Nanobubbles have emerged as a novel technology, yet their applications remain largely limited to cleaning and oxidation. This study explores the potential of ozone nanobubbles as a pretreatment method for liquefied wood. Wood meal was treated with ozone nanobubbles in tap water under three different conditions: room temperature, 50 °C, and room temperature followed by ultrasonic treatment. The treated samples were then compared with untreated wood meal through component analysis, FT-IR functional group evaluation, and X-Ray diffraction (XRD) analysis of cellulose crystallinity. In the liquefaction process, residue rates, FT-IR analysis, hydroxyl numbers, and viscosity were examined. Additionally, the mechanical properties of synthesized polyurea films were evaluated via tensile testing. The results showed a reduction in amorphous cellulose from 62.3% to 56.6% and hemicellulose from 42.8% to 35.9%, leading to liquefied wood with a high hydroxyl value from 341 KOH/mg to 387 KOH/mg and significantly lower viscosity from 684 cP to 264 cP. Furthermore, the polyurea films synthesized from the treated liquefied wood exhibited no deterioration in physical properties. These findings highlight ozone nanobubble pretreatment as a promising and industrially valuable process for producing low-residue, low-viscosity liquefied wood without compromising material performance.