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The demand for high-quality nutritional products has increased fruit consumption, as grapes, for this reason postharvest techniques are required to prevent losses, to preserve quality, to extend shelf life, and to attend to consumer needs. In this way, the objective of this study was to evaluate strategies to control gray mold caused by Botrytis cinerea in ‘BRS Nubia’ grapes during cold storage and shelf life periods. Grape bunches were harvested from a commercial vineyard in Marialva, Parana, Brazil. Grapes were subjected to the following treatments: cold storage at 2 ºC (control), cold storage at 2 ºC with SO2-generating pads, cold storage at 2 ºC and inoculated with B. cinerea suspension, and cold storage at 2 ºC with SO2-generating pads and inoculated with B. cinerea suspension. The experiment was conducted in a complete randomized design with five replications per treatment using four bunches per experimental unit. A factorial arrangement (absence/presence of SO2 pads × absence/presence of Botrytis inoculation) was applied. At the end of 30 days of cold storage and 7 days of shelf life (22 ºC), gray mold incidence, shattered berries, and physicochemical parameters were evaluated. The gray mold incidence on ‘BRS Nubia’ grapes decreased when SO2-generating pads were used during cold storage. Berry weight loss was greater in the treatments without SO2-generating pads after 30 days of cold storage followed by 7 days of shelf life. Berry firmness, soluble solids content (SS), total acidity (TA), SS/TA ratio, and anthocyanins concentration were not negatively affected by SO2-generating pad treatments. However, a slight increase in the shattered berries percentage was recorded for the SO2-generating pad treatments. No significant quality loss of ‘BRS Nubia’ grape was evident after 30 days of cold storage followed by 7 days of exposure at room temperature. In this context, SO2-generating pads can be used to control the gray mold incidence on ‘BRS Nubia’ table grapes during cold storage.
Ronan Carlos Colombo; UNIVERSIDADE TECNOLÓGICA FEDERAL DO PARANÁ; Deived Uilian De Carvalho; Maria Aparecida Da Cruz; Ciro Hideki Sumida; Saeed Ahmed; Muhammad Shahab; Sergio Ruffo Roberto; Universidade Estadual de Londrina; University of Swabi. Control of postharvest gray mold of ‘BRS Nubia’ table grape under cold storage. Semina: Ciências Agrárias 2020, 41, 3457 -3465.
AMA StyleRonan Carlos Colombo, UNIVERSIDADE TECNOLÓGICA FEDERAL DO PARANÁ, Deived Uilian De Carvalho, Maria Aparecida Da Cruz, Ciro Hideki Sumida, Saeed Ahmed, Muhammad Shahab, Sergio Ruffo Roberto, Universidade Estadual de Londrina, University of Swabi. Control of postharvest gray mold of ‘BRS Nubia’ table grape under cold storage. Semina: Ciências Agrárias. 2020; 41 (6supl2):3457-3465.
Chicago/Turabian StyleRonan Carlos Colombo; UNIVERSIDADE TECNOLÓGICA FEDERAL DO PARANÁ; Deived Uilian De Carvalho; Maria Aparecida Da Cruz; Ciro Hideki Sumida; Saeed Ahmed; Muhammad Shahab; Sergio Ruffo Roberto; Universidade Estadual de Londrina; University of Swabi. 2020. "Control of postharvest gray mold of ‘BRS Nubia’ table grape under cold storage." Semina: Ciências Agrárias 41, no. 6supl2: 3457-3465.
Determining the harvest date of table grapes is very important to achieve high-quality bunches with adequate soluble solids content, low titratable acidity, and high concentrations of polyphenols. Table grape consumption has increased worldwide due to its phenolic compound content and its beneficial effects on human health. Thus, this study aimed to characterize the phenolic ripening of ‘BRS Vitoria’ seedless table grapes at different ripening stages using HPLC–DAD–ESI-MS/MS. For this purpose, a trial was carried out during 2016 in a commercial vineyard of ‘BRS Vitoria’ seedless grape located in Marialva, state of Parana (Southern Brazil). Berry samples were assessed weekly, starting at véraison until full ripeness. At each ripening stage, the berries were analyzed to determine their physicochemical characteristics and polyphenolic profile. It was observed that ‘BRS Vitoria’ grapes can be harvested approximately 28 days after véraison, when the berries reach soluble solids content higher than 15°Brix and low titratable acidity. The grapes presented a typical anthocyanin profile of hybrid grapes, composed of 3-glucoside and 3,5-diglucoside derivatives. In addition, pelargonidin traces were also observed, and this aglycone is rarely detected in grapes. The total anthocyanin concentration, as malvidin-3,5-diglucoside equivalents, is close to 596.9 mg kg−1. Concerning flavonols, myricetin and quercetin are detected in greater proportions, and this cultivar can also be considered an important source of proanthocyanidins.
Ronan Carlos Colombo; Sergio Ruffo Roberto; Maria Aparecida da Cruz; Deived Uilian de Carvalho; Lilian Yukari Yamamoto; Suzana Lucy Nixdorf; José Pérez-Navarro; Sergio Gómez-Alonso; Muhammad Shahab; Saeed Ahmed; Leandro Simões Azeredo Gonçalves; Reginaldo Teodoro de Souza; Isidro Hermosín-Gutiérrez. Characterization of the phenolic ripening development of ‘BRS Vitoria’ seedless table grapes using HPLC–DAD–ESI-MS/MS. Journal of Food Composition and Analysis 2020, 95, 103693 .
AMA StyleRonan Carlos Colombo, Sergio Ruffo Roberto, Maria Aparecida da Cruz, Deived Uilian de Carvalho, Lilian Yukari Yamamoto, Suzana Lucy Nixdorf, José Pérez-Navarro, Sergio Gómez-Alonso, Muhammad Shahab, Saeed Ahmed, Leandro Simões Azeredo Gonçalves, Reginaldo Teodoro de Souza, Isidro Hermosín-Gutiérrez. Characterization of the phenolic ripening development of ‘BRS Vitoria’ seedless table grapes using HPLC–DAD–ESI-MS/MS. Journal of Food Composition and Analysis. 2020; 95 ():103693.
