Background. Trichomonas vaginalis (T. vaginalis) infection has been long considered among the sexually transmitted diseases that possesses a clear effect on women’s health especially in the childbearing period. Methods. A 234 females of age range 18-45 years old attending the Gynecology and Obstetrics Outpatient Clinic of Kasr El Aini Hospitals were enrolled in a cross-sectional study. The taken vaginal swabs were subjected to wet mount microscopy, Giemsa stain, modified Diamond’s culture, and nested polymerase chain reaction (nPCR) amplification. Multiattribute and analytical hierarchy processes were conducted to detect laboratory utility. Univariate and multivariate analyses were done to detect the multiple risk factors that may be associated with Trichomonas infection. Results. Based on nPCR, the prevalence of trichomoniasis was 26.9%. Wet mount, Giemsa stain, and culture showed 100% specificity but of low sensitivity (28.57%, 28.57%, and 57.14%, respectively). On the multivariate analysis, nPCR showed the highest rank for diagnostic performance and culture had the lowest rank. For univariate analysis, there was a significant correlation between T. vaginalis infection and vaginal discharge, burning sensation, dyspareunia, and the use of intrauterine device (IUD) ( value < 0.05). Conclusion. The routine screening of trichomoniasis using nPCR was reliable, sensitive, and specific. Also, it could financially be considered a more suitable option in batch screening. Significant higher rates of infection were reported among IUD users compared to condom or hormonal-based methods.

Toxoplasma gondii , an opportunistic protozoan, is widely prevalent and poses potential health risks. Its treatment remains challenging with several side effects, urging the exploration of new, safe, and efficient drugs. We aimed to explore the therapeutic efficacy of the medicinal plant Garcinia cambogia (GC) against both acute and chronic toxoplasmosis. Sixty Swiss albino mice were allocated into five groups: I (healthy), II (model), III (spiramycin [SPM] treated, IV (GC treated), and V (SPM + GC treated). Each group is further divided into two subgroups receiving treatment at 4 and 42 days postinfection (dpi) for acute and chronic infection, respectively. To determine the properties of the GC active compounds, hydroxycitric acid (HCA), guttiferone‐E, guttiferone‐J, compared to SPM against the selective T . gondii ROP18 transferase target regarding its binding affinities, and estimated inhibition constant, Lipinski’s RO5 filter analyses and computational QSARs/ADMET predictive characteristics were used. The groups were evaluated parasitologically, histopathologically, and immunohistochemically for Iba‐1 expression. The best treatment outcome was observed in the SPM + GC–treated groups in both acute and chronic infections, with inhibition percentages of 89% and 78%, respectively, and preservation of neuronal integrity, as evidenced by the lowest Iba1 expression levels. Moreover, the docked ROP18‐HCA revealed an additional, critical, and novel ligand‐binding site for ROP18, which is essential for the virulence and pathogenesis of Toxoplasma . G. cambogia ’s stability of intermolecular binding activities, potency, and conformations makes it an effective, safe, and promising remedy for treating toxoplasmosis, especially in combination with SPM.