Agricultural diversification represents an important strategy for promoting sustainability and resilience in rural regions. Goji berries (Lycium barbarum) have emerged as a promising alternative crop due to their high nutritional and functional potential. In this sense, the search for new crops to diversify the production in southwestern Spain is of main interest for farmers to adapt their productions to consumers claims and to climate change, having alternatives to the classical crops (olives, grapes for wine, etc.). This study evaluated several quality related parameters of three goji berry varieties cultivated in the southwest of Spain. Texture profile analysis (TPA) and puncture tests revealed varietal differences in firmness, cohesiveness, and springiness, influenced by genotype and harvesting time. Other morphological and quality parameters such as moisture, total soluble solids, titratable acidity and color were also affected. Significant differences in antioxidant capacity (ABTS and DPPH assays) were found among the varieties and harvesting times, with NQ7 exhibiting the highest values. Phenolic compounds were identified and quantified by LC–HRMS/MS, detecting 33 compounds, with most belonging to the hydroxycinnamic acids, flavonols and flavanones families. NQ7 presented the highest total phenolic content (74.787 mg/100 g DW), with rutin, coumaric acid derivatives, and naringenin as major contributors. The correlation analysis confirmed a strong relationship between total phenolic content and antioxidant capacity. Overall, the results indicated that goji berries grown in southwestern Spain exhibited favorable quality and bioactive profiles, supporting their suitability for sustainable production and commercialization, including further applications as functional food ingredients.

In a context of increasing water scarcity in southern Spain, improving water-use efficiency has become essential for sustainable crop production. This study evaluated the effects of two irrigation regimes (100% and 75% of crop evapotranspiration, ETc) on the quality attributes and phenolic compound profile of three goji berry varieties (NQ1, Sweet Lifeberry, and Turgidus) cultivated under organic conditions in southwestern Spain. Key quality parameters including color, sugars, acidity, firmness, moisture, size, and weight were evaluated together with phenolic compounds profiling using UHPLC-MS/MS and antioxidant activity assays. The goji berry varieties studied showed significant differences (p < 0.05) in most of the variables analyzed, confirming the dominant influence of genotype. In contrast, no significant differences were detected in most quality attributes between berries cultivated under full irrigation (100% ETc) and deficit irrigation (75% ETc). However, deficit irrigation promoted the accumulation of hydroxycinnamic and hydroxybenzoic acids, while flavonols and flavan-3-ols showed a decreasing tendency. Despite these compositional shifts, antioxidant capacity was generally higher under full irrigation. Among the varieties, NQ1 berries exhibited the highest total phenolic content and antioxidant activity. Harvesting time further influenced phenolic distribution and antioxidant behavior. These findings suggest that moderate deficit irrigation can be applied as a sustainable water-saving strategy for goji berry cultivation under Mediterranean conditions without compromising fruit quality.