Turmeric (Curcuma longa L., Zingiberaceae) is a widely consumed spice and functional food valued for its bioactive constituents. Using an activity-guided strategy, this study identified the dichloromethane fraction as the most potent anti-inflammatory fraction, exhibiting markedly stronger inhibition of reactive oxygen species (ROS) production than ibuprofen (IC₅₀ $\le$ 0.4 vs. 11.2 μg/mL). Bioassay-guided purification yielded bisacurone (1), didemethoxycurcumin (2), and β-turmerone (3), with compounds 1 and 2 reported here for the first time in this fraction. Among them, β-turmerone displayed the strongest anti-inflammatory activity (IC₅₀ = 4.7 μg/mL), consistent with in silico docking and molecular dynamics analyses, revealing greater binding affinity and complex stability with myeloperoxidase (ΔG_bind = −20.90 vs. −18.89 kcal/mol for ibuprofen). Gas chromatography–mass spectrometry (GC-MS) profiling revealed a phytochemical profile dominated by turmerones and curlone, correlating with the observed bioactivity. None of the fractions exhibited acute toxicity in brine shrimp lethality assays, indicating a favorable preliminary safety profile. Our findings demonstrate the value of activity-guided isolation combined with computational validation for identifying turmeric-derived bioactives with promising nutraceutical potential, warranting further in vivo evaluation.

This study investigates the proximate composition, mineral content, and fatty acid profile of Canavalia ensiformis L. beans extracted with various solvents. Proximate analysis revealed a high protein content (23.16%), moderate fiber levels (7.13%), and a total fat content of 2.35%. Elemental analysis identified potassium (18,696.87 mg/kg) as the most abundant macroelement, followed by calcium (1,886.81 mg/kg) and magnesium (1,500.98 mg/kg), whereas iron (50.43 mg/kg) and zinc (33.28 mg/kg) were the predominant microelements. Gas chromatography analysis of fatty acids showed that monounsaturated fatty acids (MUFA) dominated the lipid profile, particularly in the hexane (58.67%), ethanol (59.57%), and raw bean (61.11%) extracts. Oleic acid (C18:1n9c) was the most abundant MUFA, reaching 51.39% in the hexane extract. Saturated fatty acids (SFA) were highest in the hexane extract (21.35%), primarily due to palmitic acid (C16:0, 18.77%). Polyunsaturated fatty acids (PUFA) were most prevalent in the hexane extract (16.23%) and bean sample (16.08%), with linoleic acid (C18:2n6c) being the major component. The aqueous extract contained notable amounts of γ-linolenic acid (C18:3n6, 1.68%). These findings highlight the nutritional potential of C. ensiformis beans and suggest that solvent selection significantly influences lipid composition. The high MUFA and PUFA content, particularly in hexane and ethanol extracts, underscores their possible applications in food and pharmaceutical formulations. Key words: Canavalia ensiformis L., GC analysis, proximate analysis, micro- and macroelements, fatty acids.

Nutrient profiling models such as the Health Star Rating (HSR) are widely used to guide consumer food choices. However, nutrient-based models may not fully

The article presents a comparative assessment of the nutritional value of beef, mutton, and horse meat sold in the markets of two regions of Kazakhstan: the Western region (Atyrau Region) and Eastern Kazakhstan (the Rudny Altai area). Significant interregional differences were identified: meat from Western Kazakhstan is characterized by higher levels of protein, calcium, phosphorus, and iron, whereas meat from the Eastern region has higher levels of intramuscular fat, zinc, manganese, and vitamin E. The meat with the highest protein content is beef from Western Kazakhstan (24.21±0.21%). Lamb from Eastern Kazakhstan contains the highest amount of fat (4.91±0.09%). Among the meat types studied, beef demonstrated the highest energy value (132.1−133.4 kcal/100 g). In samples from the Western region, calcium content reaches 22.4±0.7 mg/100 g (horse meat), phosphorus 130.6±3.9 mg/100 g (beef), and iron 2.55±0.08 mg/100 g (beef). In the Eastern region, higher concentrations of zinc (up to 3.13±0.09 mg/100 g in horse meat) and manganese (up to 0.110±0.003 mg/100 g in beef and horse meat) were recorded. An elevated content of α-tocopherol was also observed in horse meat from Eastern Kazakhstan (0.765±0.023 mg/100 g), whereas lamb from Western Kazakhstan is characterized by high levels of vitamin B 3 (11.040±0.331   mg/100   g).   The   data   obtained   indicate   a significant influence of regional natural and geochemical conditions on the formation of the nutritional profile of meat products and support the inclusion of beef, lamb, and horse meat in the daily diet to meet the requirements for protein, macroand micronutrients, and vitamins. The results can be used in the development of region-specific recommendations for rational and functional nutrition, as well as in food quality and safety control systems.