The worldwide climate changes every year due to global warming, waterlogging, drought, salinity, pests, and pathogens, impedes crop productivity. Brassica napus is one of the most important oil crops in the world, and rapeseed oil is considered one of the most health-beneficial edible vegetable oils. Recently, miRNAs have been found and confirmed to control the expression of targets under disruptive environmental conditions. The mechanism is through the formation of the silencing complex that mediates post-transcriptional gene silencing, which pairs the target mRNA and target cleavage and/or translation inhibition. However, the functional role of miRNAs and targets in B. napus is still not clarified. This review focuses on the current knowledge of miRNAs concerning development regulation and biotic and abiotic stress responses in B. napus. Moreover, more strategies for miRNA manipulation in plants are discussed, along with future perspectives, and the enormous amount of transcriptome data available provides cues for miRNA functions in B. napus. Finally, the construction of the miRNA regulatory network can lead to the significant development of climate change-tolerant B. napus through miRNA manipulation.

Remote sensing has been used as an important means of monitoring crop growth, especially for the monitoring of the formation of crop yield in the middle and late growth period. The information acquisition on the yield formation period of winter wheat is of great significance for winter wheat growth monitoring, yield estimation and scientific management. Hence, the main goal of this study was to verify the possibility of monitoring the grain-filling process of winter wheat and its in-field variability using an alternative non-destructive method based on orbital remote sensing. High-resolution satellite imageries (3 m) were obtained from the PlanetScope platform for three commercial winter wheat fields in Jiangsu Province, China during the reproductive stage of the winter wheat (185–215/193–223/194–224 days after sowing (DAS)). Based on the quantitative analysis of vegetation indices (VIs) obtained from high-resolution satellite imageries and three indicators of the winter wheat grain-filling process, linear, polynomial and logistic growth models were used to establish the relationship between VIs and the three indicators. The research showed a high Pearson correlation (p < 0.001) between winter wheat maturity and most VIs. In the overall model, the remote sensing inversion of the dry thousand-grain weight has the highest accuracy and its R² reaches more than 0.8, which is followed by fresh thousand-grain weight and water content, the accuracies of which are also considerable. The results indicated a great potential to use high-resolution satellite imageries to monitor winter wheat maturity variability in fields and subfields. In addition, the proposed method contributes to monitoring the dynamic spatio-temporality of the grain-filling progression, allowing for more accurate management strategies in regard to winter wheat.

Grain yield is one of the most critical indicators for evaluating the performance of wheat breeding. However, the assessment process, from early-stage breeding lines to officially registered varieties that have passed the DUS (Distinctness, Uniformity, and Stability) test, is often time-consuming and labor-intensive. Multispectral remote sensing based on unmanned aerial vehicles (UAVs) has demonstrated significant potential in crop phenotyping and yield estimation due to its high throughput, non-destructive nature, and ability to rapidly collect large-scale, multi-temporal data. In this study, multi-temporal UAV-based multispectral imagery, RGB images, and canopy height data were collected throughout the entire wheat growth stage (2023–2024) in Xuzhou, Jiangsu Province, China, to characterize the dynamic growth patterns of both breeding lines and registered cultivars. Vegetation indices (VIs), texture parameters (Tes), and a time-series crop height model (CHM), including the logistic-derived growth rate (GR) and the projected area (PA), were extracted to construct a comprehensive multi-source feature set. Four machine learning algorithms, namely a random forest (RF), support vector machine regression (SVR), extreme gradient boosting (XGBoost), and partial least squares regression (PLSR), were employed to model and estimate yield. The results demonstrated that spectral, texture, and canopy height features derived from multi-temporal UAV data effectively captured phenotypic differences among wheat types and contributed to yield estimation. Features obtained from later growth stages generally led to higher estimation accuracy. The integration of vegetation indices and texture features outperformed models using single-feature types. Furthermore, the integration of time-series features and feature selection further improved predictive accuracy, with XGBoost incorporating VIs, Tes, GR, and PA yielding the best performance (R² = 0.714, RMSE = 0.516 t/ha, rRMSE = 5.96%). Overall, the proposed multi-source modeling framework offers a practical and efficient solution for yield estimation in early-stage wheat breeding and can support breeders and growers by enabling earlier, more accurate selection and management decisions in real-world production environments.