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The nucleotide polymorphisms (SNPs) associated with the biofilm formation phenotype of Helicobacter pylori were investigated. Fifty-six H. pylori isolates from Bangladeshi patients were included in this cross-sectional study. Crystal violet was used to classify the phenotypes into high- and low-biofilm formers. Whole genome sequences were analyzed using the “Antimicrobial Resistance Identification By Assembly” (ARIBA) pipeline. The results indicated 19.6% high- and 81.4% low-biofilm formers. These phenotypes were not related to specific clades in the phylogenetic analysis. Biofilm formation was significantly associated with SNPs of alpA, alpB, cagE, cgt, csd4, csd5, futB, gluP, homD, and murF (P < 0.05). Among the SNPs reported in alpB, strains encoding the N156K, G160S, and A223V mutations were high-biofilm formers. Mutations associated with antibiotic resistance can be detected. This study revealed the potential role of SNPs to biofilm formation, and propose a method to detect mutation in antibiotic resistance and biofilm from whole genome sequences.
Kartika Afrida Fauzia; Hafeza Aftab; Muhammad Miftahussurur; Langgeng Agung Waskito; Vo Phuoc Tuan; Takashi Matsumoto; Michiyuki Yurugi; Phawinee Subsomwong; Evariste Tshibangu-Kabamba; Junko Akada; Yoshio Yamaoka. Genetic Determinants of Biofilm Formation and Antibiotic Resistance of Helicobacter Pylori using Whole Genome Sequencing. 2021, 1 .
AMA StyleKartika Afrida Fauzia, Hafeza Aftab, Muhammad Miftahussurur, Langgeng Agung Waskito, Vo Phuoc Tuan, Takashi Matsumoto, Michiyuki Yurugi, Phawinee Subsomwong, Evariste Tshibangu-Kabamba, Junko Akada, Yoshio Yamaoka. Genetic Determinants of Biofilm Formation and Antibiotic Resistance of Helicobacter Pylori using Whole Genome Sequencing. . 2021; ():1.
Chicago/Turabian StyleKartika Afrida Fauzia; Hafeza Aftab; Muhammad Miftahussurur; Langgeng Agung Waskito; Vo Phuoc Tuan; Takashi Matsumoto; Michiyuki Yurugi; Phawinee Subsomwong; Evariste Tshibangu-Kabamba; Junko Akada; Yoshio Yamaoka. 2021. "Genetic Determinants of Biofilm Formation and Antibiotic Resistance of Helicobacter Pylori using Whole Genome Sequencing." , no. : 1.
Microbiome, the study of microbial communities in specific environments, has developed significantly since the Human Microbiome Project began. Microbiomes have been associated with changes within environmental niches and the development of various diseases. The development of high-throughput technology such as next-generation sequencing has also allowed us to perform transcriptome studies, which provide accurate functional profiling data. Metabolome studies, which analyse the metabolites found in the environment, are the most direct environmental condition indicator. Although each dataset provides valuable information on its own, the integration of multiple datasets provides a deeper understanding of the relationship between the host, agent and environment. Therefore, network analysis using multiple datasets might give a clearer understanding of disease pathogenesis.
Dalla Doohan; Yudith Annisa Ayu Rezkitha; Langgeng Agung Waskito; Ratha-Korn Vilaichone; Yoshio Yamaoka; Muhammad Miftahussurur. Integrating microbiome, transcriptome and metabolome data to investigate gastric disease pathogenesis: a concise review. Expert Reviews in Molecular Medicine 2021, 23, 1 .
AMA StyleDalla Doohan, Yudith Annisa Ayu Rezkitha, Langgeng Agung Waskito, Ratha-Korn Vilaichone, Yoshio Yamaoka, Muhammad Miftahussurur. Integrating microbiome, transcriptome and metabolome data to investigate gastric disease pathogenesis: a concise review. Expert Reviews in Molecular Medicine. 2021; 23 ():1.
Chicago/Turabian StyleDalla Doohan; Yudith Annisa Ayu Rezkitha; Langgeng Agung Waskito; Ratha-Korn Vilaichone; Yoshio Yamaoka; Muhammad Miftahussurur. 2021. "Integrating microbiome, transcriptome and metabolome data to investigate gastric disease pathogenesis: a concise review." Expert Reviews in Molecular Medicine 23, no. : 1.
The use of serum anti-Helicobacter pylori IgG and pepsinogen (PG) detection as a diagnostic method was evaluated in Sri Lanka. Gastric biopsies were performed (353 patients), and the prevalence of H. pylori infection was 1.7% (culture) and 2.0% (histology). IgG serology testing showed an area under the curve (AUC) of 0.922 (cut-off, 2.95 U/mL; specificity, 91.56%; sensitivity, 88.89%). Histological evaluation showed mild atrophy (34.3%), moderate atrophy (1.7%), metaplasia (1.7%), chronic gastritis (6.2%), and normal tissue (56%). The PGI/PGII ratio was significantly higher in H. pylori-negative patients (p< 0.01). PGII and PGI/PGII levels were lower in patients with metaplasia than in those with normal mucosa (p = 0.049 and p< 0.001, respectively). The PGI/PGII ratio best discriminated metaplasia and moderate atrophy (AUC 0.88 and 0.76, respectively). PGI and PGII alone showed poor discriminative ability, especially in mild atrophy (0.55 and 0.53, respectively) and chronic gastritis (0.55 and 0.53, respectively). The best cut-off to discriminate metaplasia was 3.25 U/mL (95.19% specificity, 83.33% sensitivity). Anti-H. pylori IgG and PG assessment (ABC method) was performed (group B, 2.0%; group A, 92.1%). The new cut-off more accurately identified patients with metaplasia requiring follow-up (group B, 5.4%). Assessment of anti-H. pylori IgG and PG is valuable in countries with a low prevalence of H. pylori infection.
Dalla Doohan; Kartika Fauzia; Jeewantha Rathnayake; Meegahalande Lamawansa; Langgeng Waskito; Vo Tuan; Azzaya Dashdorj; Evariste Kabamba; Bui Phuc; Shamshul Ansari; Junko Akada; Takashi Matsumoto; Tomohisa Uchida; Takeshi Matsuhisa; Yoshio Yamaoka. Pepsinogen and Serum IgG Detection Is a Valuable Diagnostic Method for Helicobacter pylori Infection in a Low-Prevalence Country: A Report from Sri Lanka. Diagnostics 2021, 11, 1364 .
AMA StyleDalla Doohan, Kartika Fauzia, Jeewantha Rathnayake, Meegahalande Lamawansa, Langgeng Waskito, Vo Tuan, Azzaya Dashdorj, Evariste Kabamba, Bui Phuc, Shamshul Ansari, Junko Akada, Takashi Matsumoto, Tomohisa Uchida, Takeshi Matsuhisa, Yoshio Yamaoka. Pepsinogen and Serum IgG Detection Is a Valuable Diagnostic Method for Helicobacter pylori Infection in a Low-Prevalence Country: A Report from Sri Lanka. Diagnostics. 2021; 11 (8):1364.
Chicago/Turabian StyleDalla Doohan; Kartika Fauzia; Jeewantha Rathnayake; Meegahalande Lamawansa; Langgeng Waskito; Vo Tuan; Azzaya Dashdorj; Evariste Kabamba; Bui Phuc; Shamshul Ansari; Junko Akada; Takashi Matsumoto; Tomohisa Uchida; Takeshi Matsuhisa; Yoshio Yamaoka. 2021. "Pepsinogen and Serum IgG Detection Is a Valuable Diagnostic Method for Helicobacter pylori Infection in a Low-Prevalence Country: A Report from Sri Lanka." Diagnostics 11, no. 8: 1364.