Chicago/Turabian StyleRonan Carlos Colombo; Sergio Ruffo Roberto; Maria Aparecida da Cruz; Deived Uilian de Carvalho; Lilian Yukari Yamamoto; Suzana Lucy Nixdorf; José Pérez-Navarro; Sergio Gómez-Alonso; Muhammad Shahab; Saeed Ahmed; Leandro Simões Azeredo Gonçalves; Reginaldo Teodoro de Souza; Isidro Hermosín-Gutiérrez. 2020. "Characterization of the phenolic ripening development of ‘BRS Vitoria’ seedless table grapes using HPLC–DAD–ESI-MS/MS." Journal of Food Composition and Analysis 95, no. : 103693.
The development and evaluation of new cultivars of seedless grapes (Vitis spp.) with good yield and adapted to different edaphoclimatic conditions are essential to increase the competitiveness of the productive system. The present work had the objective to characterize the phenology and the yield of the new hybrid seedless grape ‘BRS Melodia’, grown in an annual double cropping system in a subtropical region. The evaluations were carried out during the 2013 summer cropping period and the 2014 off-season cropping period in a commercial area located in Marialva, PR, Brazil. The ‘BRS Melodia’ scions were grafted onto ‘IAC 766 Campinas’ rootstocks, trained in an overhead trellis system spaced at 2.5 × 2.5 m. In both seasons, the duration in days of the main phenological phases of the vines, as well as their thermal demand and their physicochemical and productive characteristics were evaluated. The ‘BRS Melodia’ seedless grapevines presented a cycle of 138 days and yield of 23.85 tons.ha−1 in the summer season, and 121 days and yield of 19.4 tons.ha−1 in the off-season crop. The soluble solids, titratable acidity, and maturation index were 15.1 °Brix, 0.5% tartaric acid and 28.5, respectively, in the summer season and 15.4 °Brix, 0.6% tartaric acid and 25.6 in the off-season crop, indicating a possibility of cultivate this new hybrid seedless grape under an annual double cropping system in subtropical conditions.
Renata Koyama; Wellington Fernando Silva Borges; Ronan Carlos Colombo; Ibrar Hussain; Reginaldo Teodoro De Souza; Sergio Ruffo Roberto. Phenology and Yield of the Hybrid Seedless Grape ‘BRS Melodia’ Grown in an Annual Double Cropping System in a Subtropical Area. Horticulturae 2020, 6, 3 .
AMA StyleRenata Koyama, Wellington Fernando Silva Borges, Ronan Carlos Colombo, Ibrar Hussain, Reginaldo Teodoro De Souza, Sergio Ruffo Roberto. Phenology and Yield of the Hybrid Seedless Grape ‘BRS Melodia’ Grown in an Annual Double Cropping System in a Subtropical Area. Horticulturae. 2020; 6 (1):3.
Chicago/Turabian StyleRenata Koyama; Wellington Fernando Silva Borges; Ronan Carlos Colombo; Ibrar Hussain; Reginaldo Teodoro De Souza; Sergio Ruffo Roberto. 2020. "Phenology and Yield of the Hybrid Seedless Grape ‘BRS Melodia’ Grown in an Annual Double Cropping System in a Subtropical Area." Horticulturae 6, no. 1: 3.
The objective of this study was to determine the ideal moisture degree for the cryopreservation of Cattleya labiata Lindl. and Miltonia regnellii Rchb.f. orchid seeds. Orchid seeds were standardized in relation to the desired moisture degrees (4, 6, 8, 10, 12, and 15%) and were subjected to rapid freezing in liquid nitrogen (-196 °C) for 24 h; then, the seeds were defrosted and analyzed. For both orchid species, the seeds that were immersed in liquid nitrogen with 4% moisture content had the highest viability percentage, averaging 95% and 68% of viable seeds for C. labiata and M. regnellii, respectively. The seeds with 12% and 15% moisture content had no viable seeds. For the fresh mass of seedlings, there was no significant difference between moisture content treatments for either species.
Edilene Aparecida Preti Ferrari; Ronan Carlos Colombo; Ricardo Tadeu De Faria; Carmen Silvia Vieira Janeiro Neves; Franciele Da Silva Vero. Degree of moisture in seeds for the cryopreservation of orchids native to Brazil. Ciência Rural 2020, 50, 1 .
AMA StyleEdilene Aparecida Preti Ferrari, Ronan Carlos Colombo, Ricardo Tadeu De Faria, Carmen Silvia Vieira Janeiro Neves, Franciele Da Silva Vero. Degree of moisture in seeds for the cryopreservation of orchids native to Brazil. Ciência Rural. 2020; 50 (8):1.
Chicago/Turabian StyleEdilene Aparecida Preti Ferrari; Ronan Carlos Colombo; Ricardo Tadeu De Faria; Carmen Silvia Vieira Janeiro Neves; Franciele Da Silva Vero. 2020. "Degree of moisture in seeds for the cryopreservation of orchids native to Brazil." Ciência Rural 50, no. 8: 1.
