Limosilactobacillus reuteri, a recognized probiotic, improves intestinal health in animals, but the mechanism remains unclear. This study investigates the mechanisms by which L. reuteri ZY15, isolated from healthy pig feces, mitigates intestinal barrier damage and inflammation caused by oxidative stress in Enterotoxigenic Escherichia coli (ETEC) K88-challenged mice. The results indicated that L. reuteri ZY15 increased antioxidant capacity by reducing serum reactive oxygen species (ROS) and superoxide dismutase (SOD) levels. L. reuteri ZY15 enhanced the intestinal barrier by upregulating mucin 1, mucin 2, occludin, zonula occludens-1 (ZO-1), and claudin-1 expressions in protein and mRNA levels. It significantly alleviated intestinal inflammation by reducing the proinflammatory cytokines interleukin-1β (IL-1β), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), and interleukin-17 (IL-17) mRNA and protein levels. Notably, L. reuteri ZY15 suppressed intestinal inflammation by inhibiting AKT/mTOR/HIF-1α/RORγt/IL-17 pathway activation. Additionally, it significantly altered the structure of gut microorganisms by enriching Akkermansia and Clostridia_UCG.014, and thereby re-establishing colonization resistance and alleviating ETEC K88-induced intestinal barrier damage and inflammation in mice. Taken together, our findings reveal the protective mechanism of L. reuteri ZY15 in mice challenged with ETEC K88 by regulating AKT/mTOR/HIF-1α/RORγt/IL-17 signaling and microbial imbalance. Leveraging these properties, live L. reuteri ZY15 offers a promising alternative treatment for Escherichia coli-induced diarrhea in weaned piglets.

This study explored Trichoderma asperellum’s lignocellulose degradation potential in wheat straw (WS) and NaOH-treated WS via solid-state fermentation (SSF) over 30 days. Compared to the control, WS treated with T. asperellum (TW) and NaOH-treated WS with T. asperellum (TN) showed increased dry matter loss rates of 15.67 and 15.76%, respectively. Cellulose degradation reached 33.51 and 28.00%, while hemicellulose degradation increased to 31.56 and 63.86%. Crude protein (CP) content rose to 10.96 and 7.44%, and reducing sugar content to 10.86 and 12.41 mg/g, respectively. T. asperellum effectively reduced lignocellulose content and enhanced substrate nutrition, supporting subsequent uses of WS as fertilizer, feed, or for bioethanol production. Enzymatic activity and structural analyses were performed to further confirm the lignocellulose-degrading ability of T. asperellum and to analyze the degradation mechanisms. Transcriptomic analysis revealed that, compared with the control group, the TN group had 4,548, 4,399, and 6,051 differentially expressed genes (DEGs) at 5, 10, and 30 days, respectively, mainly involved in cellulose and hemicellulose degradation, carbohydrate metabolism, carbohydrate transport, glycoside hydrolases, and polysaccharide binding. T. asperellum can modify lignin by expressing dye-decolorizing peroxidase genes, and multiple key genes were identified for further research into its genetic regulation in lignocellulose degradation.

The recalcitrant structure of lignocellulose hinders the efficient valorization of wheat straw. White-rot fungi have been extensively studied for their abi

The white-rot fungus Irpex lacteus is recognized for its strong ligninolytic and polysaccharide-degrading capacity, but the key advantages in degrading lignocellulose and the regulation of its enzyme systems remain poorly understood. In this study, we identified a rich repertoire of carbohydrate-active enzymes in the genome of I. lacteus QJ. Relative to other white-rot fungi, an expanded glycoside hydrolase gene family in I. lacteus QJ suggesting strong potential for lignocellulose degradation. When I. lacteus QJ was cultivated on glucose or wheat straw for 4 and 8 days, wheat straw strongly induced carbohydrate-active enzyme genes on day 4, while ligninolytic enzyme genes exhibited delayed upregulation on day 8. The cellobiose dehydrogenase plays an important role in the degradation processes. Its expression pattern is consistent with that of cellulase, and it can support peroxidase activity by providing H₂O₂. These findings reveal temporal coordination between polysaccharide- and lignin-degrading enzymes, providing new theoretical ideas for the application of I. lacteus during the degradation process. Our results not only improve the mechanistic understanding of fungal lignocellulose deconstruction but also inform strategies to enhance biological pretreatment of agricultural residues for biorefinery applications.

Background/Objectives: Corn steep liquor Powder (CSLP) has previously been reported to promote fruity aroma production by Geotrichum candidum; however, the broader volatile profiles associated with CSLP-based cultivation relative to reference media remain insufficiently characterized. In this study, we compared the volatile metabolite and aroma-related profiles of G. candidum GC1 cultivated in a CSLP-based YMJ medium and two reference media, PDB and GAS. Methods: Using E-nose and HS-SPME-GC-MS, we compared the volatile profiles of G. candidum GC1 cultured in YMJ medium versus PDB and GAS media. Results: A total of 1414 volatile organic compounds (VOCs) belonging to 15 chemical classes were annotated. The YMJ group produced the highest VOC abundance, with significant enrichment of ketones, terpenoids, alcohols, pyrazines and phenols. Key compounds, such as 4-Nonenal (E) and 3(2H)-Furanone dihydro-2-methyl-, contributed fruity, sweet-bread-buttery-nutty notes, respectively, forming the complex floral-fruity-roasted aroma profile of G. candidum GC1 in YMJ medium. Volatile metabolomic analysis showed distinct medium-associated metabolic patterns, with the YMJ medium displaying a more diverse aroma-related volatile profile than the reference media under the conditions tested. Conclusions: The CSLP-based YMJ medium supported a distinct and relatively complex volatile profile in G. candidum GC1. These results support the potential use of CSLP-containing media in flavor-oriented fermentation design.