Helicobacter pylori is a pathogenic microorganism that successfully inhabits the human stomach, colonizing it by producing several virulence factors responsible for preventing host self-defense mechanisms. The adherence mechanism to gastric mucosal tissue is one of the most important processes for effective colonization in the stomach. The blood group antigen-binding adhesion (BabA) and sialic acid-binding adherence (SabA) are two H. pylori outer membrane proteins able to interact with antigens in the gastroduodenal tract. H. pylori possesses several mechanisms to control the regulation of both BabA and SabA in either the transcriptional or translational level. BabA is believed to be the most important protein in the early infection phase due to its ability to interact with various Lewis antigens, whereas SabA interaction with sialylated Lewis antigens may prove important for the adherence process in the inflamed gastric mucosal tissue in the ongoing-infection phase. The adherence mechanisms of BabA and SabA allow H. pylori to anchor in the gastric mucosa and begin the colonization process.
Dalla Doohan; Yudith Rezkitha; Langgeng Waskito; Yoshio Yamaoka; Muhammad Miftahussurur. Helicobacter pylori BabA–SabA Key Roles in the Adherence Phase: The Synergic Mechanism for Successful Colonization and Disease Development. Toxins 2021, 13, 485 .
AMA StyleDalla Doohan, Yudith Rezkitha, Langgeng Waskito, Yoshio Yamaoka, Muhammad Miftahussurur. Helicobacter pylori BabA–SabA Key Roles in the Adherence Phase: The Synergic Mechanism for Successful Colonization and Disease Development. Toxins. 2021; 13 (7):485.
Chicago/Turabian StyleDalla Doohan; Yudith Rezkitha; Langgeng Waskito; Yoshio Yamaoka; Muhammad Miftahussurur. 2021. "Helicobacter pylori BabA–SabA Key Roles in the Adherence Phase: The Synergic Mechanism for Successful Colonization and Disease Development." Toxins 13, no. 7: 485.
Purpose: Histopathology method is often used as a gold standard diagnostic for Helicobacter pylori infection in Indonesia. However, it requires an endoscopic procedure which is limited in Indonesia. A non-invasive method, such as 14C Urea Breath Test (UBT), is more favorable; however, this particular method has not been validated yet. Patients and Methods: A total of 55 dyspeptic patients underwent gastroscopy and 14C-UBT test. We used Heliprobe® UBT for UBT test. As for the histology, May-Giemsa staining of two gastric biopsies (from the antrum and corpus) were evaluated following the Updated Sydney System. Results: The Receiver Operating Characteristics analysis showed that the optimum cut-off value was 57 with excellence Area under Curve = 0.955 (95% CI = 0.861– 1.000). By applying the optimum cut-off value, Heliprobe® UBT showed 92.31% for sensitivity, 97.62% for specificity, 92.31% for positive predictive value, 97.62% for negative predictive value, 38.77 for positive likelihood ratio, 0.0788 for negative likelihood ratio, and 96.36% for the accuracy. Conclusion: The 14C-UBT is an accurate test for H. pylori diagnosis with excellent sensitivity, specificity, and accuracy. The different optimum cut-off points suggested that a validation is absolutely necessary for new test prior application to the new population.
Muhammad Miftahussurur; Adinta Windia; Ari Fahrial Syam; Iswan Abbas Nusi; Ricky Indra Alfaray; Kartika Afrida Fauzia; Hartono Kahar; Herry Purbayu; Titong Sugihartono; Poernomo Boedi Setiawan; Ummi Maimunah; Ulfa Kholili; Husin Thamrin; Amie Vidyani; Dalla Doohan; Langgeng Agung Waskito; Yudith Annisa Ayu Rezkitha; Gontar Alamsyah Siregar; Yoshio Yamaoka. Diagnostic Value of 14C Urea Breath Test for Helicobacter pylori Detection Compared by Histopathology in Indonesian Dyspeptic Patients. Clinical and Experimental Gastroenterology 2021, ume 14, 291 -296.
AMA StyleMuhammad Miftahussurur, Adinta Windia, Ari Fahrial Syam, Iswan Abbas Nusi, Ricky Indra Alfaray, Kartika Afrida Fauzia, Hartono Kahar, Herry Purbayu, Titong Sugihartono, Poernomo Boedi Setiawan, Ummi Maimunah, Ulfa Kholili, Husin Thamrin, Amie Vidyani, Dalla Doohan, Langgeng Agung Waskito, Yudith Annisa Ayu Rezkitha, Gontar Alamsyah Siregar, Yoshio Yamaoka. Diagnostic Value of 14C Urea Breath Test for Helicobacter pylori Detection Compared by Histopathology in Indonesian Dyspeptic Patients. Clinical and Experimental Gastroenterology. 2021; ume 14 ():291-296.
Chicago/Turabian StyleMuhammad Miftahussurur; Adinta Windia; Ari Fahrial Syam; Iswan Abbas Nusi; Ricky Indra Alfaray; Kartika Afrida Fauzia; Hartono Kahar; Herry Purbayu; Titong Sugihartono; Poernomo Boedi Setiawan; Ummi Maimunah; Ulfa Kholili; Husin Thamrin; Amie Vidyani; Dalla Doohan; Langgeng Agung Waskito; Yudith Annisa Ayu Rezkitha; Gontar Alamsyah Siregar; Yoshio Yamaoka. 2021. "Diagnostic Value of 14C Urea Breath Test for Helicobacter pylori Detection Compared by Histopathology in Indonesian Dyspeptic Patients." Clinical and Experimental Gastroenterology ume 14, no. : 291-296.
Helicobacter pylori is a major human pathogen for which increasing antibiotic resistance constitutes a serious threat to human health. Molecular mechanisms underlying this resistance have been intensively studied and are discussed in this Review. Three profiles of resistance — single drug resistance, multidrug resistance and heteroresistance — seem to occur, probably with overlapping fundamental mechanisms and clinical implications. The mechanisms that have been most studied are related to mutational changes encoded chromosomally and disrupt the cellular activity of antibiotics through target-mediated mechanisms. Other biological attributes driving drug resistance in H. pylori have been less explored and this could imply more complex physiological changes (such as impaired regulation of drug uptake and/or efflux, or biofilm and coccoid formation) that remain largely elusive. Resistance-related attributes deployed by the pathogen cause treatment failures, diagnostic difficulties and ambiguity in clinical interpretation of therapeutic outcomes. Subsequent to the increasing antibiotic resistance, a substantial drop in H. pylori treatment efficacy has been noted globally. In the absence of an efficient vaccine, enhanced efforts are needed for setting new treatment strategies and for a better understanding of the emergence and spread of drug-resistant bacteria, as well as for improving diagnostic tools that can help optimize current antimicrobial regimens. Increasing levels of antibiotic resistance in Helicobacter pylori are a serious threat to human health globally. This Review discusses H. pylori infection and antibiotic resistance, and provides insights into the underlying mechanisms and clinical implications (including detection and management).
Evariste Tshibangu-Kabamba; Yoshio Yamaoka. Helicobacter pylori infection and antibiotic resistance — from biology to clinical implications. Nature Reviews Gastroenterology & Hepatology 2021, 18, 613 -629.
