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Prof. Esther M. González
Universidad Pública de Navarra, Pamplona, Spain

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0 Carbon
0 Metabolism
0 ROOT
0 Drought
0 legumes

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Drought
Carbon
Metabolism
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Nitrogen Fixation
legumes

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Original research article
Published: 21 April 2021 in Frontiers in Plant Science
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Drought is an environmental stressor that affects crop yield worldwide. Understanding plant physiological responses to stress conditions is needed to secure food in future climate conditions. In this study, we applied a combination of plant physiology and metabolomic techniques to understand plant responses to progressive water deficit focusing on the root system. We chose two legume plants with contrasting tolerance to drought, the widely cultivated alfalfa Medicago sativa (Ms) and the model legume Medicago truncatula (Mt) for comparative analysis. Ms taproot (tapR) and Mt fibrous root (fibR) biomass increased during drought, while a progressive decline in water content was observed in both species. Metabolomic analysis allowed the identification of key metabolites in the different tissues tested. Under drought, carbohydrates, abscisic acid, and proline predominantly accumulated in leaves and tapRs, whereas flavonoids increased in fibRs in both species. Raffinose-family related metabolites accumulated during drought. Along with an accumulation of root sucrose in plants subjected to drought, both species showed a decrease in sucrose synthase (SUS) activity related to a reduction in the transcript level of SUS1, the main SUS gene. This study highlights the relevance of root carbon metabolism during drought conditions and provides evidence on the specific accumulation of metabolites throughout the root system.

ACS Style

Andres Echeverria; Estíbaliz Larrainzar; Weiqiang Li; Yasuko Watanabe; Muneo Sato; Cuong Duy Tran; Jose A. Moler; Masami Yokota Hirai; Yuji Sawada; Lam-Son Phan Tran; Esther M. Gonzalez. Medicago sativa and Medicago truncatula Show Contrasting Root Metabolic Responses to Drought. Frontiers in Plant Science 2021, 12, 1 .

AMA Style

Andres Echeverria, Estíbaliz Larrainzar, Weiqiang Li, Yasuko Watanabe, Muneo Sato, Cuong Duy Tran, Jose A. Moler, Masami Yokota Hirai, Yuji Sawada, Lam-Son Phan Tran, Esther M. Gonzalez. Medicago sativa and Medicago truncatula Show Contrasting Root Metabolic Responses to Drought. Frontiers in Plant Science. 2021; 12 ():1.

Chicago/Turabian Style

Andres Echeverria; Estíbaliz Larrainzar; Weiqiang Li; Yasuko Watanabe; Muneo Sato; Cuong Duy Tran; Jose A. Moler; Masami Yokota Hirai; Yuji Sawada; Lam-Son Phan Tran; Esther M. Gonzalez. 2021. "Medicago sativa and Medicago truncatula Show Contrasting Root Metabolic Responses to Drought." Frontiers in Plant Science 12, no. : 1.

Regular article
Published: 18 March 2021 in Plant and Soil
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Here, we assess the differential impact of drought on root carbon metabolism in the widely cultivated alfalfa (Medicago sativa, Ms) and the model legume Medicago truncatula (Mt). Understanding how carbon allocation is regulated under drought stress conditions is a central issue to improving alfalfa productivity under future climate change scenarios. Alfalfa and Medicago truncatula were compared under water deficit conditions. Root carbon metabolism of the taproot and fibrous roots was analysed. M. truncatula drought tolerance variability was compared to that of alfalfa using six accessions of the Medicago Hapmap project. The prominent taproot is much less developed in M. truncatula than in alfalfa with the former exhibiting an extensive fibrous root system. In both examined Medicago species the taproot contained the major pools of soluble protein, sucrose and pinitol, whereas the major pools of hexoses and carbon metabolism enzymes appeared to be in the fibrous roots. Under water-deficit conditions, the response of M. sativa strongly differed from that of M. truncatula at the root level. Water deficit conditions differentially modulate the root carbon metabolism of M. sativa and M. truncatula. Mt maintained a more active carbon metabolism in the fibRs, as sucrose, myo-inositol and pinitol accumulated to cope with the water deficit (WD). Conversely, the root system of Ms did not accumulate cyclitols and carbon metabolism was more severely affected under water deficit conditions. This differentially exerted control may determine the drought response of these two close relatives.

ACS Style

Andres Echeverria; Esther M. Gonzalez. Root system of Medicago sativa and Medicago truncatula: drought effects on carbon metabolism. Plant and Soil 2021, 463, 249 -263.

AMA Style

Andres Echeverria, Esther M. Gonzalez. Root system of Medicago sativa and Medicago truncatula: drought effects on carbon metabolism. Plant and Soil. 2021; 463 (1-2):249-263.

Chicago/Turabian Style

Andres Echeverria; Esther M. Gonzalez. 2021. "Root system of Medicago sativa and Medicago truncatula: drought effects on carbon metabolism." Plant and Soil 463, no. 1-2: 249-263.

