Due to the influence of the complex underwater environment, the initial alignment method for Doppler velocity log (DVL)-aided strap-down inertial navigation systems (SINS) often suffer from performance degradation, especially when DVL measurements are contaminated by outliers. In this paper, an outlier-resistant Initial Alignment method with interference suppression for SINS/DVL integrated navigation system is proposed, by which, by constructing an improved Mahalanobis distance anomalous detection criterion, the anomaly of the residual vector composed of observation vectors is judged, and an adaptive weighting factor is introduced into the observation matrix to suppress the abnormal interference in the alignment process. Simulation and experimental results show that, compared with existing initial alignment methods, the proposed method achieves higher alignment accuracy in the presence of outliers, which is more suitable for the SINS/DVL integrated navigation system.