AMA StyleEvariste Tshibangu-Kabamba, Yoshio Yamaoka. Helicobacter pylori infection and antibiotic resistance — from biology to clinical implications. Nature Reviews Gastroenterology & Hepatology. 2021; 18 (9):613-629.
Chicago/Turabian StyleEvariste Tshibangu-Kabamba; Yoshio Yamaoka. 2021. "Helicobacter pylori infection and antibiotic resistance — from biology to clinical implications." Nature Reviews Gastroenterology & Hepatology 18, no. 9: 613-629.
Although millions of people have been infected by Helicobacter pylori (H. pylori), only a small proportion of infected individuals will develop adverse outcomes, ranging from chronic gastritis to gastric cancer. Advanced development of the disease has been well-linked with chronic inflammation, which is significantly impacted by the adaptive and humoral immunity response. From the perspective of cellular immunity, this review aims to clarify the intricate axis between IL-17, IL-21, and IL-23 in H. pylori-related diseases and the pathogenesis of inflammatory gastrointestinal diseases. CD4+ helper T (Th)-17 cells, with the hallmark pleiotropic cytokine IL-17, can affect antimicrobial activity and the pathogenic immune response in the gut environment. These circumstances cannot be separated, as the existence of affiliated cytokines, including IL-21 and IL-23, help maintain Th17 and accommodate humoral immune cells. Comprehensive understanding of the dynamic interaction between molecular host responses in H. pylori-related diseases and the inflammation process may facilitate further development of immune-based therapy.
Astri Dewayani; Kartika Fauzia; Ricky Alfaray; Langgeng Waskito; Dalla Doohan; Yudith Rezkitha; Abdurachman Abdurachman; Takashi Kobayashi; Reny I’Tishom; Yoshio Yamaoka; Muhammad Miftahussurur. The Roles of IL-17, IL-21, and IL-23 in the Helicobacter pylori Infection and Gastrointestinal Inflammation: A Review. Toxins 2021, 13, 315 .
AMA StyleAstri Dewayani, Kartika Fauzia, Ricky Alfaray, Langgeng Waskito, Dalla Doohan, Yudith Rezkitha, Abdurachman Abdurachman, Takashi Kobayashi, Reny I’Tishom, Yoshio Yamaoka, Muhammad Miftahussurur. The Roles of IL-17, IL-21, and IL-23 in the Helicobacter pylori Infection and Gastrointestinal Inflammation: A Review. Toxins. 2021; 13 (5):315.
Chicago/Turabian StyleAstri Dewayani; Kartika Fauzia; Ricky Alfaray; Langgeng Waskito; Dalla Doohan; Yudith Rezkitha; Abdurachman Abdurachman; Takashi Kobayashi; Reny I’Tishom; Yoshio Yamaoka; Muhammad Miftahussurur. 2021. "The Roles of IL-17, IL-21, and IL-23 in the Helicobacter pylori Infection and Gastrointestinal Inflammation: A Review." Toxins 13, no. 5: 315.
Long-term infection of the stomach with Helicobacter pylori can cause gastric cancer. However, the mechanisms by which the bacteria adapt to the stomach environment are poorly understood. Here, we show that a small non-coding RNA of H. pylori (HPnc4160, also known as IsoB or NikS) regulates the pathogen’s adaptation to the host environment as well as bacterial oncoprotein production. In a rodent model of H. pylori infection, the genomes of bacteria isolated from the stomach possess an increased number of T-repeats upstream of the HPnc4160-coding region, and this leads to reduced HPnc4160 expression. We use RNA-seq and iTRAQ analyses to identify eight targets of HPnc4160, including genes encoding outer membrane proteins and oncoprotein CagA. Mutant strains with HPnc4160 deficiency display increased colonization ability of the mouse stomach, in comparison with the wild-type strain. Furthermore, HPnc4160 expression is lower in clinical isolates from gastric cancer patients than in isolates derived from non-cancer patients, while the expression of HPnc4160’s targets is higher in the isolates from gastric cancer patients. Therefore, the small RNA HPnc4160 regulates H. pylori adaptation to the host environment and, potentially, gastric carcinogenesis.
Ryo Kinoshita-Daitoku; Kotaro Kiga; Masatoshi Miyakoshi; Ryota Otsubo; Yoshitoshi Ogura; Takahito Sanada; Zhu Bo; Tuan Vo Phuoc; Tokuju Okano; Tamako Iida; Rui Yokomori; Eisuke Kuroda; Sayaka Hirukawa; Mototsugu Tanaka; Arpana Sood; Phawinee Subsomwong; Hiroshi Ashida; Tran Thanh Binh; Lam Tung Nguyen; Khien Vu Van; Dang Quy Dung Ho; Kenta Nakai; Toshihiko Suzuki; Yoshio Yamaoka; Tetsuya Hayashi; Hitomi Mimuro. A bacterial small RNA regulates the adaptation of Helicobacter pylori to the host environment. Nature Communications 2021, 12, 1 -12.
AMA StyleRyo Kinoshita-Daitoku, Kotaro Kiga, Masatoshi Miyakoshi, Ryota Otsubo, Yoshitoshi Ogura, Takahito Sanada, Zhu Bo, Tuan Vo Phuoc, Tokuju Okano, Tamako Iida, Rui Yokomori, Eisuke Kuroda, Sayaka Hirukawa, Mototsugu Tanaka, Arpana Sood, Phawinee Subsomwong, Hiroshi Ashida, Tran Thanh Binh, Lam Tung Nguyen, Khien Vu Van, Dang Quy Dung Ho, Kenta Nakai, Toshihiko Suzuki, Yoshio Yamaoka, Tetsuya Hayashi, Hitomi Mimuro. A bacterial small RNA regulates the adaptation of Helicobacter pylori to the host environment. Nature Communications. 2021; 12 (1):1-12.
Chicago/Turabian StyleRyo Kinoshita-Daitoku; Kotaro Kiga; Masatoshi Miyakoshi; Ryota Otsubo; Yoshitoshi Ogura; Takahito Sanada; Zhu Bo; Tuan Vo Phuoc; Tokuju Okano; Tamako Iida; Rui Yokomori; Eisuke Kuroda; Sayaka Hirukawa; Mototsugu Tanaka; Arpana Sood; Phawinee Subsomwong; Hiroshi Ashida; Tran Thanh Binh; Lam Tung Nguyen; Khien Vu Van; Dang Quy Dung Ho; Kenta Nakai; Toshihiko Suzuki; Yoshio Yamaoka; Tetsuya Hayashi; Hitomi Mimuro. 2021. "A bacterial small RNA regulates the adaptation of Helicobacter pylori to the host environment." Nature Communications 12, no. 1: 1-12.
