Verticillium wilt is characterized by chlorosis in leaves and is a devastating disease in eggplant. Early diagnosis, prior to the manifestation of symptoms, enables targeted management of the disease. In this study, we aim to detect early leaf wilt in eggplant leaves caused by Verticillium dahliae by integrating multispectral imaging with machine learning and deep learning techniques. Multispectral and chlorophyll fluorescence images were collected from leaves of the inbred eggplant line 11-435, including data on image texture, spectral reflectance, and chlorophyll fluorescence. Subsequently, we established a multispectral data model, fusion information model, and multispectral image–information fusion model. The multispectral image–information fusion model, integrated with a two-dimensional convolutional neural network (2D-CNN), demonstrated optimal performance in classifying early-stage Verticillium wilt infection, achieving a test accuracy of 99.37%. Additionally, transfer learning enabled us to diagnose early leaf wilt in another eggplant variety, the inbred line 14-345, with an accuracy of 84.54 ± 1.82%. Compared to traditional methods that rely on visible symptom observation and typically require about 10 days to confirm infection, this study achieved early detection of Verticillium wilt as soon as the third day post-inoculation. These findings underscore the potential of the fusion model as a valuable tool for the early detection of pre-symptomatic states in infected plants, thereby offering theoretical support for in-field detection of eggplant health.

Broccoli is a highly nutritious vegetable that is favored worldwide. Assessing and predicting the shelf life of broccoli holds considerable importance for effective resource optimization and management. The physicochemical parameters and spectral characteristics of broccoli are important indicators partially reflecting its shelf life. However, few studies have used spectral image information to predict and evaluate the shelf life of broccoli. In this study, multispectral imaging combined with multi-feature data fusion was used to predict and evaluate the shelf life of broccoli. Spectral data and textural features were extracted from multispectral images of broccoli and fused with the physicochemical parameters for analysis. Savitzky–Golay (SG) convolution smoothing and standard normal variate (SNV) and normalization (Norm) preprocessing methods were employed to preprocess the original spectral data and textural features, while a successive projection algorithm (SPA) was used to extract relevant feature bands. The physicochemical parameters for broccoli shelf life were predicted using three methods: support vector regression (SVR), random forest classification (RF), and 2D convolutional neural network (2D-CNN) models. Broccoli shelf life prediction models were evaluated using three classification methods: RF, 1D-CNN, and 2D-CNN. The results demonstrate that, among the models used for predicting and evaluating the shelf life of broccoli, the SPA+SG+RF classification model employing fused data Type C achieves the highest accuracy. Specifically, this method achieves accuracies of 88.98% and 88.64% for the training and validation sets, respectively. Multi-feature data fusion of spectral image information and physical and chemical parameters were combined with different machine learning methods to predict and evaluate the shelf life of broccoli.

For different broccoli materials, it used to be necessary to manually plant in a large area for the investigation of flower ball information, and this method is susceptible to subjective influence, which is not only time-consuming and laborious but may also cause some damage to the broccoli in the process of investigation. Therefore, the rapid and nondestructive monitoring of flower heads is key to acquiring high-throughput phenotypic information on broccoli crops. In this study, we used an unmanned aerial vehicle (UAV) to acquire hundreds of images of field-grown broccoli to evaluate their flower head development rate and sizes during growth. First, YOLOv5 and YOLOv8 were used to complete the position detection and counting statistics at the seedling and heading stages. Then, UNet, PSPNet, DeepLabv3+, and SC-DeepLabv3+ were used to segment the flower heads in the images. The improved SC-DeepLabv3+ model excelled in segmenting flower heads, showing Precision, reconciled mean F1-score, mean intersection over union, and mean pixel accuracy values of 93.66%, 95.24%, 91.47%, and 97.24%, respectively, which were 0.57, 1.12, 1.16, and 1.70 percentage points higher than the respective values achieved with the DeepLabv3+ model. Flower head sizes were predicted on the basis of the pixel value of individual flower heads and ground sampling distance, yielding predictions with an R² value of 0.67 and root-mean-squared error of 1.81 cm. Therefore, the development rate and sizes of broccoli flower heads during growth were successively estimated and calculated. Compared with the existing technology, it greatly improves work efficiency and can help to obtain timely information on crop growth in the field. Our methodology provides a convenient, fast, and reliable way for investigating field traits in broccoli breeding.

The seed-germination percentage is an important indicator of the seed viability and growth potential and has important implications for plant breeding and agricultural production. Thus, to increase the speed and accuracy in measuring the completion of germination in experimental seed batches for precise germination percentage calculation, we evaluated a You-Only-Look-Once (YOLO)–Seed Germination Detection (SGD) algorithm that integrates deep-learning technology and texture feature-extraction mechanisms specific to germinating seeds. The algorithm was built upon YOLOv7-l, and its applicability was optimised based on the results of our germination experiments. In the backbone network, an internal convolution structure was substituted to enhance the spatial specificity of the initial features. Following the output of the main feature-extraction network, an Explicit Visual Centre (EVC) module was introduced to mitigate the interference caused by intertwined primary roots from germinated seeds, which can affect recognition accuracy. Furthermore, a Spatial Context Pyramid (SCP) module was embedded after enhancing the feature-extraction network to improve the model’s accuracy in identifying seeds of different scales, particularly in recognising small target seeds. Our results with cabbage seeds showed that the YOLO–SGD model, with a model size of 45.22 M, achieved an average detection accuracy of 99.6% for large-scale seeds and 96.4% for small-scale seeds. The model also achieved a mean average precision and F1 score of 98.0% and 93.3%, respectively. Compared with manual germination-rate detection, the model maintained an average absolute error of prediction within 1.0%, demonstrating sufficient precision to replace manual methods in laboratory environments and efficiently detect germinated seeds for precise germination percentage assessment.

Cauliflower emergence rate and seedling growth are key indicators of field conditions and varietal potential. Traditional manual surveys are unsuitable for continuous monitoring across multiple varieties. This study integrates UAV RGB imagery with the DualSlim-YOLO model to estimate cauliflower emergence rates and monitor seedling growth. Built on YOLOv11, the model incorporates a lightweight feature extraction structure and an optimized detection-scale configuration. It reduces computational complexity while maintaining detection accuracy, thereby improving the efficiency of cauliflower seedling detection. DualSlim-YOLO achieved P, R, F1-score, mAP@0.5, and mAP@0.5:0.95 of 95.35%, 96.75%, 96.05%, 98.55%, and 86.65%, respectively. The number of parameters was reduced by 38.61%, while the inference speed increased by 22.16%, demonstrating good lightweight performance. Based on this model, UAV images of 171 cauliflower varieties acquired at 7, 21, and 28 d after transplanting were used for seedling detection and emergence rate estimation. In addition, 18 time-series seedling phenotypic traits were extracted, enabling a comprehensive quantitative evaluation of emergence dynamics and early-growth performance across multiple cauliflower varieties. This method effectively screens cauliflower varieties for high emergence rates, rapid emergence, and excellent seedling growth performance. It provides technical support for high-throughput, nondestructive seedling phenotyping and early germplasm screening under field conditions.