activity.• Quantification and localization of capillary and cell wall water in wood is needed.• New methodological approaches are expected to provide new insights

There is increasing awareness of the durability, dimensional stability, and strength properties of thermally modified timber products. Appropriate standards and test methods for industrial-scale quality control are needed. The aim of this study was to investigate the suitability of CIE L*a*b* color measurements for implementation in industrial quality control. Good linear correlation was found between a cumulated measure of L* and b* data and the heat treatment intensity for spruce (Picea abies Karst.), pine (Pinus sylvestris L.), and beech (Fagus sylvatica L.). Wood samples homogenized by milling revealed less variation in color values compared to wood surface measurements. Thus, measurements on milled wood are recommendable to obtain results with high statistical significance. Color measurements are rapid, precise, and highly reproducible. Based on the strong correlation between color data and heat-treatment intensities, color measurements could easily be implemented for quality control of thermally modified timber.

Chemically or physically modified wood materials have enhanced resistance to wood decay fungi. In contrast to treatments with traditional wood preservatives, where the resistance is caused mainly by the toxicity of the chemicals added, little is known about the mode of action of nontoxic wood modification methods. This study reviews established theories related to resistance in acetylated, furfurylated, dimethylol dihydroxyethyleneurea-treated, and thermally modified wood. The main conclusion is that only one theory provides a consistent explanation for the initial inhibition of brown rot degradation in modified wood, that is, moisture exclusion via the reduction of cell wall voids. Other proposed mechanisms, such as enzyme nonrecognition, micropore blocking, and reducing the number of free hydroxyl groups, may reduce the degradation rate when cell wall water uptake is no longer impeded.

The need for a reliable database and a corresponding methodology to predict service life of wood and wood-based products emanates from the European Construction Products Directive, as well as from a strong interest by the building industry. A literature review on decay-influencing factors and on previous approaches to implement methods and tools for service life estimations was the basis for a concept for service life prediction of wooden components. The effect of various indirect decay factors was found to be reducible to a few direct decay factors, with material resistance, as well as wood moisture content, wood temperature and their dynamics among the key factors for fungal decay. Approaches that consider only indirect factors, e.g. climate indices, suffer from poor correlation with service life or decay rates. Therefore, the overall consideration of all possible influences, reduced on the key direct decay factors in first instance, is recommended. To fill a theoretical model with a reliable database, various data sources need to be considered, in decreasing order of priority: in-service performance, field test data, laboratory test data, survey analysis and expert opinion.