CYP2C19 polymorphisms are important factors for proton pump inhibitor-based therapy. We examined the CYP2C19 genotypes and analyzed the distribution among ethnicities and clinical outcomes in Indonesia. We employed the polymerase chain reaction-restriction fragment length polymorphism method to determine the CYP2C19 genotypes and evaluated inflammation severity with the updated Sydney system. For CYP2C19*2, 46.4% were the homozygous wild-type allele, 14.5% were the homozygous mutated allele, and 39.2% were the heterozygous allele. For CYP2C19*3, 88.6% were the homozygous wild-type allele, 2.4% were the homozygous mutated allele, and 9.0% were the heterozygous allele. Overall, the prevalence of rapid, intermediate, and poor metabolizers in Indonesia was 38.5, 41.6, and 19.9%, respectively. In the poor metabolizer group, the frequency of allele *2 (78.8%) was higher than the frequency of allele *3 (21.2%). The Papuan had a significantly higher likelihood of possessing poor metabolizers than the Balinese (OR 11.0; P = 0.002). The prevalence of poor metabolizers was lower compared with the rapid and intermediate metabolizers among patients with gastritis and gastroesophageal reflux disease. Intermediate metabolizers had the highest prevalence, followed by rapid metabolizers and poor metabolizers. Dosage adjustment should therefore be considered when administering proton pump inhibitor-based therapy in Indonesia.
Muhammad Miftahussurur; Dalla Doohan; Ari Syam; Iswan Nusi; Phawinee Subsomwong; Langgeng Waskito; Hasan Maulahela; Fardah Akil; Willy Uwan; Gontar Siregar; Kartika Fauzia; Yudith Rezkitha; Abdul Rahman; I Wibawa; Alexander Saudale; Marselino Richardo; Titong Sugihartono; Alvi Chomariyati; Taufan Bramantoro; Tomohisa Uchida; Yoshio Yamaoka. CYP2C19 Polymorphisms in Indonesia: Comparison among Ethnicities and the Association with Clinical Outcomes. Biology 2021, 10, 300 .
AMA StyleMuhammad Miftahussurur, Dalla Doohan, Ari Syam, Iswan Nusi, Phawinee Subsomwong, Langgeng Waskito, Hasan Maulahela, Fardah Akil, Willy Uwan, Gontar Siregar, Kartika Fauzia, Yudith Rezkitha, Abdul Rahman, I Wibawa, Alexander Saudale, Marselino Richardo, Titong Sugihartono, Alvi Chomariyati, Taufan Bramantoro, Tomohisa Uchida, Yoshio Yamaoka. CYP2C19 Polymorphisms in Indonesia: Comparison among Ethnicities and the Association with Clinical Outcomes. Biology. 2021; 10 (4):300.
Chicago/Turabian StyleMuhammad Miftahussurur; Dalla Doohan; Ari Syam; Iswan Nusi; Phawinee Subsomwong; Langgeng Waskito; Hasan Maulahela; Fardah Akil; Willy Uwan; Gontar Siregar; Kartika Fauzia; Yudith Rezkitha; Abdul Rahman; I Wibawa; Alexander Saudale; Marselino Richardo; Titong Sugihartono; Alvi Chomariyati; Taufan Bramantoro; Tomohisa Uchida; Yoshio Yamaoka. 2021. "CYP2C19 Polymorphisms in Indonesia: Comparison among Ethnicities and the Association with Clinical Outcomes." Biology 10, no. 4: 300.
Although the type 4 secretion system of the integrating and conjugative elements (tfs ICE) is common in Helicobacter pylori, its clinical association with the cag pathogenicity island (cagPAI) have not yet been well-investigated. In this study, Vietnamese patient H. pylori samples (46 duodenal ulcer (DU), 51 non-cardia gastric cancer (NCGC), 39 chronic gastritis (CG)) were fully sequenced using next-generation sequencing and assembled into contigs. tfs3, tfs4, and cagPAI genes were compared with the public database. Most (94%) H. pylori strains possessed a complete cagPAI, which was the greatest risk factor for clinical outcomes, while the prevalences of tfs3 and tfs4 were 45% and 77%, respectively. Complete tfs3 and tfs4 were found in 18.3% and 17.6% of strains, respectively. The prevalence of H. pylori strains with complete tfs3 ICE in DU patients was significantly higher than that in NCGC patients (30.4% vs 11.7%, P < 0.05). In addition, the prevalence of strains with complete tfs3 ICE and cagPAI was significantly higher in DU patients than that in NCGC (28.4% vs 9.8%, P = 0.038) and CG patients (28.2% vs 7.7%, P = 0.024). cagPAI and complete tfs3 increased the risk of DU compared to NCGC (OR = 3.56, 95%CI: 1.1–14.1, P = 0.038) and CG (OR = 4.64, 95%CI: 1.1–27.6, P = 0.024). A complete cluster of tfs3 ICE was associated with gastroduodenal diseases in Vietnam. However, there was a low prevalence of the dupA/complete dupA cluster (15.4%) in the Vietnam strains. The prevalence of cagPAI in Vietnam strains was significantly higher than in US (P = 0.01) and Indonesia (P < 0.0001); the prevalence of the dupA cluster was also higher in the Vietnam strains than in the Indonesian strains (P < 0.05). In addition, the prevalence of ctkA, an accessory gene of tfs3, was significantly different between Vietnam and US strains (28% vs 2%, P = 0.0002). In summary, the acquisition of tfs3/4 ICE was common in H. pylori strains in patients with gastroduodenal disease in Vietnam, and the complete cluster of tfs3 ICE was a reliable marker for the severity of disease in the H. pylori infected population.
Bui Hoang Phuc; Vo Phuoc Tuan; Ho Dang Quy Dung; Tran Thanh Binh; Pham Huu Tung; Tran Dinh Tri; Ngo Phuong Minh Thuan; Vu Van Khien; Tran Thi Huyen Trang; Junko Akada; Takeshi Matsumoto; Yoshio Yamaoka. Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers. Scientific Reports 2021, 11, 1 -12.
AMA StyleBui Hoang Phuc, Vo Phuoc Tuan, Ho Dang Quy Dung, Tran Thanh Binh, Pham Huu Tung, Tran Dinh Tri, Ngo Phuong Minh Thuan, Vu Van Khien, Tran Thi Huyen Trang, Junko Akada, Takeshi Matsumoto, Yoshio Yamaoka. Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers. Scientific Reports. 2021; 11 (1):1-12.
Chicago/Turabian StyleBui Hoang Phuc; Vo Phuoc Tuan; Ho Dang Quy Dung; Tran Thanh Binh; Pham Huu Tung; Tran Dinh Tri; Ngo Phuong Minh Thuan; Vu Van Khien; Tran Thi Huyen Trang; Junko Akada; Takeshi Matsumoto; Yoshio Yamaoka. 2021. "Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers." Scientific Reports 11, no. 1: 1-12.
Background and Aim To determine the application range of diagnostic kits utilizing anti‐Helicobacter pylori antibody, we tested a newly developed latex aggregation turbidity assay (latex) and a conventional enzyme‐linked immunosorbent assay (E‐plate), both containing Japanese H. pylori protein lysates as antigens, using sera from seven Asian countries. Methods Serum samples (1797) were obtained, and standard H. pylori infection status and atrophy status were determined by culture and histology (immunohistochemistry) using gastric biopsy samples from the same individuals. The two tests (enzyme‐linked immunosorbent assay and latex) were applied, and receiver operating characteristics analysis was performed. Results Area under the curve (AUC) from the receiver operating characteristic of E‐plate and latex curves were almost the same and the highest in Vietnam. The latex AUC was slightly lower than the E‐plate AUC in other countries, and the difference became statistically significant in Myanmar and then Bangladesh as the lowest. To consider past infection cases, atrophy was additionally evaluated. Most of the AUCs decreased using this atrophy‐evaluated status; however, the difference between the two kits was not significant in each country, but the latex AUC was better using all samples. Practical cut‐off values were 3.0 U/mL in the E‐test and 3.5 U/mL in the latex test, to avoid missing gastric cancer patients to the greatest extent possible. Conclusions The kits were applicable in all countries, but new kits using regional H. pylori strains are recommended for Myanmar and Bangladesh. Use of a cut‐off value lower than the best cut‐off value is essential for screening gastric cancer patients.
