This study focuses on Populus ×euramericana (Dode) Guinier, a globally distributed fast-growing tree. Despite its valuable wood, it exhibits low durability. The aim of this study was to assess the efficacy of a binary composite comprising silver nanoparticles (AgNPs) and chitosan oligomers (COS) in protecting P. ×euramericana ‘I-214’ wood against degradation caused by xylophagous fungi and termites through vacuum-pressure impregnation. The test material was carefully selected and conditioned following the guidelines of EN 350:2016, and impregnation was carried out in accordance with EN 113-1:2021. Five concentrations of AgNPs–COS composites were utilized. Biodeterioration resistance was evaluated based on EN 350:2016 for white (Trametes versicolor (L.) Lloyd) and brown (Coniophora puteana (Schumach.) P.Karst.) rot fungi, and EN 117:2012 for subterranean termites (Reticulitermis grassei Clément). The durability class and use class were assigned following EN 350:2016 and EN 335:2013, respectively. In comparison to the untreated control, the binary solution at its highest concentration (AgNPs 4 ppm + COS 20 g·L⁻¹) demonstrated a notable reduction in weight loss, decreasing from 41.96 ± 4.49% to 30.15 ± 3.08% for white-rot fungi and from 41.93 ± 4.33% to 27.22 ± 0.66% for brown rot fungi. Furthermore, the observed termite infestation shifted from “heavy” to “attempted attack”, resulting in a decrease in the survival rate from 53.98 ± 10.40% to 26.62 ± 8.63%. Consequently, the durability classification of P. ×euramericana I-214 witnessed an enhancement from “Not durable” to “Slightly” and “Moderately durable” concerning decay fungi and termites, respectively. These findings expand the potential applications of this wood and substantiate the advantages of employing this environmentally friendly treatment.

Totora (Schoenoplectus californicus C.A. Mey. Soják) is a macrophyte from the Cyperaceae family that grows in the Americas. Recent studies have shown the feasibility of producing binderless boards using different tissues of totora stems. However, no data about the durability of raw totora stems or totora binderless boards have been published before. Therefore, durability tests were conducted to study their durability against wood-decaying organisms. Results showed that binderless boards produced by hot-pressing process showed higher resistance against white-rot fungi and termites than the raw material. Chemical changes induced by the hot-pressing process were studied and compared with observed durability values to identify possible correlations.

Aim of study: to assess the natural durability of Populus x euramericana ‘I-214’ against xylophagous fungi and termites, and to carry out a macro-microscopic analysis of the alterations caused by each xylophagous agent in order to get the necessary information for its possible inclusion in existing European standards.Area of study: A 20-years-old commercial plantation Populus x euramericana‘I-214’ located in Quintanilla de Sollamas (42° 36′ 00″N - 05° 49′ 00″ W), Spanish community of Castile-LeonMaterial and methods: material sampling and selection was carried out following EN 350:2017 for commercial sawn timber. Poplar resistance to xylophagous basidiomycete, soft rot fungi and subterranean termites was determined according to CEN/TS 15083-1:2005, CEN/TS 15083-2:2005 and EN 117:2012, respectively. The durability and use classes were estimated according to EN 350:2016 and EN 335:2013, respectively. The anatomical studies were carried out with Optical and Scanning Electron Microscope. Material characterization was carried out by reference to Anagnost (1998) and Schwarze (2007).Main results: ‘I-214’ poplar wood proved to be “Not-durable” to the action of basidiomycetes, soft rot fungi and termites, use classes 1-2, and showed macro-microscopic evidence of these types of decay.Research highlights: the information obtained in this study would allow the inclusion of clone I-214 in the standard EN 350 and its explicit classification within it.Keywords: wood-decay fungi; termites; EN 350.Abbreviations used: TM: test material; RM: reference material; RH: relative humidity; ML: mass loss; mi: initial dry mass; mf: final dry mass; DC: durability class; OM: Optical Microscope; SEM: Scanning Electron Microscope; TS: transverse section samples; LS: longitudinal section samples; CI: robust confidence intervals; F: fibre; V: vessel; h: hole; t: erosion trough; R: radial parenchyma cell; ep: erosion pitting; Fc: fungal colonization; fr: fracture; c: cavity; b: bore hole; m: mycelium.

Evaluating wood’s natural durability is essential when establishing the level of protection that is required depending on the end use to extend its service life. Natural durability is defined as the intrinsic resistance of wood against the attack of destructive organisms. There are standardized methods for estimating a durability value. In Europe, the EN 350:2016 standard is responsible for establishing the basic guidelines, as well as the necessary tests to determine this value. This standard applies to commercial wood, both native and imported, and helps to improve and obtain better construction applications depending on the final use. This work aimed to study the natural durability of Quercus pyrenaica Wild. against Basidiomycetes (Coniophora puteana (Schumacher ex Fries) Karsten and Trametes versicolor (Linnaeus) Quélet) and termites (Reticulitermes grassei Clément). The standards applied were EN 113-2:2021 and EN 117:2023. The heartwood of the Quercus pyrenaica is highly durable against both xylophages basidiomycetes and subterranean termites. The sapwood is moderately durable against Coniophora puteana, slightly durable-not durable against Trametes versicolor, and moderately durable against termites. These results open the door for commercialization of this species, and it is expected to be included in EN 350:2016, where Q. pyrenaica is not included.

The pine wood nematode Bursaphelenchus xylophilus (Nickle, 1970) is one of the most damaging pests affecting coniferous forests, prompting the European Union to adopt strict measures to limit its spread. In sawmills, wood is typically heat-treated following the International Standard for Phytosanitary Measures No. 15 (ISPM 15, 2019), which recommends heat treatment or fumigation with methyl bromide or sulfuryl fluoride for wood packaging materials including pallets, crates, and dunnage. To investigate safer and more cost-effective alternatives, this study uses ultrasound technology as a potential control method. Ultrasound, defined as high-frequency sound waves beyond human hearing, generates acoustic cavitation capable of damaging biological tissues. Nematodes were exposed to ultrasound both in vitro and within artificially inoculated green wood blocks (50 × 50 × 50 mm). In vitro tests showed significant reductions in viability at the measured time points (15, 30, 45 min), with complete mortality observed at 45 min. In wood blocks, nematode numbers declined progressively across the measured intervals, with linear regression providing a model-based estimate of complete elimination before approx. 80 min, although this was not experimentally verified. These results demonstrate that the use of ultrasound at 40 kHz reduces nematode survival under both laboratory conditions and wood matrix conditions.