Nitrous oxide is a potent greenhouse gas, with N fertilizers being one of its major sources. Plant growth-promoting bacteria have been used to mitigate N₂O emissions by improving N use efficiency in plants. In addition, some of these microorganisms are capable of reducing N₂O to N₂, a process that could be further explored as a complementary mitigation strategy. This study aimed to test whether Azospirillum brasilense strains Ab-V5 and Ab-V6, and strain Wa3, as well as Nitrospirillum viridazoti strain BR 11145, already used in commercial inoculants for N-fertilized crops and known to carry the nosZ gene encoding nitrous oxide reductase, could act as biological sinks for this gas. In the pot experiment, soils fertilized with N and inoculated with N. viridazoti exhibited consistently lower N₂O emissions, but not when A. brasilense strains were used. The mitigation effect was observed both in bare soil (72% emission reduction) and in the presence of millet plants (60% emission reduction), confirming the ability of N. viridazoti strain BR 11145 to consume N₂O. In a field experiment conducted with maize, inoculation with N. viridazoti again reduced N₂O fluxes during the first two weeks after fertilization compared with the urea-only treatment. However, no significant differences were detected comparing emission factors, whose calculation requires consideration of the entire monitoring period, thereby adding more variability. While N₂O mitigation was observed, no significant effects of inoculation or N fertilization were found on maize growth or yield. Nonetheless, the consistent reduction in N₂O emissions achieved with N. viridazoti strain BR 11145 suggests that inoculants with this bacterium represent a promising biological sink for N₂O, offering a novel nature-based solution to enhance the sustainability of tropical crop management.