Junko Akada; Evariste Tshibangu‐Kabamba; Vo Phuoc Tuan; Shusaku Kurogi; Yuichi Matsuo; Shamshul Ansari; Dalla Doohan; Bui Hoang Phuc; Phawinee Subsomwong; Langgeng Agung Waskito; Tran Thanh Binh; Lam Tung Nguyen; Vu Van Khien; Ho Dang Quy Dung; Muhammad Miftahussurur; Ari Fahrial Syam; Lotay Tshering; Ratha‐Korn Vilaichone; Varocha Mahachai; Thawee Ratanachu‐Ek; Pradeep Krishna Shrestha; Than Than Yee; Kyaw Htet; Hafeza Aftab; Takeshi Matsuhisa; Tomohisa Uchida; Tadayoshi Okimoto; Kazuhiro Mizukami; Masaaki Kodama; Kazunari Murakami; Naohiko Takahashi; Yoshio Yamaoka. Serum Helicobacter pylori antibody reactivity in seven Asian countries using an automated latex aggregation turbidity assay. Journal of Gastroenterology and Hepatology 2021, 36, 2198 -2209.
AMA StyleJunko Akada, Evariste Tshibangu‐Kabamba, Vo Phuoc Tuan, Shusaku Kurogi, Yuichi Matsuo, Shamshul Ansari, Dalla Doohan, Bui Hoang Phuc, Phawinee Subsomwong, Langgeng Agung Waskito, Tran Thanh Binh, Lam Tung Nguyen, Vu Van Khien, Ho Dang Quy Dung, Muhammad Miftahussurur, Ari Fahrial Syam, Lotay Tshering, Ratha‐Korn Vilaichone, Varocha Mahachai, Thawee Ratanachu‐Ek, Pradeep Krishna Shrestha, Than Than Yee, Kyaw Htet, Hafeza Aftab, Takeshi Matsuhisa, Tomohisa Uchida, Tadayoshi Okimoto, Kazuhiro Mizukami, Masaaki Kodama, Kazunari Murakami, Naohiko Takahashi, Yoshio Yamaoka. Serum Helicobacter pylori antibody reactivity in seven Asian countries using an automated latex aggregation turbidity assay. Journal of Gastroenterology and Hepatology. 2021; 36 (8):2198-2209.
Chicago/Turabian StyleJunko Akada; Evariste Tshibangu‐Kabamba; Vo Phuoc Tuan; Shusaku Kurogi; Yuichi Matsuo; Shamshul Ansari; Dalla Doohan; Bui Hoang Phuc; Phawinee Subsomwong; Langgeng Agung Waskito; Tran Thanh Binh; Lam Tung Nguyen; Vu Van Khien; Ho Dang Quy Dung; Muhammad Miftahussurur; Ari Fahrial Syam; Lotay Tshering; Ratha‐Korn Vilaichone; Varocha Mahachai; Thawee Ratanachu‐Ek; Pradeep Krishna Shrestha; Than Than Yee; Kyaw Htet; Hafeza Aftab; Takeshi Matsuhisa; Tomohisa Uchida; Tadayoshi Okimoto; Kazuhiro Mizukami; Masaaki Kodama; Kazunari Murakami; Naohiko Takahashi; Yoshio Yamaoka. 2021. "Serum Helicobacter pylori antibody reactivity in seven Asian countries using an automated latex aggregation turbidity assay." Journal of Gastroenterology and Hepatology 36, no. 8: 2198-2209.
Background 16S rRNA amplicon sequencing is an accurate method of detecting microbial infection without culture. It is unclear if sequencing has additional benefits over routine diagnostic methods for Helicobacter pylori testing. Methods We enrolled Mongolian volunteers with dyspepsia. Using routine diagnostic methods, positive H. pylori was defined as positive results on histology/immunohistochemistry, culture, rapid urease test, or serology; negative H. pylori was defined by negative results from all these tests. We performed 16S rRNA sequencing on gastric biopsy specimens and calculated cutoffs for operational taxonomic units (OTUs) and relative abundance (RA) to define positive results using ROC curves. Results We examined 161 individuals with a mean age of 43.6 years, and 64.6% were women. Using routine diagnostic methods, 122 (75.8%) participants were H. pylori positive, the sensitivity and specificity for 16S rRNA sequencing were 94.3% and 82.1% or 93.4% and 82.1% when cutoff values were set to 1113 (OTU number) or 4.4% RA, respectively (both p < .001). When combining the validated values, the concordance rate was high (91.1%); however, 16S rRNA sequencing had additional positive yield in 9 cases (5.6%) compared with routine diagnostic methods, and much greater additional positive yield compared to histopathology/IHC, culture, RUT, serology separately with 12 (7.4%), 37 (23.0%) and 43 (26.7%). Conclusion 16S rRNA amplicon sequencing detects potentially important proportion of H. pylori‐positive cases that test negative with routine diagnostic methods. The quantitative number of H. pylori can help to understand how it can be changing by diseases and RA give opportunity to understand how H. pylori communicate with other microbiota.
Boldbaatar Gantuya; Hashem B. El Serag; Batsaikhan Saruuljavkhlan; Dashdorj Azzaya; Takashi Matsumoto; Tomohisa Uchida; Khasag Oyuntsetseg; Nyamdorj Oyunbileg; Duger Davaadorj; Yoshio Yamaoka. Advantage of 16S rRNA amplicon sequencing in Helicobacter pylori diagnosis. Helicobacter 2021, 26, e12790 .
AMA StyleBoldbaatar Gantuya, Hashem B. El Serag, Batsaikhan Saruuljavkhlan, Dashdorj Azzaya, Takashi Matsumoto, Tomohisa Uchida, Khasag Oyuntsetseg, Nyamdorj Oyunbileg, Duger Davaadorj, Yoshio Yamaoka. Advantage of 16S rRNA amplicon sequencing in Helicobacter pylori diagnosis. Helicobacter. 2021; 26 (3):e12790.
Chicago/Turabian StyleBoldbaatar Gantuya; Hashem B. El Serag; Batsaikhan Saruuljavkhlan; Dashdorj Azzaya; Takashi Matsumoto; Tomohisa Uchida; Khasag Oyuntsetseg; Nyamdorj Oyunbileg; Duger Davaadorj; Yoshio Yamaoka. 2021. "Advantage of 16S rRNA amplicon sequencing in Helicobacter pylori diagnosis." Helicobacter 26, no. 3: e12790.