Journal article
Published: 10 March 2021 in International Journal of Molecular Sciences
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Water-deficit stresses such as drought and salinity are the most important factors limiting crop productivity. Hence, understanding the plant responses to these stresses is key for the improvement of their tolerance and yield. In this study M. truncatula plants were subjected to 250 mM NaCl as well as reduced irrigation (No-W) and 250 g/L polyethylene glycol (PEG)-6000 to induce salinity and drought stress, respectively, provoking a drop to −1.7 MPa in leaf water potential. The whole plant physiology and metabolism was explored by characterizing the stress responses at root, phloem sap and leaf organ level. PEG treatment led to some typical responses of plants to drought stress, but in addition to PEG uptake, an important impairment of nutrient uptake and a different regulation of carbon metabolism could be observed compared to No-W plants. No-W plants showed an important redistribution of antioxidants and assimilates to the root tissue, with a distinctive increase in root proline degradation and alkaline invertase activity. On the contrary, salinity provoked an increase in leaf starch and isocitrate dehydrogenase activity, suggesting key roles in the plant response to this stress. Overall, results suggest higher protection of salt-stressed shoots and non-irrigated roots through different mechanisms, including the regulation of proline and carbon metabolism, while discarding PEG as safe mimicker of drought. This raises the need to understand the effect at the whole plant level of the different strategies employed to apply water-deficit stress.

ACS Style

Verónica Castañeda; Esther González. Strategies to Apply Water-Deficit Stress: Similarities and Disparities at the Whole Plant Metabolism Level in Medicago truncatula. International Journal of Molecular Sciences 2021, 22, 2813 .

AMA Style

Verónica Castañeda, Esther González. Strategies to Apply Water-Deficit Stress: Similarities and Disparities at the Whole Plant Metabolism Level in Medicago truncatula. International Journal of Molecular Sciences. 2021; 22 (6):2813.

Chicago/Turabian Style

Verónica Castañeda; Esther González. 2021. "Strategies to Apply Water-Deficit Stress: Similarities and Disparities at the Whole Plant Metabolism Level in Medicago truncatula." International Journal of Molecular Sciences 22, no. 6: 2813.

Original research article
Published: 02 February 2021 in Frontiers in Plant Science
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During moderate drought stress, plants can adjust by changes in the protein profiles of the different organs. Plants transport and modulate extracellular stimuli local and systemically through commonly induced inter- and intracellular reactions. However, most proteins are frequently considered, cell and organelle specific. Hence, while signaling molecules and peptides can travel systemically throughout the whole plant, it is not clear, whether protein isoforms may exist ubiquitously across organs, and what function those may have during drought regulation. By applying shotgun proteomics, we extracted a core proteome of 92 identical protein isoforms, shared ubiquitously amongst several Medicago truncatula tissues, including roots, phloem sap, petioles, and leaves. We investigated their relative distribution across the different tissues and their response to moderate drought stress. In addition, we functionally compared this plant core stress responsive proteome with the organ-specific proteomes. Our study revealed plant ubiquitous protein isoforms, mainly related to redox homeostasis and signaling and involved in protein interaction networks across the whole plant. Furthermore, about 90% of these identified core protein isoforms were significantly involved in drought stress response, indicating a crucial role of the core stress responsive proteome (CSRP) in the plant organ cross-communication, important for a long-distance stress-responsive network. Besides, the data allowed for a comprehensive characterization of the phloem proteome, revealing new insights into its function. For instance, CSRP protein levels involved in stress and redox are relatively more abundant in the phloem compared to the other tissues already under control conditions. This suggests a major role of the phloem in stress protection and antioxidant activity enabling the plants metabolic maintenance and rapid response upon moderate stress. We anticipate our study to be a starting point for future investigations of the role of the core plant proteome. Under an evolutionary perspective, CSRP would enable communication of different cells with each other and the environment being crucial for coordinated stress response of multicellular organisms.

ACS Style

Veronica Castañeda; Esther M. González; Stefanie Wienkoop. Phloem Sap Proteins Are Part of a Core Stress Responsive Proteome Involved in Drought Stress Adjustment. Frontiers in Plant Science 2021, 12, 1 .

AMA Style

Veronica Castañeda, Esther M. González, Stefanie Wienkoop. Phloem Sap Proteins Are Part of a Core Stress Responsive Proteome Involved in Drought Stress Adjustment. Frontiers in Plant Science. 2021; 12 ():1.

Chicago/Turabian Style

Veronica Castañeda; Esther M. González; Stefanie Wienkoop. 2021. "Phloem Sap Proteins Are Part of a Core Stress Responsive Proteome Involved in Drought Stress Adjustment." Frontiers in Plant Science 12, no. : 1.

Journal article
Published: 14 January 2021 in Sustainability
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Biodiversity is a concept of great scientific interest and social value studied in different subjects of the secondary education curriculum. Citizen–science programs may contribute to increasing the engagement of students when studying biodiversity. This work aimed to explore the use of the citizen–science platform iNaturalist as a complement of the elaboration of herbaria in an outdoor activity for 4th course 16-year-old students in the Basaula Reserve. The platform iNaturalist was chosen for its suitability to develop collaborative projects in an educational context. The Basaula project was created and 122 students were trained to record plant species in an outdoor activity. A total of 32 species were recorded, among them the most abundant were beech (Fagus sylvatica) and holm oak (Quercus ilex). The students positively evaluated their experience, highlighting its adequacy to record biodiversity data and make a virtual herbarium. Students valued the innovative character of iNaturalist and its usefulness for research but also the opportunity to integrate mobile devices in school education. We concluded that iNaturalist is a valuable tool to carry out collaborative projects dealing with biodiversity in secondary education.

ACS Style

Andres Echeverria; Idoia Ariz; Judit Moreno; Javier Peralta; Esther Gonzalez. Learning Plant Biodiversity in Nature: The Use of the Citizen–Science Platform iNaturalist as a Collaborative Tool in Secondary Education. Sustainability 2021, 13, 735 .

AMA Style

Andres Echeverria, Idoia Ariz, Judit Moreno, Javier Peralta, Esther Gonzalez. Learning Plant Biodiversity in Nature: The Use of the Citizen–Science Platform iNaturalist as a Collaborative Tool in Secondary Education. Sustainability. 2021; 13 (2):735.

