The time spent on this new procedure is no more than 3 hours, which is 6 to 12 times faster than the most widely used methods. This approach makes it possible to obtain samples suitable for subsequent analysis by any analytical instrumental method.

Microplastics, defined as particles up to 5 mm in size, present a significant environmental and health concern due to their ubiquity, capacity to accumulate in organisms, and potential to cause toxic effects, inflammation, and endocrine disruption. A major challenge in addressing this issue is the lack of a universal method for sample preparation and analysis across different environmental matrices. This study addresses this gap by applying a custom-developed method for isolating microplastics from freshwater, followed by a comparative analysis of their abundance using three techniques: spectral (μ-FTIR) and thermal (TGA and pyro-GC-MS). The study was conducted on water samples from the Ob River near Novosibirsk, a major industrial center in Siberia. Field processing entailed filtering 20 L water volumes through a polyamide fabric with a nominal 100 µm pore size. Subsequent characterization established that the entire population of detected particles fell within the 100 to 500 µm interval. The results revealed microplastic concentrations of 0–10,000 particles/m³ (μ-FTIR), 6–19 mg/m³ (TGA), and 0.47–2.96 mg/m³ (pyro-GC-MS). Critically, the data showed spatially variable contamination, with higher microplastic levels identified near industrial wastewater discharge stations and urban recreational areas.