Grapes grown under warm climate conditions tend to show less color development, and this phenomenon is attributed to low anthocyanin accumulation in berry skin in response to high temperature during ripening. ‘Benitaka’ (Vitis vinifera L.) is a table grape that shows poor color development when grown under a subtropical condition, and the exogenous application of (S)-cis-abscisic acid (S-ABA) is a recently developed technique used to overcome this difficulty. Trials were conducted during two crop seasons in a commercial vineyard containing 11-year-old ‘Benitaka’ vines grafted onto ‘IAC 766 Campinas’ rootstocks in Marialva, State of Parana (PR), Brazil. The vines were trained on an overhead trellis system, covered with a black plastic mash, and spaced 3.0 × 6.0 m apart. A randomized block design was used and treatments included the application of 400 mg L−1 of S-ABA at different stages of ripening, as follows: Control (no application); At pre-veraison (PRV) (7 days before veraison); At veraison (V); and At post-veraison (POV) (7 days after V). A second application was performed for all treatments, 10 days after the first application, except for the control. The analysis of berries included, total and daily anthocyanins accumulation, the color index of red grapes (CIRG), daily CIRG development, lightness (L*), chroma (C*), hue angle (h°), mass, length, width, firmness, total soluble solids (TSS), titratable acidy (TA), the index of maturation (TSS/TA), and total polyphenols of berries. The exogenous application of S-ABA from PRV to POV significantly enhanced the contents and rates of total anthocyanins accumulation and the CIRG of ‘Benitaka’ table grape; however, the application at PRV and V provided a higher response. A stronger correlation exists between anthocyanin concentration and the color index of berry skin, however, the main physicochemical characteristics of berries are not significantly affected by the use of S-ABA. Berry firmness varies in response to S-ABA application, but not to an extent at which it compromises berry quality for commercial use.
Muhammad Shahab; Sergio Ruffo Roberto; Saeed Ahmed; Ronan Carlos Colombo; João Pedro Silvestre; Renata Koyama; Reginaldo Teodoro de Souza. Relationship between anthocyanins and skin color of table grapes treated with abscisic acid at different stages of berry ripening. Scientia Horticulturae 2019, 259, 108859 .
AMA StyleMuhammad Shahab, Sergio Ruffo Roberto, Saeed Ahmed, Ronan Carlos Colombo, João Pedro Silvestre, Renata Koyama, Reginaldo Teodoro de Souza. Relationship between anthocyanins and skin color of table grapes treated with abscisic acid at different stages of berry ripening. Scientia Horticulturae. 2019; 259 ():108859.
Chicago/Turabian StyleMuhammad Shahab; Sergio Ruffo Roberto; Saeed Ahmed; Ronan Carlos Colombo; João Pedro Silvestre; Renata Koyama; Reginaldo Teodoro de Souza. 2019. "Relationship between anthocyanins and skin color of table grapes treated with abscisic acid at different stages of berry ripening." Scientia Horticulturae 259, no. : 108859.
Passion fruits species presents a larger economic importance for in natura consumption and industry. The main way to propagate these species is by seeds; however, the aril presence and environmental conditions may inhibit the seeds germination. In this sense, the objective of this study was to evaluate the effect of aril removal methods and light conditions on P. edulis var. flavicarpa seeds quality and germination. There were used five methods to remove the seeds aril: (1) friction on steel mesh sieve for 5 minutes; (2) friction with coarse sand on steel mesh sieve for 5 minutes; (3) immersion in lime solution at 10% (w/v), for 30 minutes; (4) fermentation in sugar solution at 10% (w/v), during 48 hours; and (5) natural pulp fermentation, during five days. The seeds were submitted to germination test at darkness and lightness, as well as, to length seedlings and emergence test. Regardless the aril removal method, the germination percentage was lower at lightness than darkness highlighting the natural pulp fermentation treatment, which presented 86% of seeds germinated. On the other hand, the aril removal methods did not affect the seedlings emergence, with emergence percentages ranged from 88 to 94%.
R. C. Colombo; Denis Costa; D. U. Carvalho; M. A. Cruz; S. R. Roberto. Methods of aril removal and lightness conditions on seeds physiological quality of sour passion fruits. Brazilian Journal of Biology 2019, 79, 404 -409.
AMA StyleR. C. Colombo, Denis Costa, D. U. Carvalho, M. A. Cruz, S. R. Roberto. Methods of aril removal and lightness conditions on seeds physiological quality of sour passion fruits. Brazilian Journal of Biology. 2019; 79 (3):404-409.
Chicago/Turabian StyleR. C. Colombo; Denis Costa; D. U. Carvalho; M. A. Cruz; S. R. Roberto. 2019. "Methods of aril removal and lightness conditions on seeds physiological quality of sour passion fruits." Brazilian Journal of Biology 79, no. 3: 404-409.
A well-developed root system is a very important characteristic of rootstocks. Initial plant characterization is a potential technique to highlight cultivars with desirable root architecture for the diversification of rootstocks in the national citriculture. Thus, the aim of this study was to evaluate the initial shoot development and root architecture of citrus cultivars used as rootstocks. Seeds from five rootstocks, ‘Cleopatra’ mandarin, ‘Rangpur’ lime, ‘Rough’ lemon, ‘C13’ and ‘Troyer’ citranges, were sown in black polyethylene bags. A completely randomized block design with five replications and one plant per plot was applied. At 30 and 45 days after emergence, shoots and roots were collected for analysis. Shoot analysis measured the total number of leaves, leaf area, stem diameter, plant height, and fresh and dry matter weights. Root analysis evaluated fresh and dry matter weights and root architecture by image analysis, which recorded root traits including primary, secondary, and tertiary root numbers; length and total volume; and the percentage of secondary roots present in the upper third of the primary root. Shoot and root data of fresh and dry weights were submitted to variance analysis and compared using Tukey’s test (5%). The remaining root data were standardized for variance 1 and studied through principal component analysis. The ‘C13’ citrange showed good shoot development, with greater leaf area, plant height, stem diameter, and shoot fresh and dry matter weights during both evaluation periods, followed by the ‘Troyer’ citrange. The ‘Cleopatra’ mandarin had the smallest shoot and root development during both evaluation periods, presenting lower primary root, secondary root, and root volume than other cultivars evaluated at the same time. The ‘Troyer’ citrange had fewer roots in the upper third of the primary root and a higher root insertion angle.
