This study aimed to compare the acute effects of three eccentric training strategies—constant resistance (CR), accentuated eccentric loading (AEL), and accelerated eccentrics (AE)—on the performance and biomechanical characteristics of the concentric phase of the squat, while maintaining a consistent squat depth. Twenty-four experienced resistance-trained male collegiate athletes (age: 21.92 ± 2.66 years; height: 175.88 ± 4.39 cm; body mass: 73.18 ± 8.08 kg) were recruited. A randomized crossover design was employed, where participants completed three squat protocols (eccentric load/concentric load/eccentric duration): AEL (90% 1RM/60% 1RM/2 s), CR (60% 1RM/60% 1RM/2 s), and AE (60% 1RM/60% 1RM/as fast as possible). Throughout the squats, kinematic and kinetic data were synchronously collected using an 8-camera 3D infrared motion capture system and two 3D force plates. The mean concentric barbell velocity in the AE condition was significantly higher than in both the AEL and CR conditions (p < 0.001). Furthermore, the AE condition demonstrated significant advantages in multiple biomechanical variables, including peak ground reaction force, as well as peak angular velocity and peak joint moments of the three lower limb joints (p < 0.05). With identical concentric loads and range of motion, increasing the velocity of the eccentric phase significantly enhances subsequent concentric performance and force output. In contrast, while the AEL strategy increases the mechanical load during the eccentric phase, its potentiating effect on concentric performance is relatively limited. These findings suggest that eccentric velocity may be a more critical variable than eccentric load in strength training.