Cassava (Manihot esculenta Crantz) is a major dual-purpose crop in tropical and subtropical regions, but its growth and yield are significantly constrained by abiotic stresses. Calmodulin-binding transcription activators (CAMTAs) are key regulators involved in plant development and stress responses. In this research, six CAMTA genes (MeCAMTAs) were identified from the first telomere-to-telomere (T2T) genome assembly of cassava, and these genes are distributed on four chromosomes. These genes are divided into three different subfamilies based on phylogenetic relationships. Homology analysis shows that there is one pair of replication gene pairs. Analysis of cis-acting elements reveals that the promoter regions of the MeCAMTA gene family contain cis-acting elements responsive to hormones, abiotic stresses, growth and development, and light. Through analysis of the expression patterns of MeCAMTAs in different tissues, MeCAMTAs are expressed in all tissues, among which MeCAMTA3 and MeCAMTA4.1 are mainly expressed in stems, while MeCAMTA1, MeCAMTA2, MeCAMTA4.2, and MeCAMTA6 are mainly expressed in roots. qRT-PCR analysis shows that MeCAMTAs exhibit dynamic expression patterns under different abiotic stress treatments. Therefore, this study provides a certain reference basis for the research on the abiotic stress response mechanism of cassava and also provides potential genetic resources for the stress-resistant breeding of cassava.