Helicobacter pylori (H. pylori) colonizes the human stomach and is a major cause of peptic ulcer disease and gastric cancer. However, although the prevalence of H. pylori is high in Africa, the incidence of gastric cancer is low, and this phenomenon is called to be African enigma. The CagA protein produced by H. pylori is the most studied virulence factor. The carcinogenic potential of CagA is associated with the Glu-Pro-Ile-Tyr-Ala (EPIYA) patterns and CagA-multimerization (CM) motifs. To better understand the EPIYA patterns and CM motifs of the cagA gene. Gastric mucosal biopsy specimens were obtained from 258 patients with dyspepsia living in the Dominican Republic, from which 120 H. pylori strains were cultured. After the bacterial DNA extraction, the EPIYA pattern and CM motif genotypes were determined using a polymerase chain reaction-based sequencing. The population structure of the Dominican Republic strains was analyzed using multilocus sequence typing (MLST). Peptic ulcer disease and gastric cancer were identified via endoscopy, and gastric cancer was confirmed by histopathology. Histological scores of the gastric mucosa were evaluated using the updated Sydney system. All CagA-positive strains carried the Western-type CagA according to the identified EPIYA patterns. Twenty-seven kinds of CM motifs were observed. Although the typical Western CM motif (FPLKRHDKVDDLSKVG) was observed most frequently, the typical East Asian CM motif (FPLRRSAAVNDLSKVG) was not observed. However, “FPLRRSAKVEDLSKVG”, similar to the typical East Asian CM motif, was found in 21 strains. Since this type was significantly more frequent in strains classified as hpAfrica1 using MLST analysis (P = 0.034), we termed it Africa1-CM (Af1-CM). A few hpEurope strains carried the Af1-CM motif, but they had a significantly higher ancestral Africa1 component than that of those without the Af1-CM motif (P = 0.030). In 30 cagA-positive strains, the "GKDKGPE" motif was observed immediately upstream of the EPIYA motif in the EPIYA-A segment, and there was a significant association between strains with the hpAfrica1 population and those containing the “GKDKGPE” motif (P = 0.018). In contrast, there was no significant association between the CM motif patterns and histological scores and clinical outcomes. We found the unique African CM motif in Western-type CagA and termed it Africa1-CM. The less toxicity of this motif could be one reason to explain the African enigma.
Takaaki Ono; Modesto Cruz; Hiroyuki Nagashima; Phawinee Subsomwong; Junko Akada; Takashi Matsumoto; Tomohisa Uchida; Rumiko Suzuki; Celso Hosking; José A Jiménez Abreu; Yoshio Yamaoka. Discovery of unique African Helicobacter pylori CagA-multimerization motif in the Dominican Republic. World Journal of Gastroenterology 2020, 26, 7118 -7130.
AMA StyleTakaaki Ono, Modesto Cruz, Hiroyuki Nagashima, Phawinee Subsomwong, Junko Akada, Takashi Matsumoto, Tomohisa Uchida, Rumiko Suzuki, Celso Hosking, José A Jiménez Abreu, Yoshio Yamaoka. Discovery of unique African Helicobacter pylori CagA-multimerization motif in the Dominican Republic. World Journal of Gastroenterology. 2020; 26 (45):7118-7130.
Chicago/Turabian StyleTakaaki Ono; Modesto Cruz; Hiroyuki Nagashima; Phawinee Subsomwong; Junko Akada; Takashi Matsumoto; Tomohisa Uchida; Rumiko Suzuki; Celso Hosking; José A Jiménez Abreu; Yoshio Yamaoka. 2020. "Discovery of unique African Helicobacter pylori CagA-multimerization motif in the Dominican Republic." World Journal of Gastroenterology 26, no. 45: 7118-7130.
Helicobacter pylori causes persistent infection in the gastric epithelium of more than half of the world’s population, leading to the development of severe complications such as peptic ulcer diseases, gastric cancer, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma. Several virulence factors, including cytotoxin-associated gene A (CagA), which is translocated into the gastric epithelium via the type 4 secretory system (T4SS), have been indicated to play a vital role in disease development. Although infection with strains harboring the East Asian type of CagA possessing the EPIYA-A, -B, and -D sequences has been found to potentiate cell proliferation and disease pathogenicity, the exact mechanism of CagA involvement in disease severity still remains to be elucidated. Therefore, we discuss the possible role of CagA in gastric pathogenicity.
Shamshul Ansari; Yoshio Yamaoka. Helicobacter pylori Virulence Factor Cytotoxin-Associated Gene A (CagA)-Mediated Gastric Pathogenicity. International Journal of Molecular Sciences 2020, 21, 7430 .
AMA StyleShamshul Ansari, Yoshio Yamaoka. Helicobacter pylori Virulence Factor Cytotoxin-Associated Gene A (CagA)-Mediated Gastric Pathogenicity. International Journal of Molecular Sciences. 2020; 21 (19):7430.
Chicago/Turabian StyleShamshul Ansari; Yoshio Yamaoka. 2020. "Helicobacter pylori Virulence Factor Cytotoxin-Associated Gene A (CagA)-Mediated Gastric Pathogenicity." International Journal of Molecular Sciences 21, no. 19: 7430.
ObjectiveA global consensus meeting was held to review current evidence and knowledge gaps and propose collaborative studies on population-wide screening and eradication of Helicobacter pylori for prevention of gastric cancer (GC).Methods28 experts from 11 countries reviewed the evidence and modified the statements using the Delphi method, with consensus level predefined as ≥80% of agreement on each statement. The Grading of Recommendation Assessment, Development and Evaluation (GRADE) approach was followed.ResultsConsensus was reached in 26 statements. At an individual level, eradication of H. pylori reduces the risk of GC in asymptomatic subjects and is recommended unless there are competing considerations. In cohorts of vulnerable subjects (eg, first-degree relatives of patients with GC), a screen-and-treat strategy is also beneficial. H. pylori eradication in patients with early GC after curative endoscopic resection reduces the risk of metachronous cancer and calls for a re-examination on the hypothesis of ‘the point of no return’. At the general population level, the strategy of screen-and-treat for H. pylori infection is most cost-effective in young adults in regions with a high incidence of GC and is recommended preferably before the development of atrophic gastritis and intestinal metaplasia. However, such a strategy may still be effective in people aged over 50, and may be integrated or included into national healthcare priorities, such as colorectal cancer screening programmes, to optimise the resources. Reliable locally effective regimens based on the principles of antibiotic stewardship are recommended. Subjects at higher risk of GC, such as those with advanced gastric atrophy or intestinal metaplasia, should receive surveillance endoscopy after eradication of H. pylori.ConclusionEvidence supports the proposal that eradication therapy should be offered to all individuals infected with H. pylori. Vulnerable subjects should be tested, and treated if the test is positive. Mass screening and eradication of H. pylori should be considered in populations at higher risk of GC.
Jyh-Ming Liou; Peter Malfertheiner; Yi-Chia Lee; Bor-Shyang Sheu; Kentaro Sugano; Hsiu-Chi Cheng; Khay-Guan Yeoh; Ping-I Hsu; Khean-Lee Goh; Varocha Mahachai; Takuji Gotoda; Wei-Lun Chang; Mei-Jyh Chen; Tsung-Hsien Chiang; Chieh-Chang Chen; Chun-Ying Wu; Alex Hwong-Ruey Leow; Jeng-Yih Wu; Deng-Chyang Wu; Tzu-Chan Hong; Hong Lu; Yoshio Yamaoka; Francis Megraud; Francis K L Chan; Joseph Jy Sung; Jaw-Town Lin; David Y Graham; Ming-Shiang Wu; Emad M El-Omar. Screening and eradication of Helicobacter pylori for gastric cancer prevention: the Taipei global consensus. Gut 2020, 69, 2093 -2112.
