To reveal the evolution of habitat distribution for multiple fish species in the lower reaches of the Gongzui Hydropower Station, this study conducted a catch survey to determine the target species of the reach. Based on their suitability curves, a combined suitability assessment model for multiple fish species was constructed. The reliability of the model was verified by combining acoustic observations of flow fields and fish distribution in specific flow conditions. A two-dimensional hydrodynamic model was coupled to quantitatively analyze the distribution characteristics of fish habitat patches under different flow conditions. The results indicate that the correlation coefficient between the multi-species comprehensive suitability index and the number of fish is 0.676, which indicates that the model can better evaluate the distribution of multiple fish habitats in the study river reach; the weighted usable area (WUA) decreased as the discharge increased; from low flow condition (2000 m³/s), the patch area of suitable habitat decreased from 11,424 m² to 1268 m², and the connectivity between patches also showed a downward trend; and the habitat shifted to the near-shore area of the downstream wider and shallower section, which was highly correlated with the migration process of low-depth and low-velocity areas. The model proposed in this study can establish a rapid response between the suitable habitat distribution of multiple fish species and discharge conditions, which can provide a research method for quantitative evaluation of multi-species habitats in river, and make a significant contribution to the sustainable development of riverine fisheries resources and river water ecology.

Visual cues are critical for orientation and migration in riverine fishes, providing potential mechanisms for behavioral guidance. This study investigated how light spectrum and intensity interact to modulate phototactic responses in two schizothoracine fishes from the upper Yalong River. Results showed Schizopygopsis malacanthus preferred blue light and avoided red light, with preferences shifting with flow intensity; Schizothorax kozlovi favored green light and avoided light-red light, with minimal flow impact. We propose that engineers build fishway entrances or ideal habitat attractors that prioritize low-intensity blue light (10 lx) and medium-intensity green light (50 lx), supplemented by medium-intensity blue light (50 lx). This study provides scientific evidence and application value for restoring fish habitats, fish passages, and fisheries.

To help fish to bypass dams and other human-made barriers, some fishways have ingeniously incorporated extended tunnel sections. This innovative design not only optimizes the overall structure of the fishway but also significantly reduces disturbances to the surrounding ecosystem. However, the potential challenges posed by long tunnel sections to fish upstream migration remain insufficiently studied and poorly understood. This study conducted in situ experiments utilizing a passive-integrated-transponder (PIT) system to quantitatively assess the effects of dark and natural light environments on the upstream migration behavior of plateau-endemic fishes (Schizothorax macropogon, Schizothorax waltoni, and Schizothorax oconnori) in a vertical-slot fishway. A 655 m section of the fishway was selected for the experiment, with shading cloth used to simulate the dark environment (DE) of tunnel sections, and its removal serving as the natural light environment (NE). The results showed that in the DE, the upstream behaviors of S. macropogon, S. waltoni, and S. oconnori were not hindered. The entry efficiency at the experimental segment (Ee) of all three species exceeded 65% in the DE, which was higher than that in the NE. The passage efficiency (Ep) of S. macropogon and S. waltoni showed no significant difference between the DE and NE, whereas S. oconnori exhibited a significant difference, with an overall Ep of 0% in the NE and 75.0% in the DE. Additionally, the DE caused a temporary disruption to the diel migration rhythms of the three species. The transit speeds (St) of S. macropogon and S. waltoni were both elevated in the DE, with S. waltoni showing a particularly significant increase; its average St in the DE (0.080 m/s) was much higher than in the NE (0.021 m/s). Ridge regression analysis further indicated that the DE was the primary factor influencing the St and had a positive effect on upstream behavior. Moreover, differences in the upstream migration performances of different species under varying light conditions highlighted species-specific sensitivity to light. This study offers key insights for fish passage design in canyon hydropower projects and highlights the potential of tunnel-type fishways in restoring river connectivity.