Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs). The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated. The ACPs were dried with hot air (HD), vacuum (VD) and freeze drying (FD) methods. The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained. HPLC analysis showed that the monosaccharide compositions of the three ACPs dried with HD, VD or FD techniques were identical. The three ACPs exhibited antioxidant abilities in a concentration-dependent manner. ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content. At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively. Therefore, FD was a good choice for the drying of Astragalus cicer L. polysaccharides.

A completely randomized design employing a 2 × 2 factorial experiment was designed in this study to evaluate the effects of in ovo injection of disaccharide (DS) and/or methionine (Met) on hatchability, growth performance, blood hematology, and serum antioxidant parameters in geese. A total of 600 fertilized geese’s eggs containing live embryo were randomly assigned into 4 groups with 6 replicates and 25 eggs per replicate. Factors in four groups comprised noninjection, DS injection (25 g/L maltose + 25 g/L sucrose + 7.5 g/L NaCl), Met injection (5 g/L Met + 7.5 g/L NaCl), or DS plus Met injection (25 g/L maltose + 25 g/L sucrose + 5 g/L Met + 7.5 g/L NaCl), respectively. We found that the administration of DS in embryo increased hatching time, yolk sac-free carcass weight, yolk sac-free carcass indexes and decreased assisted hatching ratio, yolk sac weight, yolk sac indexes, but did not affect hatchability and mortality. Moreover, higher body weight and serum glucose concentrations in DS injection group compared with noninjection group were observed on day of hatching. The body weight and average daily gain (ADG) of geese in DS injection group were higher than noninjection group after incubation. In ovo injection of Met increased hatching time and yolk sac-free carcass indexes, but decreased yolk sac indexes. In addition, the strategy of in ovo feeding of Met led to higher body weight, ADG, serum uric acid, glutathione (GSH), and glutathione peroxidase concentrations, as well as lower GSSG/GSH ratio, serum glutathione disulfide (GSSG), and malondialdehyde (MDA) concentrations than the noninjection group on day of hatching. The post-hatching body weight, ADG, serum total protein, albumin, and uric acid concentrations increased, whereas post-hatching serum GSSG and MDA concentrations and GSSG/GSH ratio decreased when injected with Met. In addition, synergistic effects of in ovo injection of DS plus Met on hatching time as well as post-hatching body weight and ADG were observed. Therefore, in ovo injection of DS plus Met was demonstrated to be a way to improve the development of geese during early incubation stages.

In geese breeding, due to the frequent influence of drugs and environmental and other factors, geese are extremely prone to oxidative stress, which adversely affects growth and development, geese meat quality, down production, and severely affects the development of the geese industry. Ferulic acid from plant extracts can be used as a feed additive, which is safe and non-toxic, and it can exert certain therapeutic effects on oxidative stress in geese. This experiment investigated the effect of ferulic acid on the growth performance, organs indices, and intestinal oxidative indices of Jilin white geese under lipopolysaccharide-induced oxidative stress. Geese were randomly divided into six groups: C (blank control), L (lipopolysaccharide control), F1 (60 mg/kg ferulic acid), F2 (120 mg/kg ferulic acid), F3 (180 mg/kg ferulic acid), and F4 (240 mg/kg ferulic acid). Groups L and F1–F4 were injected intraperitoneally with 0.5 mg/kg lipopolysaccharide and group C with an equivalent volume of normal saline on days 14,17 and 20, and 10 animals from each group were randomly selected for slaughter on day 21. The results showed that: 1) On day 14, the final body weight and average daily feed intake were significantly higher in group F3 than in group L, and on day 21, the final body weight was significantly higher in group F3 than in group L. 2) The thymus index was significantly higher in group F4 than in group L. 4) In the duodenum, MDA activity was reduced in group C compared with that in group L. 5) In the jejunum and ileum, MDA was significantly lower in group F3 than in group L. These results show that the addition of 180 mg/kg of ferulic acid to the diet can promote the growth of geese and alleviate the damage caused by oxidative stress in all intestinal segments.