AMA StyleJyh-Ming Liou, Peter Malfertheiner, Yi-Chia Lee, Bor-Shyang Sheu, Kentaro Sugano, Hsiu-Chi Cheng, Khay-Guan Yeoh, Ping-I Hsu, Khean-Lee Goh, Varocha Mahachai, Takuji Gotoda, Wei-Lun Chang, Mei-Jyh Chen, Tsung-Hsien Chiang, Chieh-Chang Chen, Chun-Ying Wu, Alex Hwong-Ruey Leow, Jeng-Yih Wu, Deng-Chyang Wu, Tzu-Chan Hong, Hong Lu, Yoshio Yamaoka, Francis Megraud, Francis K L Chan, Joseph Jy Sung, Jaw-Town Lin, David Y Graham, Ming-Shiang Wu, Emad M El-Omar. Screening and eradication of Helicobacter pylori for gastric cancer prevention: the Taipei global consensus. Gut. 2020; 69 (12):2093-2112.
Chicago/Turabian StyleJyh-Ming Liou; Peter Malfertheiner; Yi-Chia Lee; Bor-Shyang Sheu; Kentaro Sugano; Hsiu-Chi Cheng; Khay-Guan Yeoh; Ping-I Hsu; Khean-Lee Goh; Varocha Mahachai; Takuji Gotoda; Wei-Lun Chang; Mei-Jyh Chen; Tsung-Hsien Chiang; Chieh-Chang Chen; Chun-Ying Wu; Alex Hwong-Ruey Leow; Jeng-Yih Wu; Deng-Chyang Wu; Tzu-Chan Hong; Hong Lu; Yoshio Yamaoka; Francis Megraud; Francis K L Chan; Joseph Jy Sung; Jaw-Town Lin; David Y Graham; Ming-Shiang Wu; Emad M El-Omar. 2020. "Screening and eradication of Helicobacter pylori for gastric cancer prevention: the Taipei global consensus." Gut 69, no. 12: 2093-2112.
Helicobacter pylori infection is a severe global health problem that is closely associated with acid-related diseases and gastric malignancies. Eradicating H. pylori is strongly recommended for lowering peptic ulcer recurrence and preventing gastric cancer. The current approved H. pylori eradication regimen combines a proton pump inhibitor (PPI) with two antibiotics. Unfortunately, this regimen failed to meet expectations mostly due to antibiotic resistance and insufficient gastric acid suppression. Vonoprazan, a novel potassium-competitive acid blocker, showed promising results as a PPI replacement. Vonoprazan inhibits gastric acid secretion by acting as a reversible competitive inhibitor against potassium ions and forming disulfide bonds with the cysteine molecule of H+/K+-ATPase. Vonoprazan has superior pharmacological characteristics over PPI, such as no requirement for acid activation, stability in acidic conditions, shorter optimum acid suppression period, and resistance to cytochrome P (CYP)2C19 polymorphism. Several comparative randomized controlled trials and meta-analyses revealed the superiority of vonoprazan in eradicating H. pylori, notably the resistant strains. The adverse effect caused by vonoprazan is long-term acid suppression that may induce elevated gastrin serum, hypochlorhydria, and malabsorption. All vonoprazan studies have only been conducted in Japan. Further studies outside Japan are necessary for universally conclusive results.
Muhammad Miftahussurur; Boby Pratama Putra; Yoshio Yamaoka. The Potential Benefits of Vonoprazan as Helicobacter pylori Infection Therapy. Pharmaceuticals 2020, 13, 276 .
AMA StyleMuhammad Miftahussurur, Boby Pratama Putra, Yoshio Yamaoka. The Potential Benefits of Vonoprazan as Helicobacter pylori Infection Therapy. Pharmaceuticals. 2020; 13 (10):276.
Chicago/Turabian StyleMuhammad Miftahussurur; Boby Pratama Putra; Yoshio Yamaoka. 2020. "The Potential Benefits of Vonoprazan as Helicobacter pylori Infection Therapy." Pharmaceuticals 13, no. 10: 276.
Helicobacter pylori (H.pylori) infection is etiologically associated with severe diseases including gastric cancer; but its pathogenicity is deeply shaped by the exceptional genomic diversification and geographic variation of the species. The clinical relevance of strains colonizing Africa is still debated. This study aimed to explore genomic features and virulence potentials of H. pylori KE21, a typical African strain isolated from a native Kenyan patient diagnosed with a gastric cancer. A high-quality circular genome assembly of 1,648,327 bp (1590 genes) obtained as a hybrid of Illumina Miseq short reads and Oxford Nanopore MinION long reads, clustered within hpAfrica1 population. This genome revealed a virulome and a mobilome encoding more than hundred features potentiating a successful colonization, persistent infection, and enhanced disease pathogenesis. Furthermore, through an experimental infection of gastric epithelial cell lines, strain KE21 showed the ability to promote interleukin-8 production and to induce cellular alterations resulting from the injection of a functional CagA oncogene protein into the cells. This study shows that strain KE21 is potentially virulent and can trigger oncogenic pathways in gastric epithelial cells. Expended genomic and clinical explorations are required to evaluate the epidemiological importance of H. pylori infection and its putative complications in the study population.
Catherine Mwangi; Stephen Njoroge; Evariste Tshibangu-Kabamba; Zahir Moloo; Allan Rajula; Smita Devani; Takashi Matsumoto; Kimang’A Nyerere; Samuel Kariuki; Gunturu Revathi; Yoshio Yamaoka. Whole Genome Sequencing Reveals Virulence Potentials of Helicobacter pylori Strain KE21 Isolated from a Kenyan Patient with Gastric Signet Ring Cell Carcinoma. Toxins 2020, 12, 556 .
AMA StyleCatherine Mwangi, Stephen Njoroge, Evariste Tshibangu-Kabamba, Zahir Moloo, Allan Rajula, Smita Devani, Takashi Matsumoto, Kimang’A Nyerere, Samuel Kariuki, Gunturu Revathi, Yoshio Yamaoka. Whole Genome Sequencing Reveals Virulence Potentials of Helicobacter pylori Strain KE21 Isolated from a Kenyan Patient with Gastric Signet Ring Cell Carcinoma. Toxins. 2020; 12 (9):556.
Chicago/Turabian StyleCatherine Mwangi; Stephen Njoroge; Evariste Tshibangu-Kabamba; Zahir Moloo; Allan Rajula; Smita Devani; Takashi Matsumoto; Kimang’A Nyerere; Samuel Kariuki; Gunturu Revathi; Yoshio Yamaoka. 2020. "Whole Genome Sequencing Reveals Virulence Potentials of Helicobacter pylori Strain KE21 Isolated from a Kenyan Patient with Gastric Signet Ring Cell Carcinoma." Toxins 12, no. 9: 556.
We evaluated biofilm formation of clinical Helicobacter pylori isolates from Indonesia and its relation to antibiotic resistance. We determined the minimum inhibition concentration (MIC) of amoxicillin, clarithromycin, levofloxacin, metronidazole and tetracycline by the Etest to measure the planktonic susceptibility of 101 H. pylori strains. Biofilms were quantified by the crystal violet method. The minimum biofilm eradication concentration (MBEC) was obtained by measuring the survival of bacteria in a biofilm after exposure to antibiotics. The majority of the strains formed a biofilm (93.1% (94/101)), including weak (75.5%) and strong (24.5%) biofilm-formers. Planktonic resistant and sensitive strains produced relatively equal amounts of biofilms. The resistance proportion, shown by the MBEC measurement, was higher in the strong biofilm group for all antibiotics compared to the weak biofilm group, especially for clarithromycin (p = 0.002). Several cases showed sensitivity by the MIC measurement, but resistance according to the MBEC measurements (amoxicillin, 47.6%; tetracycline, 57.1%; clarithromycin, 19.0%; levofloxacin, 38.1%; and metronidazole 38.1%). Thus, biofilm formation may increase the survival of H. pylori and its resistance to antibiotics. Biofilm-related antibiotic resistance should be evaluated with antibiotic susceptibility.
