Hypericum perforatum L. (St. John’s wort) is a medicinal plant known for its diverse secondary metabolites and pharmacological potential. This study provides a comprehensive evaluation of the phytochemical composition and bioactivities of methanolic extracts from wild H. perforatum. LC-MS/MS analysis identified 36 compounds, including phenolic acids (chlorogenic acid, protocatechuic acid) and flavonoids (rutin, isoquercitrin, quercetin), with the first-time detection of genkwanin, vicenin-2, schaftoside, and afzelin, to the best of our knowledge. The extract demonstrated significant antioxidant activity, with a total phenolic content of 203.04 mg GAE/g DW and strong radical scavenging activity in DPPH (544.78 mg TE/g DW) and ABTS (312.15 mg TE/g DW) assays. In vitro assays showed marked cytotoxicity across multiple cancer cell lines, with IC50 values as low as 3.57 µg/mL in A549 cells, indicating stronger activity compared to cisplatin. Flow cytometric analysis revealed substantial apoptosis induction (up to 53.67% in A549 cells) and G0/G1 cell cycle arrest (76.37% in A549). The extract also exhibited moderate antibacterial activity against E. coli and S. aureus, but no activity against P. aeruginosa or Candida species. These results position H. perforatum as a rich source of bioactive compounds with antioxidant, anticancer, and antimicrobial potential, and suggest further research into its therapeutic applications.

Cyclotrichium (Lamiaceae) is reported as a new host genus for the rust fungus, Puccinia menthae. The morphological and microscopical characteristics of this fungus are described and illustrated based on the collected materials.

İstanbul Üniversitesi | Tarihten Geleceğe Bilim Köprüsü - 1453

Professional self-competence and self-efficacy of paramedics who first interfere to emergency events are important. An important ratio of deaths in emergency intervention are due to emergencies that require cardiopulmonary resuscitation (CPR), and also emergency labor is frequently seen in rural areas. In this research, CPR and labor simulation training’s effect on professional self-competence and relevance of self-efficacy in paramedic students has been investigated. The pre-test and post-test experimental research was carried out with 68 pregraduate paramedic students. Following the pre-questionnaire, all students were given simulation training about CPR and labor, and students were evaluated two times with a weak interval by making one-to-one simulation applications. Afterwards a post-questionnaire was applied, Sherer’s General Self-Efficacy Scale (SGSES) was used to determine self-efficacy levels. Professional self-competence perception level and evaluation scores of the students increased significantly in pre and post-questionnaire comparison. The statistically significant difference between professional self-competence perception and graduated high-school in pre-questionnaire was not obtained in post-questionnaire. While SGSES scores were higher in the students who felt more professional self-competence, SGSES scores and professional self-competence scores did not statistically differ among pre and post-questionnaires. Simulation trainings in the education process of paramedic students has significant impact on professional self-competence.