Minimizing the use of antibiotics in the food production chain is essential for limiting the development and spread of antibiotic-resistant bacteria. One alternative intervention strategy is the use of probiotic bacteria, and bacteria of the marine Roseobacter clade are capable of antagonizing fish-pathogenic vibrios in fish larvae and live feed cultures for fish larvae. The antibacterial compound tropodithietic acid (TDA), an antiporter that disrupts the proton motive force, is key in the antibacterial activity of several roseobacters. Introducing probiotics on a larger scale requires understanding of any potential side effects of long-term exposure of the pathogen to the probionts or any compounds they produce. Here we exposed the fish pathogen Vibrio anguillarum to TDA for several hundred generations in an adaptive evolution experiment. No tolerance or resistance arose during the 90 days of exposure, and whole-genome sequencing of TDA-exposed lineages and clones revealed few mutational changes, compared to lineages grown without TDA. Amino acid-changing mutations were found in two to six different genes per clone; however, no mutations appeared unique to the TDA-exposed lineages or clones. None of the virulence genes of V. anguillarum was affected, and infectivity assays using fish cell lines indicated that the TDA-exposed lineages and clones were less invasive than the wild-type strain. Thus, long-term TDA exposure does not appear to result in TDA resistance and the physiology of V. anguillarum appears unaffected, supporting the application of TDA-producing roseobacters as probiotics in aquaculture. IMPORTANCE It is important to limit the use of antibiotics in our food production, to reduce the risk of bacteria developing antibiotic resistance. We showed previously that marine bacteria of the Roseobacter clade can prevent or reduce bacterial diseases in fish larvae, acting as probiotics. Roseobacters produce the antimicrobial compound tropodithietic acid (TDA), and we were concerned regarding whether long-term exposure to this compound could induce resistance or affect the disease-causing ability of the fish pathogen. Therefore, we exposed the fish pathogen Vibrio anguillarum to increasing TDA concentrations over 3 months. We did not see the development of any resistance to TDA, and subsequent infection assays revealed that none of the TDA-exposed clones had increased virulence toward fish cells. Hence, this study supports the use of roseobacters as a non-risk-based disease control measure in aquaculture.

Bed shear stress is closely related to sediment transport in rivers. Bed shear stress estimation is very difficult, especially for complex flow fields. In this study, complex flow field measurement experiments in a 60° bend with a groyne were performed. The feasibility and reliability of bed shear stress estimations using the log-law method in a complex flow field were analyzed and compared with those associated with the Reynolds, Turbulent Kinetic Energy (TKE), and TKE-w′ methods. The results show that the TKE, Reynolds, and log-law methods produced similar bed shear stress estimates, while the TKE-w′ method produced larger estimates than the other methods. The TKE-w′ method was found to be more suitable for bed shear stress estimation than the TKE method, but the value of its constant C₂ needed to be re-estimated. In a complex, strong, three-dimensional flow field, the height of the measurement point (relative or absolute) should be re-estimated when a single point measurement is used to estimate the bed shear stress. The results of this study provide guidance for experimental measurement of bed shear stress in a complex flow field.

Spillway outlet design is a major issue in hydraulic engineering with high head and large discharge conditions. A new type of design for a streamwise-lateral spillway is proposed for ski-jump flow discharge and energy dissipation in hydraulic engineering. The water in the spillway outlet is constrained by three solid walls with an inclined floor, a horizontal floor on the bottom and a deflected side wall in the lateral direction. The water flow releases in a lateral direction into the plunge pool along the streamwise direction. It generates a free jet in the shape of “∩” in a limited area, causing the water to fully diffuse and stretch in the air simultaneously, and drop into the plunge pool to avoid excessive impact in the plunge pool. The formation mechanism for the flow pattern is analyzed, and the results show that the optimum inclination is an angle range of 30°~45° for a good performance of free ski-jump jet diffusion shape.

Groin type and vegetation in groin fields directly affect flow field, bank protection, and river evolution. Many studies focus on fixed-bed contexts, but there are few studies on the influence of riverbed changes on hydrodynamic characteristics around groins. In this study, three types of groins are investigated experimentally in fixed and mobile beds in terms of time-averaged flow characteristics, turbulence characteristics, and bed changes. In both fixed- and mobile-bed experiments, vegetation reduced erosion of the groin field and main stream. Compared with the fixed-bed experiment, the velocity in the main stream was decreased in the mobile-bed experiment, and the longitudinal turbulence intensity and lateral momentum exchange were increased. In this study, an improved three-dimensional groin group (upstream wing submerged T-shaped groin group) produced a lower sediment scouring capacity, average scour depth, and entrainment coefficient k than I-shaped and T-shaped groin groups.

Chute aerators introduce a large air discharge through air supply ducts to prevent cavitation erosion on spillways. There is not much information on the microcosmic air bubble characteristics near the chute bottom. This study was focused on examining the bottom air-water flow properties by performing a series of model tests that eliminated the upper aeration and illustrated the potential for bubble variation processes on the chute bottom. In comparison with the strong air detrainment in the impact zone, the bottom air bubble frequency decreased slightly. Observations showed that range of probability of the bubble chord length tended to decrease sharply in the impact zone and by a lesser extent in the equilibrium zone. A distinct mechanism to control the bubble size distribution, depending on bubble diameter, was proposed. For bubbles larger than about 1–2 mm, the bubble size distribution followed a—5/3 power-law scaling with diameter. Using the relationship between the local dissipation rate and bubble size, the bottom dissipation rate was found to increase along the chute bottom, and the corresponding Hinze scale showed a good agreement with the observations.