Cyanoacrylate-based adhesives are widely used in wound closure, providing good cosmetic results and little discomfort. However, reports in the literature are found about negative effects that include the release of cytotoxic chemicals during biodegradation. In this study, we sought to evaluate and compare the effectiveness of four cyanoacrylate-based adhesives on the closure of skin incisions in Rattus norvegicus. The animals (n = 140) were divided into five groups of 28 animals each according to the wound closure technique: G1 and G2 (n-2-ethyl-cyanoacrylate); G3 (n-2-butyl-cyanoacrylate); G4 (n-2-octyl-cyanoacrylate); and G5 (5 nylon stitches). Midline incisions measuring 5.0 cm in length were created and closed using the different materials evaluated, and on D3, D7, D14, and D21, tensiometric and histopathological analyses were performed. Shorter wound closure and adhesion times were observed in G4 animals. At D3 and D7, G5 presented greater tensiometric resistance in the animals of G5, with a decrease in D14 and D21 compared to the other groups. On the other hand, the wounds of G3 and G4 were more resistant in D14 and D21, reaching maximum resistance values. Polymorphonuclear and mononuclear cells are more prevalent and more granulation tissue was observed in G5. The deposition of type III collagen was more evident in G5, whilst there was no difference in the amount of type I collagen in any of the groups treated with cyanoacrylate adhesives. Larger areas stained positive for VEGF-α in G2 and smaller areas in G4, with peaks at D7 and D14. In general, cyanoacrylate adhesives cause less intense inflammatory reactions, resulting in shorter healing times when compared to nylon sutures.

The objective of this study was to evaluate the effect of feed restriction on some physical and blood parameters in horses kept outdoors under natural conditions. Twenty horses were deprived of food for 48 h. They were closely monitored and examined, and blood samples were taken at the beginning (0) of the experiment and 6, 12, 18, 24, 30, 36, 42 and 48 hours afterward. During the experimental period, the control group (12 animals) had free access to water and hay, while the restricted group had free access to water only. Data were submitted to two-way analysis of variance with repeated measures, and statistical significance was P ≤ 0.05. The horses tolerated feed restriction without complications. Feed restriction had no effect on body mass and body condition score, heart rate, respiratory rate, capillary filling time and body temperature. However, feed restriction decreased the intensity of gastrointestinal sounds (P<0.05) compared to the control horses. Feed restriction did not cause any changes in erythrocyte variables and gamma glutamyl transferase, creatinine, total protein, and albumin concentrations. During fasting, there was a reduction in the leukocyte response (P<0.05). Feed restriction significantly raised the levels of blood urea nitrogen (24 to 48 hours), aspartate aminotransferase (36 to 48 hours) and total cholesterol (42 to 48 hours). During 48 hours of fasting, there was a continuous increase in triglyceride concentration. Feed restriction for 48 h had a marked effect on the intensity of gastrointestinal sounds and was responsible for important metabolic changes in the healthy horses of our sample. O objetivo deste estudo foi avaliar o efeito da restrição alimentar sobre parâmetros físicos e sanguíneos em equinos mantidos a pasto de Tifton (Cynodon spp) e em condições naturais. Vinte cavalos foram privados de alimento por 48 h. Eles foram monitorados, examinados e amostras de sangue foram coletadas no início (0) do experimento e 6, 12, 18, 24, 30, 36, 42 e 48 horas depois. Durante o período experimental, o grupo controle (12 animais) teve livre acesso a água e feno, enquanto o grupo restrito teve livre acesso apenas a água. Os dados foram submetidos à análise de variância bidirecional com medidas repetidas e a significância estatística foi P ≤ 0,05. Os cavalos toleraram a restrição alimentar sem complicações. A restrição alimentar por 48 h não teve efeito sobre a massa corporal e escore de condição corporal, frequência cardíaca, frequência respiratória, tempo de enchimento capilar e temperatura corporal. No entanto, o jejum alimentar diminuiu a intensidade dos sons gastrointestinais (P <0,05) em comparação com os cavalos do grupo controle. Não houve alteração nas variáveis eritrocitárias e nas concentrações de gama glutamil transferase, creatinina, proteína total e albumina. Durante o jejum, houve redução da resposta leucocitária (P <0,05) e, aumento significativo dos níveis de nitrogênio uréico no sangue (24 a 48 horas), aspartato aminotransferase (36 a 48 horas) e colesterol total (42 a 48 horas). Durante 48 horas de jejum alimentar, houve aumento contínuo da concentração de triglicerídeos. A restrição alimentar por 48 h teve um efeito marcante na intensidade dos sons gastrointestinais e foi responsável por importantes alterações metabólicas em cavalos saudáveis.

Excessive infection and inflammation are the most common complications associated with castration. The objective of this study was to compare the efficacy of flunixin meglumine (FM), meloxicam (MX), or firocoxib (FX) for inflammation control after castration in horses using acute-phase proteins (APP) as markers of inflammation. Thirty healthy, unbroken, mixed-breed horses (body weight 358.62±45.57kg and age 4.99±2.63 years) were randomly (n=10 animals/group) allocated to receive one of three different post-castration anti-inflammatory medicines: Group 1 (FM 1.1mg/kg bwt, IV, s.i.d for 5 days); Group 2 (MX 0.6mg/kg bwt, IV, s.i.d for 5 days); and Group 3 (FX 0.1mg/kg bwt, IV, s.i.d for 5 days). All horses were castrated in standing position, using the open technique. Serum and peritoneal APP concentrations were measured by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and determined before castration (0), and 3, 5, 24, 48, 72, 120 and 168 hours after castration. The results were submitted to analysis of variance using the SAS statistical program, and means were compared by the Student-Newman-Keuls test (p<0.05). Three animals from the MX group developed hyperthermia (with rectal temperatures of 39.8, 39.3 and 38.9°C on day 4, 5 and 6, respectively) and showed local clinical signs of inflammation (inguinal and excessive scrotal edema) and reluctance to walk, as well as a rigid gait of the hind limbs. The same complications were observed in one FX horse. No complications were observed among the FM animals. The castration resulted in significant changes in serum and peritoneal values of total proteins, ceruloplasmin (Cp), transferrin (Tf), albumin (Alb), haptoglobin (Hp) and α1-acid glycoprotein (Gp) in animals of all experimental groups. However, the animals of the MX and FX groups presented more intense acute phase response compared to the animals of the FM group. Changes in the APP were associated with the surgical trauma of castration, but the differences between groups were associated with the ability of the nonsteroidal anti-inflammatory drug to control the inflammation. In conclusion, and based on the findings of acute phase proteins, flunixin is more efficient to control the magnitude of inflammation following castration as compared to meloxicam and firocoxib.