Kartika Afrida Fauzia; Muhammad Miftahussurur; Ari Fahrial Syam; Langgeng Agung Waskito; Dalla Doohan; Yudith Annisa Ayu Rezkitha; Takashi Matsumoto; Vo Phuoc Tuan; Junko Akada; Hideo Yonezawa; Shigeru Kamiya; Yoshio Yamaoka. Biofilm Formation and Antibiotic Resistance Phenotype of Helicobacter pylori Clinical Isolates. Toxins 2020, 12, 473 .
AMA StyleKartika Afrida Fauzia, Muhammad Miftahussurur, Ari Fahrial Syam, Langgeng Agung Waskito, Dalla Doohan, Yudith Annisa Ayu Rezkitha, Takashi Matsumoto, Vo Phuoc Tuan, Junko Akada, Hideo Yonezawa, Shigeru Kamiya, Yoshio Yamaoka. Biofilm Formation and Antibiotic Resistance Phenotype of Helicobacter pylori Clinical Isolates. Toxins. 2020; 12 (8):473.
Chicago/Turabian StyleKartika Afrida Fauzia; Muhammad Miftahussurur; Ari Fahrial Syam; Langgeng Agung Waskito; Dalla Doohan; Yudith Annisa Ayu Rezkitha; Takashi Matsumoto; Vo Phuoc Tuan; Junko Akada; Hideo Yonezawa; Shigeru Kamiya; Yoshio Yamaoka. 2020. "Biofilm Formation and Antibiotic Resistance Phenotype of Helicobacter pylori Clinical Isolates." Toxins 12, no. 8: 473.
We aimed to validate 2 types of antibodies, anti-CagA antibody and anti-East Asian CagA specific antibody (α-EAS antibody) for the determination of CagA status in Indonesia. We also confirmed the performance of α-EAS antibody for the detection of East Asian-type CagA H. pylori. Immunohistochemistry was performed using anti-CagA antibody and α-EAS antibody on gastric biopsy specimens from a total of 967 Indonesian patients. Diagnostic values of immunohistochemistry were evaluated with PCR-based sequencing as gold standard. Anti-CagA antibody had high sensitivity, specificity, and accuracy (87.0 %, 100 %, and 98.8 %, respectively) for determining CagA status. The α-EAS antibody was not suitable for the purpose of CagA status determination, as it had a low sensitivity (23.9 %). High specificity (97.6 %) but low sensitivity (41.2 %) and accuracy (66.3 %) was observed in α-EAS antibody to detect East Asian-type CagA. Patients with positive result of immunohistochemistry using anti-CagA antibody had significantly higher monocyte infiltration score in antrum (P < 0.001) and corpus (P = 0.009). In conclusion, the anti-CagA antibody is still suitable to be used in Indonesia for determining the CagA status, whilst the α-EAS antibody was not appropriate to discriminate between East Asian-type and non-East Asian-type CagA in Indonesia.
Muhammad Miftahussurur; Dalla Doohan; Ari Fahrial Syam; Iswan Abbas Nusi; Langgeng Agung Waskito; Kartika Afrida Fauzia; Yudith Annisa Ayu Rezkitha; Astri Dewayani; Reny I'Tishom; Hasan Maulahela; Tomohisa Uchida; Yoshio Yamaoka. The validation of the Helicobacter pylori CagA typing by immunohistochemistry: nationwide application in Indonesia. Acta Histochemica 2020, 122, 151594 .
AMA StyleMuhammad Miftahussurur, Dalla Doohan, Ari Fahrial Syam, Iswan Abbas Nusi, Langgeng Agung Waskito, Kartika Afrida Fauzia, Yudith Annisa Ayu Rezkitha, Astri Dewayani, Reny I'Tishom, Hasan Maulahela, Tomohisa Uchida, Yoshio Yamaoka. The validation of the Helicobacter pylori CagA typing by immunohistochemistry: nationwide application in Indonesia. Acta Histochemica. 2020; 122 (6):151594.
Chicago/Turabian StyleMuhammad Miftahussurur; Dalla Doohan; Ari Fahrial Syam; Iswan Abbas Nusi; Langgeng Agung Waskito; Kartika Afrida Fauzia; Yudith Annisa Ayu Rezkitha; Astri Dewayani; Reny I'Tishom; Hasan Maulahela; Tomohisa Uchida; Yoshio Yamaoka. 2020. "The validation of the Helicobacter pylori CagA typing by immunohistochemistry: nationwide application in Indonesia." Acta Histochemica 122, no. 6: 151594.
Mongolia has a high prevalence of Helicobacter pylori infection and the second highest incidence of gastric cancer worldwide. Thus, investigating the prevalence of antibiotic resistance and its underlying genetic mechanism is necessary. We isolated 361 H. pylori strains throughout Mongolia. Agar dilution assays were used to determine the minimum inhibitory concentrations of five antibiotics; amoxicillin, clarithromycin, metronidazole, levofloxacin, and minocycline. The genetic determinants of antibiotic resistance were identified with next-generation sequencing (NGS) and the CLC Genomics Workbench. The resistance to metronidazole, levofloxacin, clarithromycin, amoxicillin, and minocycline was 78.7%, 41.3%, 29.9%, 11.9% and 0.28%, respectively. Multidrug resistance was identified in 51.3% of the isolates investigated which were further delineated into 9 antimicrobial resistance profiles. A number of known antibiotic resistance mutations were identified including rdxA, frxA (missense, frameshift), gyrA (N87K, A88P, D91G/N/Y), 23S rRNA (A2143G), pbp1A (N562Y), and 16S rRNA (A928C). Furthermore, we detected previously unreported mutations in pbp1A (L610*) and the 23S rRNA gene (A1410G, C1707T, A2167G, C2248T, and C2922T). The degree of antibiotic resistance was high, indicating the insufficiency of standard triple therapy in Mongolia.
Dashdorj Azzaya; Boldbaatar Gantuya; Khasag Oyuntsetseg; Duger Davaadorj; Takashi Matsumoto; Junko Akada; Yoshio Yamaoka. High Antibiotic Resistance of Helicobacter pylori and Its Associated Novel Gene Mutations among the Mongolian Population. Microorganisms 2020, 8, 1062 .
AMA StyleDashdorj Azzaya, Boldbaatar Gantuya, Khasag Oyuntsetseg, Duger Davaadorj, Takashi Matsumoto, Junko Akada, Yoshio Yamaoka. High Antibiotic Resistance of Helicobacter pylori and Its Associated Novel Gene Mutations among the Mongolian Population. Microorganisms. 2020; 8 (7):1062.
Chicago/Turabian StyleDashdorj Azzaya; Boldbaatar Gantuya; Khasag Oyuntsetseg; Duger Davaadorj; Takashi Matsumoto; Junko Akada; Yoshio Yamaoka. 2020. "High Antibiotic Resistance of Helicobacter pylori and Its Associated Novel Gene Mutations among the Mongolian Population." Microorganisms 8, no. 7: 1062.