Victor Hugo Aquino

Dr. Victor Hugo Aquino

Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Central, Paraguay
orcid
Scott Weaver

Research Focus & Metrics

Academic Career Length

26Years (2001-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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27 January 2010 in Virology Journal
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Journal Article
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TPTelma R Poloni
Telma R Poloni
AOAnibal S Oliveira
Anibal S Oliveira
HAHelda L Alfonso
Helda L Alfonso
LGLarissa R Galvão
Larissa R Galvão
AAAlberto A Amarilla
Alberto A Amarilla
Victor Hugo AquinoVictor Hugo Aquino
Victor Hugo Aquino
Early diagnosis of dengue virus (DENV) infection is important for patient management and control of dengue outbreaks. The objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of DENV infection by real time RT-PCR. Two febrile patients, who have been attended at the General Hospital of the School of Medicine of Ribeirao Preto, Sao Paulo University were included in the study. Serum, urine and saliva samples collected from both patients were subjected to real time RT-PCR for DENV detection and quantification. Dengue RNA was detected in serum, urine and saliva samples of both patients. Patient 1 was infected with DENV-2 and patient 2 with DENV-3. Data presented in this study suggest that urine and saliva could be used as alternative samples for early diagnosis of dengue virus infection when blood samples are difficult to obtain, e.g., in newborns and patients with hemorrhagic syndromes.

1 July 2006 in Microbes and Infection
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Journal Article
Closed access
ABAlessandra A. Borges
Alessandra A. Borges
GCGelse M. Campos
Gelse M. Campos
Marcos Lazaro MoreliMarcos Lazaro Moreli
Marcos Lazaro Moreli
RSRicardo Luiz Moro De Sousa
Ricardo Luiz Moro De Sousa
Victor Hugo AquinoVictor Hugo Aquino
Victor Hugo Aquino
LFLuiz T.M. Figueiredo
Luiz T.M. Figueiredo

12 July 2010 in Virology Journal
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Journal Article
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MFMario Lg de Figueiredo
Mario Lg de Figueiredo
AGAlmério de C Gomes
Almério de C Gomes
AAAlberto A Amarilla
Alberto A Amarilla
ALAndré de S Leandro
André de S Leandro
AOAgnaldo de S Orrico
Agnaldo de S Orrico
LFLuiz Tadeu Figueiredo
Luiz Tadeu Figueiredo
Dengue epidemics have been reported in Brazil since 1985. The scenery has worsened in the last decade because several serotypes are circulating and producing a hyper-endemic situation, with an increase of DHF/DSS cases as well as the number of fatalities. Herein, we report dengue virus surveillance in mosquitoes using a Flavivirus genus-specific RT-Hemi-Nested-PCR assay. The mosquitoes (Culicidae, n = 1700) collected in the Northeast, Southeast and South of Brazil, between 1999 and 2005, were grouped into 154 pools. Putative genomes of DENV-1, -2 and -3 were detected in 6 mosquito pools (3.8%). One amplicon of putative DENV-1 was detected in a pool of Haemagogus leucocelaenus suggesting that this virus could be involved in a sylvatic cycle. DENV-3 was found infecting 3 pools of larvae of Aedes albopictus and the nucleotide sequence of one of these viruses was identified as DENV-3 of genotype III, phylogenetically related to other DENV-3 isolated in Brazil. This is the first report of a nucleotide sequence of DENV-3 from larvae of Aedes albopictus.

10 November 2014 in PLOS ONE
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Journal Article
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VMVanessa Danielle Muller
Vanessa Danielle Muller
RSRicardo O. S. Soares
Ricardo O. S. Soares
NSNilton Nascimento Dos Santos
Nilton Nascimento Dos Santos
ATAmanda Cristina Trabuco
Amanda Cristina Trabuco
ACAdelia Cristina Cintra
Adelia Cristina Cintra
Victor Hugo AquinoVictor Hugo Aquino
Victor Hugo Aquino
The Flaviviridae family includes several virus pathogens associated with human diseases worldwide. Within this family, Dengue virus is the most serious threat to public health, especially in tropical and sub-tropical regions of the world. Currently, there are no vaccines or specific antiviral drugs against Dengue virus or against most of the viruses of this family. Therefore, the development of vaccines and the discovery of therapeutic compounds against the medically most important flaviviruses remain a global public health priority. We previously showed that phospholipase A2 isolated from the venom of Crotalus durissus terrificus was able to inhibit Dengue virus and Yellow fever virus infection in Vero cells. Here, we present evidence that phospholipase A2 has a direct effect on Dengue virus particles, inducing a partial exposure of genomic RNA, which strongly suggests inhibition via the cleavage of glycerophospholipids at the virus lipid bilayer envelope. This cleavage might induce a disruption of the lipid bilayer that causes a destabilization of the E proteins on the virus surface, resulting in inactivation. We show by computational analysis that phospholipase A2 might gain access to the Dengue virus lipid bilayer through the pores found on each of the twenty 3-fold vertices of the E protein shell on the virus surface. In addition, phospholipase A2 is able to inactivate other enveloped viruses, highlighting its potential as a natural product lead for developing broad-spectrum antiviral drugs.

1 March 2012 in Toxicon
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Journal Article
Closed access
VMVanessa Danielle Menjon Muller
Vanessa Danielle Menjon Muller
RRRaquel Rinaldi Russo
Raquel Rinaldi Russo
ACAdelia Cristina Oliveira Cintra
Adelia Cristina Oliveira Cintra
MSMarco Aurelio Sartim
Marco Aurelio Sartim
RARaquel De Melo Alves-Paiva
Raquel De Melo Alves-Paiva
Victor Hugo AquinoVictor Hugo Aquino
Victor Hugo Aquino