Chicago/Turabian Style

Andres Echeverria; Idoia Ariz; Judit Moreno; Javier Peralta; Esther Gonzalez. 2021. "Learning Plant Biodiversity in Nature: The Use of the Citizen–Science Platform iNaturalist as a Collaborative Tool in Secondary Education." Sustainability 13, no. 2: 735.

Journal article
Published: 01 August 2018 in Agricultural Water Management
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Root performance represents a target factor conditioning plant development under drought conditions. Moreover, recent root phenotyping studies remark relevant differences on functionality of the different root types. However, despite its relevance, the performance of different types of roots such as primary/taproot (tapR) and lateral/fibrous roots (fibR) under water stress conditions is largely unknown. In the current study, the impact of water stress on target C and N metabolism (namely sucrose and proline) processes were characterized in tapR and fibR of Medicago truncatula plants exposed to different water stress severity regimes (moderate versus severe). While both root types exhibit some common responses to face water stress, the study highlighted important physiological and metabolic differences between them. The tapR proved to have an essential role on carbon and nitrogen partitioning rather than just on storage. Moreover, this root type showed a higher resilience towards water deficit stress. Sucrose metabolization at sucrose synthase level was early blocked in this tissue together with a selective accumulation of some amino acids such as proline and branched chain amino acids, which may act as alternative carbon sources under water deficit stress conditions. The decline in respiration, despite the over-accumulation of carbon compounds, suggests a modulation at sucrose cleavage level by sucrose synthase and invertase. These data not only provide new information on the carbon and nitrogen metabolism modulation upon water deficit stress but also on the different role, physiology, and metabolism of the taproot and fibrous roots. In addition, obtained results highlight the fact that both root types show distinct performance under water deficit stress; this factor can be of great relevance to improve breeding programs for increasing root efficiency under adverse conditions.

ACS Style

Veronica Castañeda; Marlon de la Peña; Lidia Azcárate; Iker Aranjuelo; Esther M. Gonzalez. Functional analysis of the taproot and fibrous roots of Medicago truncatula: Sucrose and proline catabolism primary response to water deficit. Agricultural Water Management 2018, 216, 473 -483.

AMA Style

Veronica Castañeda, Marlon de la Peña, Lidia Azcárate, Iker Aranjuelo, Esther M. Gonzalez. Functional analysis of the taproot and fibrous roots of Medicago truncatula: Sucrose and proline catabolism primary response to water deficit. Agricultural Water Management. 2018; 216 ():473-483.

Chicago/Turabian Style

Veronica Castañeda; Marlon de la Peña; Lidia Azcárate; Iker Aranjuelo; Esther M. Gonzalez. 2018. "Functional analysis of the taproot and fibrous roots of Medicago truncatula: Sucrose and proline catabolism primary response to water deficit." Agricultural Water Management 216, no. : 473-483.

Chapter
Published: 29 July 2018 in Engineering Nitrogen Utilization in Crop Plants
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Legumes account for around 27% of the world’s primary crop production and can be classified based on their use and traits into grain and forage legumes. Legumes can establish symbiosis with N-fixing soil bacteria. As a result, a new organ is formed, the nodule, where the reduction of atmospheric N2 into ammonia is carried out catalyzed by the bacterial exclusive enzyme nitrogenase. The process, highly energy demanding, is known as symbiotic nitrogen fixation and provides all the N needs of the plant, thus avoiding the use of N fertilizers in the context of sustainable agriculture. However, legume crops are often grown under non-fixing conditions since legume nodulation is suppressed by high levels of soil nitrogen occurring in chemically fertilized agro-environment. In addition, legumes are very sensitive to environmental stresses, being drought one of the significant constraints affecting crop production. Due to their agricultural and economic importance, scientists have carried out basic and applied research on legumes to better understand responses to abiotic stresses and to further comprehend plant–microbe interactions. An integrated view of nitrogen utilization under drought stress will be presented with particular focus on legume crops.

ACS Style

V. Castañeda; E. Gil-Quintana; A. Echeverria; Em. González. Legume Nitrogen Utilization Under Drought Stress. Engineering Nitrogen Utilization in Crop Plants 2018, 173 -184.

AMA Style

V. Castañeda, E. Gil-Quintana, A. Echeverria, Em. González. Legume Nitrogen Utilization Under Drought Stress. Engineering Nitrogen Utilization in Crop Plants. 2018; ():173-184.

Chicago/Turabian Style

V. Castañeda; E. Gil-Quintana; A. Echeverria; Em. González. 2018. "Legume Nitrogen Utilization Under Drought Stress." Engineering Nitrogen Utilization in Crop Plants , no. : 173-184.

Journal article
Published: 01 July 2018 in Ecological Informatics
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Acoustic bat identification is a complementary method to traditional mist-netting for chiropteran surveys. In this work, an algorithm based on artificial intelligence techniques was developed for bat species identification in Uruguay. An acoustic library of 662 search phase pulses of the 10 most common bat species in Uruguay was obtained. Random Forest, Support Vector Machines and Artificial Neural Networks algorithms were trained to predict bat species from acoustic variables. Variable selection was performed in an independent subset (one third) of the dataset, using randomForest R package. Model performance was evaluated by means of the generalization error with the remaining two thirds of the data. To do this, data were split randomly into a training and a test sets, then the model was trained with the training sample and its performance was assessed using the test sample. The procedure was performed 100 times and the generalization errors were averaged to have an unbiased measure of the performance of the models. The best predictor was a Random Forests classifier that considered 12 predictor variables. The achieved accuracy was comparable to other international published products. Additionally, a threshold value for classification probability was optimized to define an “unclassified” class that allows using this algorithm even when the training sample does not represent an exhaustive sample of local richness. A web application returning the predicted class and a confidence measure for a given observation was developed permitting the use of this tool by a broad spectrum of users, from biologist to technicians.