Maria Aparecida Da Cruz; Deived Uilian De Carvalho; Denis Santiago Da Costa; Ronan Carlos Colombo; Camilla De Andrade Pacheco; Zuleide Hissano Tazima; Carmen Silvia Vieira Janeiro Neves. Initial shoot development and root architecture of citrus rootstocks. Semina: Ciências Agrárias 2019, 40, 1393 -1404.
AMA StyleMaria Aparecida Da Cruz, Deived Uilian De Carvalho, Denis Santiago Da Costa, Ronan Carlos Colombo, Camilla De Andrade Pacheco, Zuleide Hissano Tazima, Carmen Silvia Vieira Janeiro Neves. Initial shoot development and root architecture of citrus rootstocks. Semina: Ciências Agrárias. 2019; 40 (4):1393-1404.
Chicago/Turabian StyleMaria Aparecida Da Cruz; Deived Uilian De Carvalho; Denis Santiago Da Costa; Ronan Carlos Colombo; Camilla De Andrade Pacheco; Zuleide Hissano Tazima; Carmen Silvia Vieira Janeiro Neves. 2019. "Initial shoot development and root architecture of citrus rootstocks." Semina: Ciências Agrárias 40, no. 4: 1393-1404.
In colored table grapes, the anthocyanin contents are inhibited by the high temperature during ripening and berries suffer a lack of skin color, thus affecting their market value. In order to overcome this issue, a research study was planned to evaluate the influence of (S)-cis-abscisic acid (S-ABA) on rates of anthocyanin accumulation in table grapes when applied at different timings of ripening, and to quantify the gradual increase of berry color. The study was conducted in a commercial vineyard of ‘Benitaka’ table grapes (Vitis vinifera L.), grown under double annual cropping system in a subtropical area. The trials were carried out during two consecutive seasons (i.e., summer season of 2015 and off-season of 2016). The treatments used for the experiments contained 400 mg L−1 S-ABA applied at different timings of veraison (the onset of ripening), as follows: control (with no application); at pre-veraison (PRV); at veraison (V); and at post-veraison (POV). For all S-ABA treatments, a second application was performed 10 days after the first application. Berries were analyzed for weekly and daily anthocyanin accumulations, weekly and daily color index development (CIRG), total soluble solids (TSS) content, titratable acidity (TA), and maturation index (TSS/TA). Grapes subjected to exogenous application of S-ABA at any time of veraison, especially at PRV or at V, significantly increased the anthocyanin accumulation as well as berry color index development. Other chemical properties of grapes (i.e., TSS, TA, and TSS/TA evolution) were not affected by the use of S-ABA and followed a predictable pattern in relation to days of berries ripening.
Muhammad Shahab; Sergio Ruffo Roberto; Saeed Ahmed; Ronan Carlos Colombo; João Pedro Silvestre; Renata Koyama; Reginaldo Teodoro De Souza. Anthocyanin Accumulation and Color Development of ‘Benitaka’ Table Grape Subjected to Exogenous Abscisic Acid Application at Different Timings of Ripening. Agronomy 2019, 9, 164 .
AMA StyleMuhammad Shahab, Sergio Ruffo Roberto, Saeed Ahmed, Ronan Carlos Colombo, João Pedro Silvestre, Renata Koyama, Reginaldo Teodoro De Souza. Anthocyanin Accumulation and Color Development of ‘Benitaka’ Table Grape Subjected to Exogenous Abscisic Acid Application at Different Timings of Ripening. Agronomy. 2019; 9 (4):164.
Chicago/Turabian StyleMuhammad Shahab; Sergio Ruffo Roberto; Saeed Ahmed; Ronan Carlos Colombo; João Pedro Silvestre; Renata Koyama; Reginaldo Teodoro De Souza. 2019. "Anthocyanin Accumulation and Color Development of ‘Benitaka’ Table Grape Subjected to Exogenous Abscisic Acid Application at Different Timings of Ripening." Agronomy 9, no. 4: 164.
This study aimed to determine NO3 - /NH4 + proportion delivered by fertigation that allows greater desert rose plants growth in pots. Plants were cultivated in greenhouse in polypropylene pots, using a mixture of sand and composted Pinus powder (1:1, v v-1) as substrate. Experimental design was completely randomized, with six treatments (control, 0/100 25/75, 50/50, 75/25 and 100/0 of NO3 – and NH4 + respectively), and ten replications. Fertigation was performed weekly in the amount of 100 mL per pot for 180 days. Variables evaluated were: shoot height, basal diameter of the caudice, number of branches, roots, leaves and caudice dry mass and macronutrient contents determination and accumulation in leaves, caudice and root. NO3 – and NH4 + proportion promoted a greater change in nutrient content in leaves compared to caudice and roots. The proportion 25/75 NO3 – /NH4 + is recommended for desert rose cultivation, as it provides the best results for most of the characteristics studied.
Vanessa Stegani; Guilherme Augusto Cito Alves; Thadeu De Melo Rodrigues; Ronan Carlos Colombo; Guilherme Biz; Ricardo Tadeu De Faria. Crescimento de rosa do deserto fertirrigada com diferentes proporções de nitrato/amônio. Ornamental Horticulture 2019, 25, 18 -25.
AMA StyleVanessa Stegani, Guilherme Augusto Cito Alves, Thadeu De Melo Rodrigues, Ronan Carlos Colombo, Guilherme Biz, Ricardo Tadeu De Faria. Crescimento de rosa do deserto fertirrigada com diferentes proporções de nitrato/amônio. Ornamental Horticulture. 2019; 25 (1):18-25.
Chicago/Turabian StyleVanessa Stegani; Guilherme Augusto Cito Alves; Thadeu De Melo Rodrigues; Ronan Carlos Colombo; Guilherme Biz; Ricardo Tadeu De Faria. 2019. "Crescimento de rosa do deserto fertirrigada com diferentes proporções de nitrato/amônio." Ornamental Horticulture 25, no. 1: 18-25.
