The objective of the present study was to evaluate the external and organoleptic characters, microscopic, physico-chemical, chromatic and microbiological parameters of the honey from Myracrodruon urundeuva allemão (Anacardiceae - Aroeira) of the Jequitinhonha River Valley / Brazil, in order to create its geographical indication. In the first stage a data survey was carried out to characterize the production of honey in the Jequitinhonha Valley. In order to evaluate the use of land as well as the forms of agriculture in relation to beekeeping. For characterization of the aroeira honey of the area, the microscopic, physico-chemical and microbiological quality of the honey was evaluated in samples collected in AAPIVAJE. Samples were collected for convenience. Honey production did not correlate with the area occupied, in hectare, by none of the types of land use in the middle region of the Jequitinhonha River Valley. There was also no significant correlation between honey production and the form of agriculture. In general, all samples of honey analyzed had satisfactory physico-chemical characteristics in terms of parameters of sugars, moisture, ashes, pH, acidity, color, taste and consistency. The analyzed samples were free of commercial sugar and dyes and present diastase activity. In this study we can see that all honey samples had a low microbial count of coliforms at 30°C and coliforms at 45°C (thermotolerant), indicating good hygienic-sanitary quality of the honey extraction and processing procedures. In the same way, it was observed that the counts of fungi and yeasts of all the honey were within the recommended parameters in the legislation of Apis. The presence of Salmonella was not detected in any sample of aroeira honey. This study will contribute to the geographical indication for aroeira honey, produced only in the middle region of the Jequitinhonha River Valley. Key words: Apis mellífera, honey quality, sugars, diastase enzyme, moisture, ashes, acidity, microbial.

The generation of large volumes of waste by industrial processes has become an object of study because of the necessity to characterize the composition of residues in order to suggest appropriate treatments and to minimize adverse environmental impacts. We performed analyses of total fixed and volatile solids, moisture, and chemical oxygen demand (COD). We found high organic matter content. We also measured physicochemical characteristics, including corrosivity, reactivity, and toxicity. Sewage sludge showed levels of chloride and sodium above the maximum allowed limits. These data suggest the potential for anaerobic digestion as a treatment option for sewage sludge and for its use as a biofertilizer.

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This study aimed to investigate, in oral squamous carcinoma cells (OSCC), the antineoplastic effect of Apis mellifera honey produced from Aroeira and wildflower, in the region of Jequitinhonha River Valley, Brazil. Furthermore, we investigate the molecular mechanism of honey performance through bioinformatic analysis. Honey and OSCC-related gene list were screened using GeneCards. The online tool STRING was used to identify the potential interaction networks of protein products of these genes. The genes with the highest weighted number of links (WNL) were called leader genes. GO and KEGG pathway analysis was performed to classify the genes. We use a search tool STITCH for interactions of chemicals-proteins. The chemical characterization of honey and its antiproliferative and antimigratory effects in SCC-9 cell lines was evaluated in vitro. The analysis of the interaction network obtained from the STRING database indicated by the following leader genes: TNF, IL6 and HDAC1. GO analysis for the honey, and OSCC-related proteins revealed that leader genes-regulated cancers genes were markedly enriched in some functions. Through the chemical characterization, the bioactive classes: steroids/triterpenoids, flavonoids, saponins, alkaloids, and tannins were identified. Polyphenols act in activating CASP3 and TP53 and inhibit the action of DNMT1. The honey produced from Aroeira and wildflower reduced proliferative phenotype and migration of OSCC cells. Key words: Aroeira honey, Myracrodruon urundeuva, wild honey, Apis mellifera, therapeutic, Jequitinhonha River Valley.