ACS Style

G. Botto Nuñez; G. Lemus; M. Muñoz Wolf; A.L. Rodales; E.M. González; C. Crisci. The first artificial intelligence algorithm for identification of bat species in Uruguay. Ecological Informatics 2018, 46, 97 -102.

AMA Style

G. Botto Nuñez, G. Lemus, M. Muñoz Wolf, A.L. Rodales, E.M. González, C. Crisci. The first artificial intelligence algorithm for identification of bat species in Uruguay. Ecological Informatics. 2018; 46 ():97-102.

Chicago/Turabian Style

G. Botto Nuñez; G. Lemus; M. Muñoz Wolf; A.L. Rodales; E.M. González; C. Crisci. 2018. "The first artificial intelligence algorithm for identification of bat species in Uruguay." Ecological Informatics 46, no. : 97-102.

Original research article
Published: 15 June 2017 in Frontiers in Plant Science
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Drought provokes a number of physiological changes in plants including oxidative damage. Ascorbic acid (AsA), also known as vitamin C, is one of the most abundant water-soluble antioxidant compound present in plant tissues. However, little is known on the regulation of AsA biosynthesis under drought stress conditions. In the current work we analyze the effects of water deficit on the biosynthesis of AsA by measuring its content, in vivo biosynthesis and the expression level of genes in the Smirnoff-Wheeler pathway in one of the major legume crop, soybean (Glycine max L. Merr). Since the pathway has not been described in legumes, we first searched for the putative orthologous genes in the soybean genome. We observed a significant genetic redundancy, with multiple genes encoding each step in the pathway. Based on RNA-seq analysis, expression of the complete pathway was detected not only in leaves but also in root tissue. Putative paralogous genes presented differential expression patterns in response to drought, suggesting the existence of functional specialization mechanisms. We found a correlation between the levels of AsA and GalLDH biosynthetic rates in leaves of drought-stressed soybean plants. However, the levels of GalLDH transcripts did not show significant differences under water deficit conditions. Among the other known regulators of the pathway, only the expression of VTC1 genes correlated with the observed decline in AsA in leaves.

ACS Style

Amaia Seminario; Li Song; Amaia Zulet; Henry T. Nguyen; Esther M. González; Estíbaliz Larrainzar. Drought Stress Causes a Reduction in the Biosynthesis of Ascorbic Acid in Soybean Plants. Frontiers in Plant Science 2017, 8, 1042 -1042.

AMA Style

Amaia Seminario, Li Song, Amaia Zulet, Henry T. Nguyen, Esther M. González, Estíbaliz Larrainzar. Drought Stress Causes a Reduction in the Biosynthesis of Ascorbic Acid in Soybean Plants. Frontiers in Plant Science. 2017; 8 ():1042-1042.

Chicago/Turabian Style

Amaia Seminario; Li Song; Amaia Zulet; Henry T. Nguyen; Esther M. González; Estíbaliz Larrainzar. 2017. "Drought Stress Causes a Reduction in the Biosynthesis of Ascorbic Acid in Soybean Plants." Frontiers in Plant Science 8, no. : 1042-1042.

Clinical trial
Published: 12 September 2016 in Breast Cancer Research and Treatment
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Improved therapies and imaging modalities are needed for the treatment of breast cancer brain metastases (BCBM). ANG1005 is a drug conjugate consisting of paclitaxel covalently linked to Angiopep-2, designed to cross the blood-brain barrier. We conducted a biomarker substudy to evaluate 18F-FLT-PET for response assessment. Ten patients with measurable BCBM received ANG1005 at a dose of 550 mg/m2 IV every 21 days. Before and after cycle 1, patients underwent PET imaging with 18F-FLT, a thymidine analog, retention of which reflects cellular proliferation, for comparison with gadolinium-contrast magnetic resonance imaging (Gd-MRI) in brain metastases detection and response assessment. A 20 % change in uptake after one cycle of ANG1005 was deemed significant. Thirty-two target and twenty non-target metastatic brain lesions were analyzed. The median tumor reduction by MRI after cycle 1 was -17.5 % (n = 10 patients, lower, upper quartiles: -25.5, -4.8 %) in target lesion size compared with baseline. Fifteen of twenty-nine target lesions (52 %) and 12/20 nontarget lesions (60 %) showed a ≥20 % decrease post-therapy in FLT-PET SUV change (odds ratio 0.71, 95 % CI: 0.19, 2.61). The median percentage change in SUVmax was -20.9 % (n = 29 lesions; lower, upper quartiles: -42.4, 2.0 %), and the median percentage change in SUV80 was also -20.9 % (n = 29; lower, upper quartiles: -49.0, 0.0 %). Two patients had confirmed partial responses by PET and MRI lasting 6 and 18 cycles, respectively. Seven patients had stable disease, receiving a median of six cycles. ANG1005 warrants further study in BCBM. Results demonstrated a moderately strong association between MRI and 18F-FLT-PET imaging.

