We investigated the crystallization of bio-nylon 54 monomer salt in terms of the solid–liquid equilibrium, crystal structure, thermal behaviors and crystallization mode.

Polyamide 56 is regarded as one of the most promising materials for the textile industry. This report gives the crystallization route of high-quality polyamide 56 monomers, and its crystal structure, transformation behaviors, and solubility.

Galactooligosaccharides (GOS) are one of the most important functional oligosaccharide prebiotics. The surface display of enzymes was considered one of the most excellent strategies to obtain these products. However, a rough industrial environment would affect the biocatalytic process. The catalytic process could be efficiently improved using biofilm-based fermentation with high resistance and activity. Therefore, the combination of the surface display of β-galactosidase and biofilm formation in Pichia pastoris was constructed. The results showed that the catalytic conversion rate of GOS was up to 50.3% with the maximum enzyme activity of 5125 U/g by screening the anchorin, and the number of the continuous catalysis batches was up to 23 times. Thus, surface display based on biofilm-immobilized fermentation integrated catalysis and growth was a co-culture system, such that a dynamic equilibrium in the consolidated integrative process was achieved. This study provides the basis for developing biofilm-based surface display methods in P. pastoris during biochemical production processes.

A simple humidity process allows a direct solid–solid transformation from the solvate (methanol trihydrate of cAMPNa) to its hydrate form (pentahydrate).