A study was conducted to determine the total microbial population, the occurrence of growth promoting bacteria and their beneficial traits in acid sulfate soils. The mechanisms by which the bacteria enhance rice seedlings grown under high Al and low pH stress were investigated. Soils and rice root samples were randomly collected from four sites in the study area (Kelantan, Malaysia). The topsoil pH and exchangeable Al ranged from 3.3 to 4.7 and 1.24 to 4.25 cmol_c kg⁻¹, respectively, which are considered unsuitable for rice production. Total bacterial and actinomycetes population in the acidic soils were found to be higher than fungal populations. A total of 21 phosphate-solubilizing bacteria (PSB) including 19 N₂-fixing strains were isolated from the acid sulfate soil. Using 16S rRNA gene sequence analysis, three potential PSB strains based on their beneficial characteristics were identified (Burkholderia thailandensis, Sphingomonas pituitosa and Burkholderia seminalis). The isolated strains were capable of producing indoleacetic acid (IAA) and organic acids that were able to reduce Al availability via a chelation process. These PSB isolates solubilized P (43.65%) existing in the growth media within 72 hours of incubation. Seedling of rice variety, MR 219, grown at pH 4, and with different concentrations of Al (0, 50 and 100 µM) was inoculated with these PSB strains. Results showed that the bacteria increased the pH with a concomitant reduction in Al concentration, which translated into better rice growth. The improved root volume and seedling dry weight of the inoculated plants indicated the potential of these isolates to be used in a bio-fertilizer formulation for rice cultivation on acid sulfate soils.

Aluminum toxicity is widely considered as the most important limiting factor for plants growing in acid sulfate soils. A study was conducted in laboratory and in field to ameliorate Al toxicity using plant growth promoting bacteria (PGPB), ground magnesium limestone (GML) and ground basalt. Five-day-old rice seedlings were inoculated by Bacillus sp., Stenotrophomonas maltophila, Burkholderia thailandensis and Burkholderia seminalis and grown for 21 days in Hoagland solution (pH 4.0) at various Al concentrations (0, 50 and 100 μM). Toxicity symptoms in root and leaf were studied using scanning electron microscope. In the field, biofertilizer (PGPB), GML and basalt were applied (4 t·ha⁻¹ each). Results showed that Al severely affected the growth of rice. At high concentrations, the root surface was ruptured, leading to cell collapse; however, no damages were observed in the PGPB inoculated seedlings. After 21 days of inoculation, solution pH increased to >6.0, while the control treatment remained same. Field study showed that the highest rice growth and yield were obtained in the bio-fertilizer and GML treatments. This study showed that Al toxicity was reduced by PGPB via production of organic acids that were able to chelate the Al and the production of polysaccharides that increased solution pH. The release of phytohormones further enhanced rice growth that resulted in yield increase.

Acid sulfate soils cover an estimated 13 million hectares (ha) worldwide, the largest concentration (6.7 million ha) being in the coastal lowlands of Southeast Asia. In Malaysia, they occur mainly ...

To mitigate global climate change and simultaneously increase soil productivity, the use of biochar in agriculture can be a modern agro-technology that can help in reducing greenhouse gas emissions, enhancing soil carbon sequestration, and ultimately increasing crop yield. This study aimed to evaluate the effects of biochar and lime application on the chemical properties of acid soil and the emission of CO₂. A 60-day incubation study was conducted with eleven treatments (T) in which two different biochar produced from rice husk (RHB) and oil palm empty fruit bunches (EFBB) at two rates (10 and 15 t ha⁻¹) and on three rates of dolomitic limestone (100%, 75%, and 50%), recommended rate of NPK and a control (no amendment). The result showed that biochar and lime significantly increased soil pH, available P, and decreased exchangeable Al compared to the control. The pH increase was 44.02% compared to the control treatment on day 15, and the available P was found to be 22.44 mg kg⁻¹ on day 30 from Treatment 7 (75% lime + 15 t ha⁻¹ RHB). The cumulative CO₂ emission from T₇ was 207.40 μmol CO₂ m⁻² that decreased 139.41% compared to the control. Our findings conclude that RHB with 75% lime has more potential than EFBB to increase nutrient availability and reduce the emission of CO₂ in acid soil.