ACS Style

C. C. O’Sullivan; M. Lindenberg; C. Bryla; N. Patronas; C. J. Peer; L. Amiri-Kordestani; N. Davarpanah; E. M. Gonzalez; M. Burotto; P. Choyke; S. M. Steinberg; D. J. Liewehr; W. D. Figg; T. Fojo; Sanjeeve Balasubramaniam; S. E. Bates. ANG1005 for breast cancer brain metastases: correlation between 18F-FLT-PET after first cycle and MRI in response assessment. Breast Cancer Research and Treatment 2016, 160, 51 -59.

AMA Style

C. C. O’Sullivan, M. Lindenberg, C. Bryla, N. Patronas, C. J. Peer, L. Amiri-Kordestani, N. Davarpanah, E. M. Gonzalez, M. Burotto, P. Choyke, S. M. Steinberg, D. J. Liewehr, W. D. Figg, T. Fojo, Sanjeeve Balasubramaniam, S. E. Bates. ANG1005 for breast cancer brain metastases: correlation between 18F-FLT-PET after first cycle and MRI in response assessment. Breast Cancer Research and Treatment. 2016; 160 (1):51-59.

Chicago/Turabian Style

C. C. O’Sullivan; M. Lindenberg; C. Bryla; N. Patronas; C. J. Peer; L. Amiri-Kordestani; N. Davarpanah; E. M. Gonzalez; M. Burotto; P. Choyke; S. M. Steinberg; D. J. Liewehr; W. D. Figg; T. Fojo; Sanjeeve Balasubramaniam; S. E. Bates. 2016. "ANG1005 for breast cancer brain metastases: correlation between 18F-FLT-PET after first cycle and MRI in response assessment." Breast Cancer Research and Treatment 160, no. 1: 51-59.

Journal article
Published: 01 March 2016 in Journal of Proteomics
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Drought stress hampers plant energy and biomass production; however it is still unknown how internal C:N balance and rhizobial symbiosis impact on plant response to water limitation. Here, the effect of differential optimal nitrogen nutrition and root nodule symbiosis on drought stress and rehydration responses of Medicago truncatula was assessed. Two groups of plants were nodulated with Sinorhizobium medicae or Sinorhizobiummeliloti — differing in the performance of N fixation; the third group grew in a rhizobia-free medium and received mineral nitrogen fertilizer. In addition to growth analyses, physiological and molecular responses of the two systems were studied using ionomic, metabolomic and proteomic techniques. We found a significant delay in drought-induced leaf senescence in nodulated relative to non-nodulated plants, independent of rhizobial strain and uncoupled from initial leaf N content. The major mechanisms involved are increased concentrations of potassium and shifts in the carbon partitioning between starch and sugars under well-watered conditions, as well as the enhanced allocation of reserves to osmolytes during drought. Consequently, nodulated plants recovered more effectively from drought, relative to non-nodulated M. truncatula. Proteomic data suggest that phytohormone interactions and enhanced translational regulation play a role in increased leaf maintenance in nodulated plants during drought.

ACS Style

Christiana Staudinger; Vlora Mehmeti-Tershani; Erena Gil-Quintana; Esther M. Gonzalez; Florian Hofhansl; Gert Bachmann; Stefanie Wienkoop. Evidence for a rhizobia-induced drought stress response strategy in Medicago truncatula. Journal of Proteomics 2016, 136, 202 -213.

AMA Style

Christiana Staudinger, Vlora Mehmeti-Tershani, Erena Gil-Quintana, Esther M. Gonzalez, Florian Hofhansl, Gert Bachmann, Stefanie Wienkoop. Evidence for a rhizobia-induced drought stress response strategy in Medicago truncatula. Journal of Proteomics. 2016; 136 ():202-213.

Chicago/Turabian Style

Christiana Staudinger; Vlora Mehmeti-Tershani; Erena Gil-Quintana; Esther M. Gonzalez; Florian Hofhansl; Gert Bachmann; Stefanie Wienkoop. 2016. "Evidence for a rhizobia-induced drought stress response strategy in Medicago truncatula." Journal of Proteomics 136, no. : 202-213.

Comparative study
Published: 06 November 2015 in Journal of Proteome Research
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Legume crops present important agronomical and environmental advantages mainly due to their capacity to reduce atmospheric N2 to ammonium via symbiotic nitrogen fixation (SNF). This process is very sensitive to abiotic stresses such as drought, but the mechanism underlying this response is not fully understood. The goal of the current work is to compare the drought response of two legumes with high economic impact and research importance, Medicago truncatula and Glycine max, by characterizing their root nodule proteomes. Our results show that, although M. truncatula exhibits lower water potential values under drought conditions compared to G. max, SNF declined analogously in the two legumes. Both of their nodule proteomes are very similar, and comparable down-regulation responses in the diverse protein functional groups were identified (mainly proteins related to the metabolism of carbon, nitrogen, and sulfur). We suggest lipoxygenases and protein turnover as newly recognized players in SNF regulation. Partial drought conditions applied to a split-root system resulted in the local down-regulation of the entire proteome of drought-stressed nodules in both legumes. The high degree of similarity between both legume proteomes suggests that the vast amount of research conducted on M. truncatula could be applied to economically important legume crops, such as soybean.

ACS Style

Erena Gil-Quintana; David Lyon; Christiana Staudinger; Stefanie Wienkoop; Esther M. González. Medicago truncatula and Glycine max: Different Drought Tolerance and Similar Local Response of the Root Nodule Proteome. Journal of Proteome Research 2015, 14, 5240 -5251.

AMA Style

Erena Gil-Quintana, David Lyon, Christiana Staudinger, Stefanie Wienkoop, Esther M. González. Medicago truncatula and Glycine max: Different Drought Tolerance and Similar Local Response of the Root Nodule Proteome. Journal of Proteome Research. 2015; 14 (12):5240-5251.