Studies on rootstocks and scions are of major importance for citrus crop production. The goal of this study was to evaluate vegetative growth, yield performance, and fruit quality of ‘Navelina’ sweet orange trees grafted on five different rootstocks in Northern Parana state, Brazil. The experimental design was completely randomized blocks, with six replications, two plants per plot, and five treatments: ‘Rangpur’ lime; ‘Cleopatra’ mandarin, ‘Sunki’ mandarin; ‘Swingle’ citrumelo; and ‘Fepagro C-13’ citrange. Tree vegetative growth, yield performance, and fruit physical-chemical characteristics were analyzed. ‘Sunki’ mandarin provided the largest canopy volume for ‘Navelina’ sweet orange trees, whereas ‘Swingle’ citrumelo, ‘Fepagro C-13’ citrange, and ‘Rangpur’ lime provided a smaller volume for tree canopy. Compared to Rangpur lime, ‘Swingle’ citrumelo provided higher accumulated yield and production efficiency for ‘Navelina’ sweet orange trees. All rootstocks induced good physical and chemical quality to ‘Navelina’ sweet orange fruits.
Maria Aparecida Da Cruz; Carmen Silvia Vieira Janeiro Neves; Deived Uilian De Carvalho; Ronan Carlos Colombo; Rui Pereira Leite Júnior; Zuleide Hissano Tazima. ‘Navelina’ sweet orange trees on five rootstocks in Northern Parana state, Brazil. Revista Brasileira de Fruticultura 2019, 41, 1 .
AMA StyleMaria Aparecida Da Cruz, Carmen Silvia Vieira Janeiro Neves, Deived Uilian De Carvalho, Ronan Carlos Colombo, Rui Pereira Leite Júnior, Zuleide Hissano Tazima. ‘Navelina’ sweet orange trees on five rootstocks in Northern Parana state, Brazil. Revista Brasileira de Fruticultura. 2019; 41 (3):1.
Chicago/Turabian StyleMaria Aparecida Da Cruz; Carmen Silvia Vieira Janeiro Neves; Deived Uilian De Carvalho; Ronan Carlos Colombo; Rui Pereira Leite Júnior; Zuleide Hissano Tazima. 2019. "‘Navelina’ sweet orange trees on five rootstocks in Northern Parana state, Brazil." Revista Brasileira de Fruticultura 41, no. 3: 1.
The aim of this study was to evaluate the performance of plant regulators on seed germination of different atemoya cultivars: Thompson, Gefner and PR-1. The seeds were extracted from ripe fruits and submitted to the following treatments: 50 mg L-1 gibberellic acid (GA3), 10 ml kg-1 of seeds (Stimulate®), 6 ml kg-1 of seeds (Evolust®), and control. The experimental design was completely randomized in a factorial 4x3 (three seed treatments + control x three cultivars), using five samples of 25 seeds. The seeds were sowed on paper and placed in a germination chamber, using alternated temperature 20-30oC (16h - 8h) under light absence. There were evaluated the percentage of germinated seeds, normal seedlings, abnormal seedlings, and the germination speed index. The Thompson cultivar presented the highest percentage of germinated seeds, normal seedlings, and germination speed index, followed by the Gefner cultivar. Among the products, the GA3 promoted greater germination percentage. Using GA3 and Stimulate®, as a pre-germination treatment, provided high percentage of normal seedlings. The products utilized did not affect the germination speed index.
Deived Uilian De Carvalho; Maria Aparecida Da Cruz; Elisete Aparecida Fernandes Osipi; Conceição Aparecida Cossa; Ronan Carlos Colombo; Maria Aparecida Fonseca Sorace. PLANT GROWTH REGULATORS ON ATEMOYA SEEDS GERMINATION. Nucleus 2018, 15, 457 -462.
AMA StyleDeived Uilian De Carvalho, Maria Aparecida Da Cruz, Elisete Aparecida Fernandes Osipi, Conceição Aparecida Cossa, Ronan Carlos Colombo, Maria Aparecida Fonseca Sorace. PLANT GROWTH REGULATORS ON ATEMOYA SEEDS GERMINATION. Nucleus. 2018; 15 (2):457-462.
Chicago/Turabian StyleDeived Uilian De Carvalho; Maria Aparecida Da Cruz; Elisete Aparecida Fernandes Osipi; Conceição Aparecida Cossa; Ronan Carlos Colombo; Maria Aparecida Fonseca Sorace. 2018. "PLANT GROWTH REGULATORS ON ATEMOYA SEEDS GERMINATION." Nucleus 15, no. 2: 457-462.
Adenium obesum, also known as desert rose, has recently become popular in Brazil, due to its exuberance and sculptural shape. However, the production of the species in commercial scale has been facing challenges regarding propagation of hybrids by in vitro cultivation techniques, potting medium composition, irrigation and fertilization management, disease and pest control. Unlike other species sold as potted plants, there are no commercial production protocols established for Adenium, mainly due to the scarcity of technical information about this species. Thus, this technical article aimed to provide information related to the Adenium growth, in view of its commercial scale production. In addition, aspects related to floral morphology, pollination and seed production of the species, as well as, vegetative propagation strategies were discussed.
Ronan Carlos Colombo; Maria Aparecida Da Cruz; Deived Uilian Carvalho; Rodrigo Thibes Hoshino; Guilherme Augusto Cito Alves; Ricardo Tadeu De Faria. Adenium obesum as a new potted flower: growth management. Ornamental Horticulture 2018, 24, 197 -205.
