The most used methods for the maturation process are vacuum (wet-aged) and dry (dry-aged), which can influence the microbiological quality and safety of meat for consumption. In this study, we aimed to verify the differences in microbiological quality between beef (Longissimus dorsi) that was wet-aged and dry-aged for 30 days, by quantification of indicator microorganism groups and molecular identification of Salmonella, Listeria monocytogenes, and diarrheagenic Escherichia coll. This study verified that the meat matured by the dry-aged method showed significantly lower counts of total coliforms, aerobic mesophiles, psychrotrophs, and molds and yeasts as compared to wet-aged meat. While the Salmonella spp. was not isolated in any beef sample, L. monocytogenes and enteropathogenic E. coil (EPEC), and shiga toxin-producing E. coli (STEC) and enterohemorrhagic E. coli (EHEC) were isolated only from wet-aged beef. Thus, it was concluded that the superficial dehydration of the meat during dry-aged maturation, if carried out correctly and hygienically, confers higher microbiological quality and can reduce the occurrence of microbiological hazards.

The study evaluated the effect of transportation over long distances on cattle muscle tissue of submitted to emergency slaughter in slaughterhouses in northern Tocantins, Brazil. The evaluations consisted in pH, muscle and liver glycogen, muscle histopathology and creatine kinase (CK), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity. Animals were placed into two groups: Experimental Group (EG), consisting of 19 bovines intended for immediate emergency slaughter, and Control Group (CG), composed of 24 bovines slaughtered in accordance with the normal flow. CK and ALT levels were high in EG. AST did not differ between groups. EG showed higher muscle pH and mean of degenerate fibers, mainly on the intercostal. However, muscle and liver glycogen did not differ between groups. In conclusion, cattle transported over long distances and subjected to immediate emergency slaughter showed markedly stress condition, with changes in biochemical parameters in the muscle tissue, determined by cellular degeneration.

The objective of this study was to evaluate the animal performance, carcass characteristics and meat quality of F1 Angus-Nellore steers and heifers, feedlot finished with high degree of subcutaneous fat deposition. The experimental design was completely randomized, with two treatments (steers and heifers) and 11 replicates (animals). Twenty-two bovines with initial age of 18 months of age, 11 steers and 11 heifers, with initial body weight of, respectively, 354.86±43,66 and 353.82±39,80 kg were used. The animals were finished in confinement (114 days) with a diet containing 200 g kg-1 of corn silage and 800 g kg-1 of concentrate composed mainly of corn grain and soybean meal. The dry matter intake (9.42 vs. 9.24 kg day-1), mean daily weight gain (1.44 vs. 1.38 kg day-1), feed conversion (6.49 vs. 7.05 kg of dry matter kg-1 of weight gain), hot carcass weight (284.62 vs. 268.16 kg) and the subcutaneous fat thickness (12.09 vs. 13.05 mm) were similar between steers and heifers (respectively). The hot carcass yield was higher (P < 0.05) for steers (58.14 vs. 55.53%). The steers presented a higher proportion of the forequarter (39.26 vs. 37.53%), while heifers had a higher proportion of pistol cut (49.87 vs. 48.85%). The meat of steers showed darker color (P < 0.05). The shear force of the meat and marbling score did not differ between sexual conditions, with mean values of 5.43 kgf cm-2 and 10.14 points (median), respectively. Except for the proportion of primary cuts, carcass yield and meat color, F1 Angus-Nellore heifers present similar animal performance, carcass and meat characteristics in relation to steers, feedlot finished with high degree of subcutaneous fat deposition.

The aim of this study was to evaluate the energy value of diets containing babassu cake as a replacement for Tifton-85 hay (70, 140, 210, 280 and 350 g kg-1 dry matter, DM), using the technique of indirect calorimetry. Twenty-five castrated male Santa Inês sheep, with an average body weight of 49.6 + 9.4 kg, were used in a completely randomised design. The trial was carried out in an individual open-flow respirometry chamber for small ruminants, with each animal remaining for 24 hours inside the chamber. Gross energy and net energy intake were not affected by adding the cake. Digestible energy intake and metabolisable energy intake showed a linear response, ranging from 145.44 to 178.40 and from 121.85 to 158.08 kcal kg-0.75 day-1 respectively. The energy lost through faeces and methane (% of gross energy intake, GEI) showed decreasing linear behaviour, while the caloric increase, in %GEI, showed a quadratic response. The values for digestible energy and metabolisability presented an increasing linear response. The partial efficiency of use of metabolisable energy for maintenance ranged from 0.71 to 0.81, and showed a quadratic response; however, the net energy values did not differ. For every 10 g of added by-product, there was a reduction of 0.31 g of methane per kg of DM intake. The addition of babassu cake to replace up to 350 g kg-1 DM in sheep diets increases the energy efficiency of the diet and reduces methane emission by the animals.