Chicago/Turabian Style

Erena Gil-Quintana; David Lyon; Christiana Staudinger; Stefanie Wienkoop; Esther M. González. 2015. "Medicago truncatula and Glycine max: Different Drought Tolerance and Similar Local Response of the Root Nodule Proteome." Journal of Proteome Research 14, no. 12: 5240-5251.

Book chapter
Published: 01 January 2015 in Legume Nitrogen Fixation in a Changing Environment
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A significant decline in the content of water in soils provokes a water deficit at the plant level. In plant physiology, water deficit can be defined as the water content of a tissue or cell below the highest water content under the optimum hydrated state. The basis of the fundamental mechanism involved in stress tolerance, although intensively explored, is still matter of debate. Cell growth is the physiological process first affected as cell water content decreases when plants encounter mild water-deficit levels, followed by an inhibition of cell wall and protein biosynthesis. Although stomatal conductance and photosynthesis are affected in more intense water-deficit stages, most research efforts have focused on the study of these processes. In legume plants grown under symbiotic conditions, one of the primary effects of water deficit is a decline in the rates of symbiotic nitrogen fixation (SNF). The causes of this inhibition, which occurs even before a measurable decline in the rates of photosynthesis, have been explored in detail in the last decades, although the molecular mechanism involved are yet not fully understood. In the present chapter, we summarize our current understanding of the factors involved in the regulation of SNF in different legume species, including crops such as soybean (Glycine max), alfalfa (Medicago sativa), bean (Phaseolus vulgaris), and pea (Pisum sativum) but also model legumes like Medicago truncatula. Finally, an overview of the available resources and applications of molecular system-based approaches for understanding the complex responses of legumes to drought stress is provided.

ACS Style

Esther M. González; Estibaliz Larrainzar; Daniel Marino; Stefanie Wienkoop; Erena Gil-Quintana; Cesar Arrese-Igor. Physiological Responses of N2-Fixing Legumes to Water Limitation. Legume Nitrogen Fixation in a Changing Environment 2015, 5 -33.

AMA Style

Esther M. González, Estibaliz Larrainzar, Daniel Marino, Stefanie Wienkoop, Erena Gil-Quintana, Cesar Arrese-Igor. Physiological Responses of N2-Fixing Legumes to Water Limitation. Legume Nitrogen Fixation in a Changing Environment. 2015; ():5-33.

Chicago/Turabian Style

Esther M. González; Estibaliz Larrainzar; Daniel Marino; Stefanie Wienkoop; Erena Gil-Quintana; Cesar Arrese-Igor. 2015. "Physiological Responses of N2-Fixing Legumes to Water Limitation." Legume Nitrogen Fixation in a Changing Environment , no. : 5-33.

Journal article
Published: 30 December 2014 in Revista Lugares de Educação
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ACS Style

M.L. Gonzalez; E.M. González. La Alternativa para la Producción de Conocimientos en Trabajo Social: Un Miramiento desde la Universidad Venezolana. Revista Lugares de Educação 2014, 4, 83 -97.

AMA Style

M.L. Gonzalez, E.M. González. La Alternativa para la Producción de Conocimientos en Trabajo Social: Un Miramiento desde la Universidad Venezolana. Revista Lugares de Educação. 2014; 4 (9):83-97.

Chicago/Turabian Style

M.L. Gonzalez; E.M. González. 2014. "La Alternativa para la Producción de Conocimientos en Trabajo Social: Un Miramiento desde la Universidad Venezolana." Revista Lugares de Educação 4, no. 9: 83-97.

Original research article
Published: 27 August 2014 in Frontiers in Microbiology
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The symbiotic association between Medicago truncatula and Sinorhizobium meliloti is a well-established model system in the legume-Rhizobium community. Despite its wide use, the symbiotic efficiency of this model has been recently questioned and an alternative microsymbiont, S. medicae, has been proposed. However, little is known about the physiological mechanisms behind the higher symbiotic efficiency of S. medicae WSM419. In the present study, we inoculated M. truncatula Jemalong A17 with either S. medicae WSM419 or S. meliloti 2011 and compared plant growth, photosynthesis, N2-fixation rates, and plant nodule carbon and nitrogen metabolic activities in the two systems. M. truncatula plants in symbiosis with S. medicae showed increased biomass and photosynthesis rates per plant. Plants grown in symbiosis with S. medicae WSM419 also showed higher N2-fixation rates, which were correlated with a larger nodule biomass, while nodule number was similar in both systems. In terms of plant nodule metabolism, M. truncatula-S. medicae WSM419 nodules showed increased sucrose-catabolic activity, mostly associated with sucrose synthase, accompanied by a reduced starch content, whereas nitrogen-assimilation activities were comparable to those measured in nodules infected with S. meliloti 2011. Taken together, these results suggest that S. medicae WSM419 is able to enhance plant carbon catabolism in M. truncatula nodules, which allows for the maintaining of high symbiotic N2-fixation rates, better growth and improved general plant performance.

ACS Style

Estibaliz Larrainzar; Erena Egil-Quintana; Amaia Seminario; Cesar Arrese-Igor; Esther M. Gonzã¡lez. Nodule carbohydrate catabolism is enhanced in the Medicago truncatula A17-Sinorhizobium medicae WSM419 symbiosis. Frontiers in Microbiology 2014, 5, 447 .

AMA Style

Estibaliz Larrainzar, Erena Egil-Quintana, Amaia Seminario, Cesar Arrese-Igor, Esther M. Gonzã¡lez. Nodule carbohydrate catabolism is enhanced in the Medicago truncatula A17-Sinorhizobium medicae WSM419 symbiosis. Frontiers in Microbiology. 2014; 5 ():447.