AMA StyleRonan Carlos Colombo, Maria Aparecida Da Cruz, Deived Uilian Carvalho, Rodrigo Thibes Hoshino, Guilherme Augusto Cito Alves, Ricardo Tadeu De Faria. Adenium obesum as a new potted flower: growth management. Ornamental Horticulture. 2018; 24 (3):197-205.
Chicago/Turabian StyleRonan Carlos Colombo; Maria Aparecida Da Cruz; Deived Uilian Carvalho; Rodrigo Thibes Hoshino; Guilherme Augusto Cito Alves; Ricardo Tadeu De Faria. 2018. "Adenium obesum as a new potted flower: growth management." Ornamental Horticulture 24, no. 3: 197-205.
‘BRS Isis’ is a novel hybrid seedless table grape with large reddish bunches. This cultivar is cultivated in tropical and subtropical areas and has potential to be exported overseas; however, under these growth conditions, grapes can be severely damaged by gray mold (Botrytis cinerea), the main postharvest disease of table grapes. Thus, this study aimed to evaluate different pre- and postharvest strategies to extend the storage period of ‘BRS Isis’ seedless table grapes. The treatments consisted of grapes packed into carton boxes under cold storage (1 °C and high relative humidity), pre- and/or postharvest treatment with potassium bicarbonate or a biological control agent (Bacillus subtilis), all with Botrytis inoculation. Additionally, two controls, with and without Botrytis inoculation, were also evaluated. The experimental design was completely randomized with eight treatments and three replications, and each plot consisted of four bunches. After a 50-day cold storage period, the carton boxes were kept for five days at room temperature (22 °C). Gray mold incidence and water loss, as well as soluble solids (SS), pH, titratable acidity (TA), SS/TA ratio, and color attributes were evaluated during both periods. Cold storage effectively reduced gray mold in ‘BRS Isis’ seedless grape for a period of 50 days, even when grapes were inoculated; however, no difference among treatments was observed. Higher water loss was observed in both non-treated control treatments.
Ronan Carlos Colombo; Deived Uilian Carvalho; Maria Aparecida Da Cruz; Ciro Hideki Sumida; Saeed Ahmed; Paulo Augusto Bassoli; Reginaldo Teodoro De Souza; Sergio Ruffo Roberto. Cold Storage and Biocontrol Agents to Extend the Storage Period of ‘BRS Isis’ Seedless Table Grapes. Horticulturae 2018, 4, 18 .
AMA StyleRonan Carlos Colombo, Deived Uilian Carvalho, Maria Aparecida Da Cruz, Ciro Hideki Sumida, Saeed Ahmed, Paulo Augusto Bassoli, Reginaldo Teodoro De Souza, Sergio Ruffo Roberto. Cold Storage and Biocontrol Agents to Extend the Storage Period of ‘BRS Isis’ Seedless Table Grapes. Horticulturae. 2018; 4 (3):18.
Chicago/Turabian StyleRonan Carlos Colombo; Deived Uilian Carvalho; Maria Aparecida Da Cruz; Ciro Hideki Sumida; Saeed Ahmed; Paulo Augusto Bassoli; Reginaldo Teodoro De Souza; Sergio Ruffo Roberto. 2018. "Cold Storage and Biocontrol Agents to Extend the Storage Period of ‘BRS Isis’ Seedless Table Grapes." Horticulturae 4, no. 3: 18.
The nursery plants supply is one of the most important factors that limites blueberries production in some areas, due to some propagation difficulties observed on this species. In this way, this study aimed to evaluate substrates and IBA application methods on ‘Powderblue’ blueberries (Vaccinium ashei) minicuttings rooting, as well as to verify the rooting development in different periods. The study was divided in two experiments. The first one aimed to evaluate ‘Powderblue’ blueberry minicuttings rooting in three substrates (pine bark, carbonized rice husk and vermiculite) in response to two indolebutyric acid (IBA) application methods: 50% alcohol solution and talc. The second experiment aimed to evaluate ‘Powderblue’ blueberry minicuttings rooting using the best substrate recorded in the first trial (pine bark) and two IBA application methods associated to three periods of rooting access, in order to obtain the ideal rooting period and root development. In the first experiment the rooting percentage presented significant difference among the substrates; the higher percentage of rooted minicuttings occurred in pine bark substrate (68%). Regarding the methods used to IBA application, the talc method was more efficient to promote minicuttings rooting. In the second experiment, it was observed an increase in cutting rooting percentage from 55 to 84%, according to the evaluated seasons; however, the IBA application method did not influence the rooting percentage. Therefore, to produce ‘Powderblue’ blueberry plants from minicuttings it is recommended to use pine bark as substrate and talc as method to IBA application, transplanting the nurseries at 110 days after planting.
Ronan C. Colombo; Deived U. De Carvalho; Maria A. Da Cruz; Sergio R. Roberto. Blueberry Propagation by Minicuttings in Response to Substrates and Indolebutyric Acid Application Methods. Journal of Agricultural Science 2018, 10, p450 .
AMA StyleRonan C. Colombo, Deived U. De Carvalho, Maria A. Da Cruz, Sergio R. Roberto. Blueberry Propagation by Minicuttings in Response to Substrates and Indolebutyric Acid Application Methods. Journal of Agricultural Science. 2018; 10 (9):p450.
Chicago/Turabian StyleRonan C. Colombo; Deived U. De Carvalho; Maria A. Da Cruz; Sergio R. Roberto. 2018. "Blueberry Propagation by Minicuttings in Response to Substrates and Indolebutyric Acid Application Methods." Journal of Agricultural Science 10, no. 9: p450.
