The NAC transcription factor family enhances plant adaptation to environmental challenges by participating in signalling pathways triggered by abiotic stressors and hormonal cues. We identified 69 NAC genes in the Eucommia ulmoides genome and renamed them according to their chromosomal distribution. These EuNAC proteins were clustered into 13 sub-families and distributed on 16 chromosomes and 2 scaffolds. The gene structures suggested that the number of exons varied from two to eight among these EuNACs, with a multitude of them containing three exons. Duplicated events resulted in a large gene family; 12 and four pairs of EuNACs were the result of segmental and tandem duplicates, respectively. The drought-stress response pattern of 12 putative EuNACs was observed under drought treatment, revealing that these EuNACs could play crucial roles in mitigating the effects of drought stress responses and serve as promising candidate genes for genetic engineering aimed at enhancing the drought stress tolerance of E. ulmoides. This study provides insight into the evolution, diversity, and characterisation of NAC genes in E. ulmoides and will be helpful for future characterisation of putative EuNACs associated with water deficit.

Rhizosphere soil microorganisms are significant factors affecting plant growth, especially that of saline–alkali tolerant plants in the desert ecosystem. We performed high-throughput sequencing in order to identifying the fungal community structures and their relationships to the physicochemical properties of different soil layers for the desert plant, Anabasis aphylla, in its natural environment. The number of unique operational taxonomic units (OTUs) found in the bulk soil of the 0–20 cm layer contributed to the biggest percentage (24.13%) of the overall amount of unique OTUs. Despite the fact that there was a rather large variety of fungi in the bulk soil of A. aphylla, the number of dominating fungi, which included Ascomycota, Microascus, and Arachnomyces, was found to be in quite high abundance in the rhizosphere soil. In the 20–40 cm layer of rhizosphere soil, the phylum Ascomycota accounted for 84.78% of the total phyla identified, whereas the species Microascus and Arachnomyces accounted for 24.72% and 37.18%, respectively, of the total species identified. In terms of the soil physicochemical properties, electric conductivity was the primary environmental component influencing the dominant fungi. The findings of this research enhance our comprehension of dominant fungi distributions and relevant environmental factors affecting the saline–alkali tolerant desert plant, A. aphylla. The results also provide a theoretical basis to help elucidate fungi adaptation mechanisms to the saline–alkali environment and methods for their isolation and screening.