Chicago/Turabian Style

Estibaliz Larrainzar; Erena Egil-Quintana; Amaia Seminario; Cesar Arrese-Igor; Esther M. Gonzã¡lez. 2014. "Nodule carbohydrate catabolism is enhanced in the Medicago truncatula A17-Sinorhizobium medicae WSM419 symbiosis." Frontiers in Microbiology 5, no. : 447.

Minireview
Published: 26 June 2014 in Journal of Integrative Plant Biology
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Split‐root system (SRS) approaches allow the differential treatment of separate and independent root systems, while sharing a common aerial part. As such, SRS is a useful tool for the discrimination of systemic (shoot origin) versus local (root/nodule origin) regulation mechanisms. This type of approach is particularly useful when studying the complex regulatory mechanisms governing the symbiosis established between legumes and Rhizobium bacteria. The current work provides an overview of the main insights gained from the application of SRS approaches to understand how nodule number (nodulation autoregulation) and nitrogen fixation are controlled both under non‐stressful conditions and in response to a variety of stresses. Nodule number appears to be mainly controlled at the systemic level through a signal which is produced by nodule/root tissue, translocated to the shoot, and transmitted back to the root system, involving shoot Leu‐rich repeat receptor‐like kinases. In contrast, both local and systemic mechanisms have been shown to operate for the regulation of nitrogenase activity in nodules. Under drought and heavy metal stress, the regulation is mostly local, whereas the application of exogenous nitrogen seems to exert a regulation of nitrogen fixation both at the local and systemic levels.

ACS Style

Estibaliz Larrainzar; Erena Gil-Quintana; Cesar Arrese-Igor; Esther M. González; Daniel Marino. Split-root systems applied to the study of the legume-rhizobial symbiosis: What have we learned? Journal of Integrative Plant Biology 2014, 56, 1118 -1124.

AMA Style

Estibaliz Larrainzar, Erena Gil-Quintana, Cesar Arrese-Igor, Esther M. González, Daniel Marino. Split-root systems applied to the study of the legume-rhizobial symbiosis: What have we learned? Journal of Integrative Plant Biology. 2014; 56 (12):1118-1124.

Chicago/Turabian Style

Estibaliz Larrainzar; Erena Gil-Quintana; Cesar Arrese-Igor; Esther M. González; Daniel Marino. 2014. "Split-root systems applied to the study of the legume-rhizobial symbiosis: What have we learned?" Journal of Integrative Plant Biology 56, no. 12: 1118-1124.

Original article
Published: 23 April 2014 in Physiologia Plantarum
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Drought is considered the more harmful abiotic stress resulting in crops yield loss. Legumes in symbiosis with rhizobia are able to fix atmospheric nitrogen. Biological nitrogen fixation (SNF) is a very sensitive process to drought and limits legumes agricultural productivity. Several factors are known to regulate SNF including oxygen availability to bacteroids, carbon and nitrogen metabolisms; but the signaling pathways leading to SNF inhibition are largely unknown. In this work, we have performed a proteomic approach of pea plants grown in split‐root system where one half of the root was well‐irrigated and the other was subjected to drought. Water stress locally provoked nodule water potential decrease that led to SNF local inhibition. The proteomic approach revealed 11 and 7 nodule proteins regulated by drought encoded by Pisum sativum and Rhizobium leguminosarum genomes respectively. Among these 18 proteins, 3 proteins related to flavonoid metabolism, 2 to sulfur metabolism and 3 RNA‐binding proteins were identified. These proteins could be molecular targets for future studies focused on the improvement of legumes tolerance to drought. Moreover, this work also provides new hints for the deciphering of SNF regulation machinery in nodules.

ACS Style

Sami Irar; Esther M. González; Cesar Arrese-Igor; Daniel Marino. A proteomic approach reveals new actors of nodule response to drought in split-root grown pea plants. Physiologia Plantarum 2014, 152, 634 -645.

AMA Style

Sami Irar, Esther M. González, Cesar Arrese-Igor, Daniel Marino. A proteomic approach reveals new actors of nodule response to drought in split-root grown pea plants. Physiologia Plantarum. 2014; 152 (4):634-645.

Chicago/Turabian Style

Sami Irar; Esther M. González; Cesar Arrese-Igor; Daniel Marino. 2014. "A proteomic approach reveals new actors of nodule response to drought in split-root grown pea plants." Physiologia Plantarum 152, no. 4: 634-645.

Original article
Published: 29 January 2014 in Plant, Cell & Environment
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Symbiotic nitrogen fixation is one of the first physiological processes inhibited in legume plants under water‐deficit conditions. Despite the progress made in the last decades, the molecular mechanisms behind this regulation are not fully understood yet. Recent proteomic work carried out in the model legume Medicago truncatula provided the first indications of a possible involvement of nodule methionine (Met) biosynthesis and related pathways in response to water‐deficit conditions. To better understand this involvement, the drought‐induced changes in expression and content of enzymes involved in the biosynthesis of Met, S‐adenosyl‐L‐methionine (SAM) and ethylene in M. truncatula root and nodules were analyzed using targeted approaches. Nitrogen‐fixing plants were subjected to a progressive water deficit and a subsequent recovery period. Besides the physiological characterization of the plants, the content of total sulphur, sulphate and main S‐containing metabolites was measured. Results presented here show that S availability is not a limiting factor in the drought‐induced decline of nitrogen fixation rates in M. truncatula plants and provide evidences for a down‐regulation of the Met and ethylene biosynthesis pathways in roots and nodules in response to water‐deficit conditions.