The objective of this work was to evaluate cold storage periods and postharvest longevity of the seedless table grape 'BRS Vitória' subjected to the application of the disease-resistance inducer acibenzolar-S-methyl (ASM). Bunches were treated or not with 1% ASM, placed individually in plastic clamshell trays and stored in a cold chamber at 2±1ºC, at a high relative humidity, for three periods (30, 45, and 60 days), followed by a 5-day period of shelf life in plastic clamshells, at 22±2ºC. After each storage period, gray mold (Botrytis cinerea) incidence was evaluated, and the physicochemical analysis was performed. A completely randomized design, with four replicates, was used in two 2x3 factorial arrangements: one evaluating ASM application and cold storage periods; and another, ASM and cold storage + shelf-life periods. ASM did not change the physicochemical characteristics of the bunches, and the incidence of gray mold was lower than 1% in all treatments. Water loss from bunches increased with storage periods. Even without ASM application, 'BRS Vitória' grape can preserve its postharvest quality for up to 60 days of cold storage, plus five days of shelf life in plastic clamshells.
Ronan Carlos Colombo; Reginaldo Teodoro De Souza; Maria Aparecida Da Cruz; Deived Uilian De Carvalho; Renata Koyama; Ana Paula Bilck; Sergio Ruffo Roberto. Postharvest longevity of 'BRS Vitória' seedless grapes subjected to cold storage and acibenzolar-S-methyl application. Pesquisa Agropecuária Brasileira 2018, 53, 809 -814.
AMA StyleRonan Carlos Colombo, Reginaldo Teodoro De Souza, Maria Aparecida Da Cruz, Deived Uilian De Carvalho, Renata Koyama, Ana Paula Bilck, Sergio Ruffo Roberto. Postharvest longevity of 'BRS Vitória' seedless grapes subjected to cold storage and acibenzolar-S-methyl application. Pesquisa Agropecuária Brasileira. 2018; 53 (7):809-814.
Chicago/Turabian StyleRonan Carlos Colombo; Reginaldo Teodoro De Souza; Maria Aparecida Da Cruz; Deived Uilian De Carvalho; Renata Koyama; Ana Paula Bilck; Sergio Ruffo Roberto. 2018. "Postharvest longevity of 'BRS Vitória' seedless grapes subjected to cold storage and acibenzolar-S-methyl application." Pesquisa Agropecuária Brasileira 53, no. 7: 809-814.
The pH exudate is a quick test used to check the seed membranes integrity; however, its performance is limited due to the singularity of each specie requiring adjustments about its methodology. In this sense, the aim of this study was to perform a quick and accurate physiological potential estimation of Citrus limonia Osb. (‘Rangpur Lime’) seeds through pH exudate test. First, there was carried out the characterization of five seedlots of ‘Rangpur Lime’ by determination of the thousand seed mass, water content, germination and emergence. Then, a complete randomized experimental design was installed in a factorial scheme: 5 × 3 × 2 (five seedlots, three indicator solutions, and two immersion periods), to evaluate the pH exudate. The seeds were individually distributed in polypropylene trays, and after the immersion periods, there were taken pictures and analyzed by a numeric scale. Despite the similarity, the emergence test showed to be more efficient for ‘Rangpur Lime’ seeds comparing to the germination test, because it promoted higher percentage of seedlings. Regarding the pH exudate test, there were not observed differences between the seedlots for both analysis, visual and image, not being efficient to discriminate lots of ‘Rangpur Lime’.
Deived Uilian De Carvalho; Denis Costa; Maria Aparecida Da Cruz; Jéssica De Lucena Marinho; Ronan Carlos Colombo; Carmen Silvia Vieira Janeiro Neves. pH exudate test as a method to estimate viability and vigor of Citrus limonia Osbeck seeds. Journal of Seed Science 2018, 40, 156 -163.
AMA StyleDeived Uilian De Carvalho, Denis Costa, Maria Aparecida Da Cruz, Jéssica De Lucena Marinho, Ronan Carlos Colombo, Carmen Silvia Vieira Janeiro Neves. pH exudate test as a method to estimate viability and vigor of Citrus limonia Osbeck seeds. Journal of Seed Science. 2018; 40 (2):156-163.
Chicago/Turabian StyleDeived Uilian De Carvalho; Denis Costa; Maria Aparecida Da Cruz; Jéssica De Lucena Marinho; Ronan Carlos Colombo; Carmen Silvia Vieira Janeiro Neves. 2018. "pH exudate test as a method to estimate viability and vigor of Citrus limonia Osbeck seeds." Journal of Seed Science 40, no. 2: 156-163.
The hybrid, obtained by back crossing between (Cattleya labiata x Cattleya forbesii) x Cattleya labiata is a vigorous plant, bi- or unifoliate, features slender and cylindrical pseudobulbs and leathery dark-greenish leaves, with oblanceolate shape format of blunt tips with the first flowering four years after sowing. In Londrina, flowering occurred twice a year, between the months of April and May and October and November with 2-4 flowers per pseudobulb and durability ranging from 15 to 20 days. The flowers of the new hybrid were purple with a labellum with a yellow center and purple stripes.
Guilherme Augusto Cito Alves; Rodrigo Thibes Hoshino; Douglas Junior Bertoncelli; Ronan Carlos Colombo; Vanessa Stegani; Ricardo Tadeu De Faria. Novo híbrido de orquídea Cattleya. Ornamental Horticulture 2018, 24, 145 -148.
AMA StyleGuilherme Augusto Cito Alves, Rodrigo Thibes Hoshino, Douglas Junior Bertoncelli, Ronan Carlos Colombo, Vanessa Stegani, Ricardo Tadeu De Faria. Novo híbrido de orquídea Cattleya. Ornamental Horticulture. 2018; 24 (2):145-148.
Chicago/Turabian StyleGuilherme Augusto Cito Alves; Rodrigo Thibes Hoshino; Douglas Junior Bertoncelli; Ronan Carlos Colombo; Vanessa Stegani; Ricardo Tadeu De Faria. 2018. "Novo híbrido de orquídea Cattleya." Ornamental Horticulture 24, no. 2: 145-148.
