Sustainable agriculture is considered a crucial solution for developing technologies and practices that do not harm the environment, are accessible to farmers, and enhance food production. The current lifestyle of societies is widely regarded as the primary cause of environmental issues, leading to a slowdown in overall development, which must be addressed to ensure ecological sustainability. However, societal needs cannot be fully met based solely on the environmental dimension; economic, social, and cultural factors are equally important. This indicates that new approaches are necessary, which integrate biological and ecological processes into food production, reduce the use of non-renewable resources that harm both the environment and human health and effectively leverage the knowledge and capacities of local farmers. The growing interest in bioregions, grounded in agroecology principles, encompasses economic, environmental, and social challenges. It is part of broader discussions on the integration of food systems with local territories and cultural values, aiming to improve the quality of life in rural communities by making agriculture more profitable, creating new markets, promoting local tourism, reducing agriculture’s environmental impact, conserving biodiversity, and encouraging local food consumption. However, a key question remains: can agroecology coexist with a highly formalized agricultural model without compromising its sustainability potential? Therefore, this study aimed to synthesise and analyse scientific knowledge on the concept of bioregions as a means of implementation agroecology, with a particular focus on its impact on local economies, social cohesion, and environmental sustainability.