ACS Style

Estíbaliz Larrainzar; Johanna A. Molenaar; Stefanie Wienkoop; Erena Gil-Quintana; Bénédicte Alibert; Anis M. Limami; Cesar Arrese-Igor; Esther M. González. Drought stress provokes the down-regulation of methionine and ethylene biosynthesis pathways inMedicago truncatularoots and nodules. Plant, Cell & Environment 2014, 37, 2051 -2063.

AMA Style

Estíbaliz Larrainzar, Johanna A. Molenaar, Stefanie Wienkoop, Erena Gil-Quintana, Bénédicte Alibert, Anis M. Limami, Cesar Arrese-Igor, Esther M. González. Drought stress provokes the down-regulation of methionine and ethylene biosynthesis pathways inMedicago truncatularoots and nodules. Plant, Cell & Environment. 2014; 37 (9):2051-2063.

Chicago/Turabian Style

Estíbaliz Larrainzar; Johanna A. Molenaar; Stefanie Wienkoop; Erena Gil-Quintana; Bénédicte Alibert; Anis M. Limami; Cesar Arrese-Igor; Esther M. González. 2014. "Drought stress provokes the down-regulation of methionine and ethylene biosynthesis pathways inMedicago truncatularoots and nodules." Plant, Cell & Environment 37, no. 9: 2051-2063.

Journal article
Published: 01 January 2014 in European Psychiatry
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IntroductionDisturbed body image perception is a common feature in eating disorders. To further investigate the etiology of these disorders, we have to study in depth body image perception during puberty in non clinical samplesObjectivesTo analyze the changes and evolution of body image perception in boys and girls from age 10 to 14.MethodsEmotional, cognitive and behavioral aspects of the body image were evaluated in order to determine the changes that take place during these years and if there were differences between genders. The sample consisted in one hundred school 10-years old boys and girls of the general population of Extremadura Community, who were followed-up for 4 years. Assessment tools were: BPSS, BSQ, DST, BIAQ and EDI. For the statistic analysis, repeated measures ANOVA was used.ResultsBetween 10 and 13 years body image perception improves in boys and worsens in girls. From age 11 and after these differences start to be statistically significant between boys and girls, the satisfaction with body image increasing in boys and remaing low in girls.ConclusionsGirls show more problems with body image perception than boys during these years. Further investigations during these period of life would be necessary.

ACS Style

M.I. Ramos; F.J. Vaz; L. Rodríguez; J.M. Cebria; N. Fernandez; E.M. González; M.I. Casado. EPA-0407 – Differences in perception of body image between boys and girls during puberty. European Psychiatry 2014, 29, 1 .

AMA Style

M.I. Ramos, F.J. Vaz, L. Rodríguez, J.M. Cebria, N. Fernandez, E.M. González, M.I. Casado. EPA-0407 – Differences in perception of body image between boys and girls during puberty. European Psychiatry. 2014; 29 ():1.

Chicago/Turabian Style

M.I. Ramos; F.J. Vaz; L. Rodríguez; J.M. Cebria; N. Fernandez; E.M. González; M.I. Casado. 2014. "EPA-0407 – Differences in perception of body image between boys and girls during puberty." European Psychiatry 29, no. : 1.

Journal article
Published: 11 April 2013 in Journal of Experimental Botany
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Drought stress is a major factor limiting symbiotic nitrogen fixation (NF) in soybean crop production. However, the regulatory mechanisms involved in this inhibition are still controversial. Soybean plants were symbiotically grown in a split-root system (SRS), which allowed for half of the root system to be irrigated at field capacity while the other half remained water deprived. NF declined in the water-deprived root system while nitrogenase activity was maintained at control values in the well-watered half. Concomitantly, amino acids and ureides accumulated in the water-deprived belowground organs regardless of transpiration rates. Ureide accumulation was found to be related to the decline in their degradation activities rather than increased biosynthesis. Finally, proteomic analysis suggests that plant carbon metabolism, protein synthesis, amino acid metabolism, and cell growth are among the processes most altered in soybean nodules under drought stress. Results presented here support the hypothesis of a local regulation of NF taking place in soybean and downplay the role of ureides in the inhibition of NF.

ACS Style

Erena Gil-Quintana; Estíbaliz Larrainzar; Amaia Seminario; Juan Luis Díaz-Leal; Josefa M. Alamillo; Manuel Pineda; Cesar Arrese-Igor; Stefanie Wienkoop; Esther M. González. Local inhibition of nitrogen fixation and nodule metabolism in drought-stressed soybean. Journal of Experimental Botany 2013, 64, 2171 -2182.

AMA Style

Erena Gil-Quintana, Estíbaliz Larrainzar, Amaia Seminario, Juan Luis Díaz-Leal, Josefa M. Alamillo, Manuel Pineda, Cesar Arrese-Igor, Stefanie Wienkoop, Esther M. González. Local inhibition of nitrogen fixation and nodule metabolism in drought-stressed soybean. Journal of Experimental Botany. 2013; 64 (8):2171-2182.

Chicago/Turabian Style

Erena Gil-Quintana; Estíbaliz Larrainzar; Amaia Seminario; Juan Luis Díaz-Leal; Josefa M. Alamillo; Manuel Pineda; Cesar Arrese-Igor; Stefanie Wienkoop; Esther M. González. 2013. "Local inhibition of nitrogen fixation and nodule metabolism in drought-stressed soybean." Journal of Experimental Botany 64, no. 8: 2171-2182.