The desert rose (Adenium obesum) has been highlighted in the internal market, however there are not many studies related to its demands regarding its nutrition and substrates. The aim of this study was to evaluate the effect of different fertilizations and its interactions with different substrates in the initial growth of the desert rose. The seedlings of desert rose with 60 days have been potted transplanted with a capacity of 0.415 L, filled with two kinds of substrates: sand + coconut fiber and sand + composted pine powder. Four different fertilization managements have been done: without fertilization, application of the Hoagland & Arnon nutrient solution; application of NH3 NO4 at 590 g L-1 of nitrogen (N); and the application of the Hoagland & Arnon nutrient solution modified through the addition of NH3 NO4 , increasing the concentration of N to 800 g L-1. The solutions have been applied biweekly adding 50 mL per pot. The experiment has been entirely randomized in a 2x4 factorial design, with 10 repetitions. After 150 days from the beginning of the fertilizations the phytometrical parameters evaluated were: height of the shoot; diameter of the basal caudex; number of shoots; dry mass of the roots, caudex and leaves; and determination of the levels and accumulation of macronutrients in the shoot, and the pH and electrical conductivity of the substrates. The evaluated data have been submitted to an analysis of variance and the Tukey test compared through the test at 5% of error probability. For all the evaluated parameters, when the composted pine mixture was used, the seedlings showed a higher development compared to coconut fiber mixture. The seedlings had a better performance with the modified Hoagland solution for height, number of shoots, caudex diameter and leaf dry mass in both substrates. The use of the sand + pine powder (1:1 v v-1) and the fertilization with Hoagland & Arnon solution combined with the NH3 NO4 solution provided a better initial growth on the desert rose.
Guilherme Augusto Cito Alves; Rodrigo Thibes Hoshino; Douglas Junior Bertoncelli; Ana Beatryz Prenzier Suzuki; Ronan Carlos Colombo; Ricardo Tadeu De Faria. Substrates and fertilizations in the initial growth of the desert rose. Ornamental Horticulture 2018, 24, 19 -27.
AMA StyleGuilherme Augusto Cito Alves, Rodrigo Thibes Hoshino, Douglas Junior Bertoncelli, Ana Beatryz Prenzier Suzuki, Ronan Carlos Colombo, Ricardo Tadeu De Faria. Substrates and fertilizations in the initial growth of the desert rose. Ornamental Horticulture. 2018; 24 (1):19-27.
Chicago/Turabian StyleGuilherme Augusto Cito Alves; Rodrigo Thibes Hoshino; Douglas Junior Bertoncelli; Ana Beatryz Prenzier Suzuki; Ronan Carlos Colombo; Ricardo Tadeu De Faria. 2018. "Substrates and fertilizations in the initial growth of the desert rose." Ornamental Horticulture 24, no. 1: 19-27.
In the past decades, desert rose has become a very popular ornamental plant, especially among collectors, due to its exotic and sculptural forms. However, it has been grown on a commercial scale only recently, and little is known about how to best manage it as a container-grown plant, or even which potting medium (substrate) to recommend. Therefore, the aim of this study was to investigate the interactions between potting media and irrigation levels for growing desert rose as a potted ornamental plant. The experiment was conducted in a greenhouse using a 6 x 2 factorial arrangement with six replications, six potting media and two irrigation levels. The mixes were characterized by measuring their physical properties, specifically the density and water retention capacity (WRC), as well as chemical properties, such as the pH and electrical conductivity (EC). After 210 days, plant growth and plant water consumption were evaluated and measured. A lower dry density for the vermiculite mixes was observed in comparison to that for the sand mixes. However, WRC ranged from 428 to 528 mL L-1 among the mixes, values considered close to ideal. In general, plant growth exhibited higher increases in mixes consisting of coconut fiber + sand or vermiculite, regardless of the irrigation level. Mixes of vermiculite + coconut fiber and sand + coconut fiber can be used to grow desert rose in pots, as long as irrigation is used to maintain the moisture content of the potting medium (mix) between 60-70% and 80-90% of the WRC.
Ronan Carlos Colombo; Vanessa Favetta; Marcelo Augusto Aguiar E Silva; Ricardo Tadeu De Faria. Substrates and irrigation levels for growing desert rose in pots. Ciência e Agrotecnologia 2018, 42, 69 -79.
AMA StyleRonan Carlos Colombo, Vanessa Favetta, Marcelo Augusto Aguiar E Silva, Ricardo Tadeu De Faria. Substrates and irrigation levels for growing desert rose in pots. Ciência e Agrotecnologia. 2018; 42 (1):69-79.
Chicago/Turabian StyleRonan Carlos Colombo; Vanessa Favetta; Marcelo Augusto Aguiar E Silva; Ricardo Tadeu De Faria. 2018. "Substrates and irrigation levels for growing desert rose in pots." Ciência e Agrotecnologia 42, no. 1: 69-79.
Jessica Tosin Milanez; Leandro Camargo Neves; Ronan Carlos Colombo; Muhammad Shahab; Sergio Ruffo Roberto. Bioactive compounds and antioxidant activity of buriti fruits, during the postharvest, harvested at different ripening stages. Scientia Horticulturae 2018, 227, 10 -21.
AMA StyleJessica Tosin Milanez, Leandro Camargo Neves, Ronan Carlos Colombo, Muhammad Shahab, Sergio Ruffo Roberto. Bioactive compounds and antioxidant activity of buriti fruits, during the postharvest, harvested at different ripening stages. Scientia Horticulturae. 2018; 227 ():10-21.
Chicago/Turabian StyleJessica Tosin Milanez; Leandro Camargo Neves; Ronan Carlos Colombo; Muhammad Shahab; Sergio Ruffo Roberto. 2018. "Bioactive compounds and antioxidant activity of buriti fruits, during the postharvest, harvested at different ripening stages." Scientia Horticulturae 227, no. : 10-21.